Load time: 03/06/2013 11:20:56 AM Read time: 03/06/2013 11:20:56 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2958 Comment: plate1_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 105311376 cps Min counts: 1632 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 50650.00 65647508.00 56822692.00 17032250.00 25227938.00 7824187.00 23679486.00 29636384.00 3398.00 3322.00 B 54274.00 31943586.00 27257498.00 16002236.00 7974778.00 12090382.00 34507712.00 16663768.00 3511.00 3530.00 C 54614.00 20259086.00 10619449.00 5103754.00 21552714.00 33586220.00 5772637.00 3245210.00 3303.00 3473.00 D 55180.00 9690199.00 57325248.00 10072147.00 105311376.00 4108521.00 3508280.00 8797335.00 3492.00 3303.00 E 6160113.00 23328712.00 9014872.00 5110662.00 44617800.00 2431469.00 30967238.00 3416.00 3530.00 F 2063186.00 7736696.00 12844601.00 19099686.00 9512086.00 19117014.00 10299672.00 3662.00 3265.00 G 1325661.00 22589270.00 8849428.00 17810390.00 8401226.00 6409880.00 10585171.00 3492.00 3492.00 H 834414.00 14531284.00 17258710.00 20321220.00 13027439.00 15777401.00 11861155.00 3454.00 3549.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 65593828.50 56769012.50 16978570.50 25174258.50 7770507.50 23625806.50 29582704.50 -50281.50 -50357.50 B 31889906.50 27203818.50 15948556.50 7921098.50 12036702.50 34454032.50 16610088.50 -50168.50 -50149.50 C 20205406.50 10565769.50 5050074.50 21499034.50 33532540.50 5718957.50 3191530.50 -50376.50 -50206.50 D 9636519.50 57271568.50 10018467.50 105257696.50 4054841.50 3454600.50 8743655.50 -50187.50 -50376.50 E 6106433.50 23275032.50 8961192.50 5056982.50 44564120.50 2377789.50 30913558.50 -50263.50 -50149.50 F 2009506.50 7683016.50 12790921.50 19046006.50 9458406.50 19063334.50 10245992.50 -50017.50 -50414.50 G 1271981.50 22535590.50 8795748.50 17756710.50 8347546.50 6356200.50 10531491.50 -50187.50 -50187.50 H 780734.50 14477604.50 17205030.50 20267540.50 12973759.50 15723721.50 11807475.50 -50225.50 -50130.50 Load time: 03/06/2013 11:23:27 AM Read time: 03/06/2013 11:23:27 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2959 Comment: plate2_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 100937096 cps Min counts: 1529 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 98713.00 66255000.00 59255056.00 41031388.00 100937096.00 47827360.00 21602026.00 87788384.00 3284.00 3228.00 B 39587.00 37678836.00 81009184.00 18729760.00 80816992.00 27229660.00 27625204.00 7240951.00 3511.00 3133.00 C 49498.00 21274018.00 18192934.00 27462144.00 41671324.00 36830320.00 11322123.00 26243674.00 3530.00 3171.00 D 50725.00 10397504.00 10504015.00 25978460.00 27474342.00 31297260.00 41333408.00 8409558.00 3398.00 3058.00 E 6181668.00 3112053.00 60716016.00 17705462.00 48649440.00 49065568.00 44379144.00 3228.00 3435.00 F 2074031.00 16948774.00 42514700.00 42018228.00 27096740.00 29661848.00 60033452.00 3492.00 3379.00 G 1355375.00 10860618.00 29012136.00 55326908.00 51660820.00 77941400.00 39417888.00 3265.00 3152.00 H 607706.00 17508538.00 19406664.00 42260732.00 14797632.00 78303680.00 38791812.00 3209.00 3284.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 66195369.25 59195425.25 40971757.25 100877465.25 47767729.25 21542395.25 87728753.25 -56346.75 -56402.75 B 37619205.25 80949553.25 18670129.25 80757361.25 27170029.25 27565573.25 7181320.25 -56119.75 -56497.75 C 21214387.25 18133303.25 27402513.25 41611693.25 36770689.25 11262492.25 26184043.25 -56100.75 -56459.75 D 10337873.25 10444384.25 25918829.25 27414711.25 31237629.25 41273777.25 8349927.25 -56232.75 -56572.75 E 6122037.25 3052422.25 60656385.25 17645831.25 48589809.25 49005937.25 44319513.25 -56402.75 -56195.75 F 2014400.25 16889143.25 42455069.25 41958597.25 27037109.25 29602217.25 59973821.25 -56138.75 -56251.75 G 1295744.25 10800987.25 28952505.25 55267277.25 51601189.25 77881769.25 39358257.25 -56365.75 -56478.75 H 548075.25 17448907.25 19347033.25 42201101.25 14738001.25 78244049.25 38732181.25 -56421.75 -56346.75 Load time: 03/06/2013 11:25:21 AM Read time: 03/06/2013 11:25:21 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2960 Comment: plate1_middle Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 59106948 cps Min counts: 1170 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27411.00 38422872.00 31281686.00 9516509.00 13047244.00 4478300.00 13199586.00 16917180.00 2435.00 2491.00 B 29091.00 17287880.00 15086929.00 9009105.00 4430445.00 6726260.00 19639312.00 9562516.00 2510.00 2529.00 C 28411.00 10797770.00 6025054.00 2890284.00 12008890.00 18708416.00 3274122.00 1850020.00 2340.00 2454.00 D 28638.00 5168036.00 30861070.00 5701462.00 59106948.00 2335206.00 2000905.00 5055814.00 2605.00 2435.00 E 3322813.00 13049688.00 5032848.00 2872862.00 25612918.00 1415011.00 16878960.00 2718.00 2680.00 F 1116718.00 4398935.00 7286703.00 10717759.00 5387018.00 10975791.00 5755489.00 2699.00 2605.00 G 736606.00 12435173.00 4949302.00 9698987.00 4800906.00 3669872.00 5943067.00 2491.00 2435.00 H 457946.00 8432468.00 9807550.00 11456284.00 7500323.00 8916112.00 6855121.00 2605.00 2718.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 38394484.25 31253298.25 9488121.25 13018856.25 4449912.25 13171198.25 16888792.25 -25952.75 -25896.75 B 17259492.25 15058541.25 8980717.25 4402057.25 6697872.25 19610924.25 9534128.25 -25877.75 -25858.75 C 10769382.25 5996666.25 2861896.25 11980502.25 18680028.25 3245734.25 1821632.25 -26047.75 -25933.75 D 5139648.25 30832682.25 5673074.25 59078560.25 2306818.25 1972517.25 5027426.25 -25782.75 -25952.75 E 3294425.25 13021300.25 5004460.25 2844474.25 25584530.25 1386623.25 16850572.25 -25669.75 -25707.75 F 1088330.25 4370547.25 7258315.25 10689371.25 5358630.25 10947403.25 5727101.25 -25688.75 -25782.75 G 708218.25 12406785.25 4920914.25 9670599.25 4772518.25 3641484.25 5914679.25 -25896.75 -25952.75 H 429558.25 8404080.25 9779162.25 11427896.25 7471935.25 8887724.25 6826733.25 -25782.75 -25669.75 Load time: 03/06/2013 11:28:31 AM Read time: 03/06/2013 11:28:31 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2962 Comment: plate1_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 59821664 cps Min counts: 1170 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26863.00 38963828.00 32507614.00 9674220.00 13154964.00 4476550.00 13435399.00 17019804.00 2340.00 2473.00 B 28902.00 17190030.00 15533262.00 9036194.00 4584003.00 6794148.00 20126730.00 9549747.00 2454.00 2416.00 C 27580.00 10530335.00 6002613.00 2876370.00 12267482.00 19081492.00 3273243.00 1845612.00 2454.00 2397.00 D 30771.00 5103185.00 31500362.00 5713781.00 59821664.00 2322490.00 2033617.00 5038315.00 2624.00 2586.00 E 3337804.00 12991609.00 5074084.00 2918718.00 25302150.00 1413218.00 17253202.00 2359.00 2642.00 F 1123079.00 4591207.00 7363909.00 10741880.00 5489243.00 11222297.00 5783301.00 2661.00 2605.00 G 731320.00 12555329.00 5146134.00 10037982.00 4823950.00 3665506.00 5952926.00 2642.00 2642.00 H 450243.00 8603057.00 10126572.00 11638880.00 7572356.00 8881129.00 7044924.00 2586.00 2435.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 38935299.00 32479085.00 9645691.00 13126435.00 4448021.00 13406870.00 16991275.00 -26189.00 -26056.00 B 17161501.00 15504733.00 9007665.00 4555474.00 6765619.00 20098201.00 9521218.00 -26075.00 -26113.00 C 10501806.00 5974084.00 2847841.00 12238953.00 19052963.00 3244714.00 1817083.00 -26075.00 -26132.00 D 5074656.00 31471833.00 5685252.00 59793135.00 2293961.00 2005088.00 5009786.00 -25905.00 -25943.00 E 3309275.00 12963080.00 5045555.00 2890189.00 25273621.00 1384689.00 17224673.00 -26170.00 -25887.00 F 1094550.00 4562678.00 7335380.00 10713351.00 5460714.00 11193768.00 5754772.00 -25868.00 -25924.00 G 702791.00 12526800.00 5117605.00 10009453.00 4795421.00 3636977.00 5924397.00 -25887.00 -25887.00 H 421714.00 8574528.00 10098043.00 11610351.00 7543827.00 8852600.00 7016395.00 -25943.00 -26094.00 Load time: 03/06/2013 11:30:27 AM Read time: 03/06/2013 11:30:27 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2963 Comment: plate2-2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 55090788 cps Min counts: 1151 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 53236.00 38181168.00 32354050.00 33036632.00 55090788.00 26695882.00 12057370.00 49642972.00 2642.00 2529.00 B 20916.00 20954342.00 42315776.00 15134441.00 44046464.00 15386750.00 15181836.00 4090870.00 2624.00 2322.00 C 25881.00 11301568.00 9681647.00 22265286.00 23536268.00 20716924.00 6389610.00 14438893.00 2454.00 2416.00 D 25938.00 5437151.00 5544672.00 20800070.00 15113349.00 17828378.00 23212390.00 4610839.00 2378.00 2491.00 E 3365754.00 1742958.00 50608276.00 9854057.00 27590412.00 27281056.00 26013068.00 2510.00 2378.00 F 1139655.00 9045877.00 34313692.00 23293434.00 15117605.00 16577671.00 33630192.00 2416.00 2359.00 G 731169.00 6059402.00 23384660.00 31480740.00 29064808.00 44540440.00 22628076.00 2454.00 2510.00 H 327214.00 9576632.00 16249400.00 23877056.00 8537601.00 44126140.00 22160636.00 2454.00 2303.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 38149675.25 32322557.25 33005139.25 55059295.25 26664389.25 12025877.25 49611479.25 -28850.75 -28963.75 B 20922849.25 42284283.25 15102948.25 44014971.25 15355257.25 15150343.25 4059377.25 -28868.75 -29170.75 C 11270075.25 9650154.25 22233793.25 23504775.25 20685431.25 6358117.25 14407400.25 -29038.75 -29076.75 D 5405658.25 5513179.25 20768577.25 15081856.25 17796885.25 23180897.25 4579346.25 -29114.75 -29001.75 E 3334261.25 1711465.25 50576783.25 9822564.25 27558919.25 27249563.25 25981575.25 -28982.75 -29114.75 F 1108162.25 9014384.25 34282199.25 23261941.25 15086112.25 16546178.25 33598699.25 -29076.75 -29133.75 G 699676.25 6027909.25 23353167.25 31449247.25 29033315.25 44508947.25 22596583.25 -29038.75 -28982.75 H 295721.25 9545139.25 16217907.25 23845563.25 8506108.25 44094647.25 22129143.25 -29038.75 -29189.75 Load time: 03/06/2013 11:30:27 AM Read time: 03/06/2013 11:30:27 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2963 Comment: plate2-2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 55090788 cps Min counts: 1151 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 53236.00 38181168.00 32354050.00 33036632.00 55090788.00 26695882.00 12057370.00 49642972.00 2642.00 2529.00 B 20916.00 20954342.00 42315776.00 15134441.00 44046464.00 15386750.00 15181836.00 4090870.00 2624.00 2322.00 C 25881.00 11301568.00 9681647.00 22265286.00 23536268.00 20716924.00 6389610.00 14438893.00 2454.00 2416.00 D 25938.00 5437151.00 5544672.00 20800070.00 15113349.00 17828378.00 23212390.00 4610839.00 2378.00 2491.00 E 3365754.00 1742958.00 50608276.00 9854057.00 27590412.00 27281056.00 26013068.00 2510.00 2378.00 F 1139655.00 9045877.00 34313692.00 23293434.00 15117605.00 16577671.00 33630192.00 2416.00 2359.00 G 731169.00 6059402.00 23384660.00 31480740.00 29064808.00 44540440.00 22628076.00 2454.00 2510.00 H 327214.00 9576632.00 16249400.00 23877056.00 8537601.00 44126140.00 22160636.00 2454.00 2303.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 38149675.25 32322557.25 33005139.25 55059295.25 26664389.25 12025877.25 49611479.25 -28850.75 -28963.75 B 20922849.25 42284283.25 15102948.25 44014971.25 15355257.25 15150343.25 4059377.25 -28868.75 -29170.75 C 11270075.25 9650154.25 22233793.25 23504775.25 20685431.25 6358117.25 14407400.25 -29038.75 -29076.75 D 5405658.25 5513179.25 20768577.25 15081856.25 17796885.25 23180897.25 4579346.25 -29114.75 -29001.75 E 3334261.25 1711465.25 50576783.25 9822564.25 27558919.25 27249563.25 25981575.25 -28982.75 -29114.75 F 1108162.25 9014384.25 34282199.25 23261941.25 15086112.25 16546178.25 33598699.25 -29076.75 -29133.75 G 699676.25 6027909.25 23353167.25 31449247.25 29033315.25 44508947.25 22596583.25 -29038.75 -28982.75 H 295721.25 9545139.25 16217907.25 23845563.25 8506108.25 44094647.25 22129143.25 -29038.75 -29189.75 Load time: 03/06/2013 11:32:36 AM Read time: 03/06/2013 11:32:36 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2964 Comment: plate2-2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 107576464 cps Min counts: 1755 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 105491.00 74438168.00 62952260.00 37324544.00 107576464.00 51710568.00 22820404.00 93950336.00 3756.00 3794.00 B 40248.00 39781292.00 82633240.00 17134492.00 84908952.00 29639908.00 28285796.00 7845256.00 4172.00 3624.00 C 48592.00 21834924.00 18770774.00 25428542.00 44886652.00 39755524.00 11974060.00 27771352.00 4058.00 3718.00 D 49064.00 10486607.00 10637521.00 23628980.00 29353998.00 33723028.00 44094936.00 8725857.00 3794.00 3605.00 E 6285355.00 3342252.00 56144000.00 18776424.00 52320076.00 51969840.00 48360092.00 3681.00 3605.00 F 2135347.00 17500494.00 38477752.00 45428840.00 29023570.00 31440422.00 63672800.00 3662.00 3567.00 G 1361416.00 11634818.00 26180760.00 59018968.00 55497652.00 85475864.00 42551432.00 3794.00 3511.00 H 630813.00 18660652.00 18046914.00 45387512.00 16035452.00 83827376.00 41590404.00 3718.00 3662.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 74377319.25 62891411.25 37263695.25 107515615.25 51649719.25 22759555.25 93889487.25 -57092.75 -57054.75 B 39720443.25 82572391.25 17073643.25 84848103.25 29579059.25 28224947.25 7784407.25 -56676.75 -57224.75 C 21774075.25 18709925.25 25367693.25 44825803.25 39694675.25 11913211.25 27710503.25 -56790.75 -57130.75 D 10425758.25 10576672.25 23568131.25 29293149.25 33662179.25 44034087.25 8665008.25 -57054.75 -57243.75 E 6224506.25 3281403.25 56083151.25 18715575.25 52259227.25 51908991.25 48299243.25 -57167.75 -57243.75 F 2074498.25 17439645.25 38416903.25 45367991.25 28962721.25 31379573.25 63611951.25 -57186.75 -57281.75 G 1300567.25 11573969.25 26119911.25 58958119.25 55436803.25 85415015.25 42490583.25 -57054.75 -57337.75 H 569964.25 18599803.25 17986065.25 45326663.25 15974603.25 83766527.25 41529555.25 -57130.75 -57186.75 Load time: 03/06/2013 11:34:08 AM Read time: 03/06/2013 11:34:08 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2965 Comment: plate1-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 112392448 cps Min counts: 1859 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 52424.00 74378744.00 62503944.00 18361242.00 30334190.00 8606687.00 25779860.00 31746862.00 3737.00 3907.00 B 55067.00 32194418.00 28402580.00 17258554.00 8924250.00 12897304.00 37862408.00 18078556.00 3888.00 3888.00 C 54935.00 20660576.00 11529224.00 5426246.00 23323434.00 35342380.00 6247698.00 3513237.00 3718.00 3870.00 D 55860.00 10101992.00 59592988.00 10892885.00 112392448.00 4366131.00 3821296.00 9566714.00 4115.00 3832.00 E 6504700.00 24507438.00 9631863.00 5532093.00 47138104.00 2741969.00 34363712.00 3926.00 4002.00 F 2110418.00 8520329.00 14005720.00 20164310.00 10467400.00 20740264.00 10983679.00 3945.00 4058.00 G 1381314.00 24105720.00 9720654.00 18826620.00 9025974.00 6875382.00 11358613.00 4134.00 3794.00 H 861693.00 15998561.00 18574942.00 22058352.00 14176554.00 16761330.00 13301238.00 3813.00 4096.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 74324172.50 62449372.50 18306670.50 30279618.50 8552115.50 25725288.50 31692290.50 -50834.50 -50664.50 B 32139846.50 28348008.50 17203982.50 8869678.50 12842732.50 37807836.50 18023984.50 -50683.50 -50683.50 C 20606004.50 11474652.50 5371674.50 23268862.50 35287808.50 6193126.50 3458665.50 -50853.50 -50701.50 D 10047420.50 59538416.50 10838313.50 112337876.50 4311559.50 3766724.50 9512142.50 -50456.50 -50739.50 E 6450128.50 24452866.50 9577291.50 5477521.50 47083532.50 2687397.50 34309140.50 -50645.50 -50569.50 F 2055846.50 8465757.50 13951148.50 20109738.50 10412828.50 20685692.50 10929107.50 -50626.50 -50513.50 G 1326742.50 24051148.50 9666082.50 18772048.50 8971402.50 6820810.50 11304041.50 -50437.50 -50777.50 H 807121.50 15943989.50 18520370.50 22003780.50 14121982.50 16706758.50 13246666.50 -50758.50 -50475.50 Load time: 03/06/2013 11:35:32 AM Read time: 03/06/2013 11:35:33 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2966 Comment: plate1-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 112123552 cps Min counts: 1783 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 49574.00 75188032.00 63610988.00 18620254.00 29890598.00 8607377.00 25788200.00 31639566.00 3851.00 3700.00 B 52839.00 31438630.00 28562360.00 17305950.00 8904468.00 12794973.00 38163828.00 18039460.00 4021.00 3832.00 C 52556.00 19691978.00 11471309.00 5443911.00 23255256.00 35810660.00 6230431.00 3504972.00 3907.00 3756.00 D 52613.00 9537405.00 60017604.00 10913748.00 112123552.00 4395722.00 3823353.00 9483236.00 4002.00 3567.00 E 6348153.00 24490900.00 9775080.00 5575504.00 47212656.00 2741209.00 34479244.00 3983.00 3870.00 F 2053776.00 8663464.00 13878672.00 20164414.00 10516172.00 20740318.00 11013812.00 4039.00 3907.00 G 1343595.00 24134686.00 9750734.00 18846030.00 9085917.00 6854381.00 11434551.00 3945.00 3832.00 H 851782.00 15986654.00 18785548.00 22204608.00 14116305.00 16994480.00 13181629.00 3907.00 3851.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 75136136.50 63559092.50 18568358.50 29838702.50 8555481.50 25736304.50 31587670.50 -48044.50 -48195.50 B 31386734.50 28510464.50 17254054.50 8852572.50 12743077.50 38111932.50 17987564.50 -47874.50 -48063.50 C 19640082.50 11419413.50 5392015.50 23203360.50 35758764.50 6178535.50 3453076.50 -47988.50 -48139.50 D 9485509.50 59965708.50 10861852.50 112071656.50 4343826.50 3771457.50 9431340.50 -47893.50 -48328.50 E 6296257.50 24439004.50 9723184.50 5523608.50 47160760.50 2689313.50 34427348.50 -47912.50 -48025.50 F 2001880.50 8611568.50 13826776.50 20112518.50 10464276.50 20688422.50 10961916.50 -47856.50 -47988.50 G 1291699.50 24082790.50 9698838.50 18794134.50 9034021.50 6802485.50 11382655.50 -47950.50 -48063.50 H 799886.50 15934758.50 18733652.50 22152712.50 14064409.50 16942584.50 13129733.50 -47988.50 -48044.50 Load time: 03/06/2013 11:37:12 AM Read time: 03/06/2013 11:37:12 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2967 Comment: plate2-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 99505696 cps Min counts: 1736 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 103131.00 71134736.00 58635424.00 34896736.00 99505696.00 49957608.00 22685432.00 84113696.00 3983.00 3700.00 B 36397.00 37507584.00 78461008.00 16336359.00 78328272.00 28951740.00 28368088.00 7405797.00 4134.00 3530.00 C 45590.00 22476662.00 17284210.00 24059596.00 42236956.00 38531624.00 11771289.00 25504950.00 3870.00 3605.00 D 46553.00 10851214.00 9810923.00 22435966.00 27494956.00 32692608.00 44387552.00 8371891.00 3888.00 3643.00 E 6441186.00 3075653.00 54432292.00 17921474.00 48751892.00 52051352.00 45779288.00 3718.00 3643.00 F 2139761.00 16283311.00 36253572.00 42782860.00 27891458.00 30716782.00 59180680.00 3737.00 3643.00 G 1382654.00 11409344.00 25295942.00 57099632.00 52951732.00 79181696.00 39460528.00 3681.00 3662.00 H 623167.00 17441324.00 16940754.00 42823140.00 15466153.00 76398176.00 39620464.00 3662.00 3473.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71076818.25 58577506.25 34838818.25 99447778.25 49899690.25 22627514.25 84055778.25 -53934.75 -54217.75 B 37449666.25 78403090.25 16278441.25 78270354.25 28893822.25 28310170.25 7347879.25 -53783.75 -54387.75 C 22418744.25 17226292.25 24001678.25 42179038.25 38473706.25 11713371.25 25447032.25 -54047.75 -54312.75 D 10793296.25 9753005.25 22378048.25 27437038.25 32634690.25 44329634.25 8313973.25 -54029.75 -54274.75 E 6383268.25 3017735.25 54374374.25 17863556.25 48693974.25 51993434.25 45721370.25 -54199.75 -54274.75 F 2081843.25 16225393.25 36195654.25 42724942.25 27833540.25 30658864.25 59122762.25 -54180.75 -54274.75 G 1324736.25 11351426.25 25238024.25 57041714.25 52893814.25 79123778.25 39402610.25 -54236.75 -54255.75 H 565249.25 17383406.25 16882836.25 42765222.25 15408235.25 76340258.25 39562546.25 -54255.75 -54444.75 Load time: 03/06/2013 11:37:12 AM Read time: 03/06/2013 11:37:12 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2967 Comment: plate2-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 99505696 cps Min counts: 1736 counts Well list: r0(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 103131.00 71134736.00 58635424.00 34896736.00 99505696.00 49957608.00 22685432.00 84113696.00 3983.00 3700.00 B 36397.00 37507584.00 78461008.00 16336359.00 78328272.00 28951740.00 28368088.00 7405797.00 4134.00 3530.00 C 45590.00 22476662.00 17284210.00 24059596.00 42236956.00 38531624.00 11771289.00 25504950.00 3870.00 3605.00 D 46553.00 10851214.00 9810923.00 22435966.00 27494956.00 32692608.00 44387552.00 8371891.00 3888.00 3643.00 E 6441186.00 3075653.00 54432292.00 17921474.00 48751892.00 52051352.00 45779288.00 3718.00 3643.00 F 2139761.00 16283311.00 36253572.00 42782860.00 27891458.00 30716782.00 59180680.00 3737.00 3643.00 G 1382654.00 11409344.00 25295942.00 57099632.00 52951732.00 79181696.00 39460528.00 3681.00 3662.00 H 623167.00 17441324.00 16940754.00 42823140.00 15466153.00 76398176.00 39620464.00 3662.00 3473.00 Well list: r1(a2:h2,a3:h3,a4:h4,a5:h10) = cps(f2(a2:h2,a3:h3,a4:h4,a5:h10) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71076818.25 58577506.25 34838818.25 99447778.25 49899690.25 22627514.25 84055778.25 -53934.75 -54217.75 B 37449666.25 78403090.25 16278441.25 78270354.25 28893822.25 28310170.25 7347879.25 -53783.75 -54387.75 C 22418744.25 17226292.25 24001678.25 42179038.25 38473706.25 11713371.25 25447032.25 -54047.75 -54312.75 D 10793296.25 9753005.25 22378048.25 27437038.25 32634690.25 44329634.25 8313973.25 -54029.75 -54274.75 E 6383268.25 3017735.25 54374374.25 17863556.25 48693974.25 51993434.25 45721370.25 -54199.75 -54274.75 F 2081843.25 16225393.25 36195654.25 42724942.25 27833540.25 30658864.25 59122762.25 -54180.75 -54274.75 G 1324736.25 11351426.25 25238024.25 57041714.25 52893814.25 79123778.25 39402610.25 -54236.75 -54255.75 H 565249.25 17383406.25 16882836.25 42765222.25 15408235.25 76340258.25 39562546.25 -54255.75 -54444.75 Load time: 03/06/2013 06:07:33 PM Read time: 03/06/2013 06:07:33 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2969 Comment: plate1-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 9 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 90349960 cps Min counts: 29267 counts Well list: r0(a2:h8,a1:c1) = cps(f2(a2:h8,a1:c1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35561.00 72621400.00 64886456.00 20028346.00 19956918.00 8805101.00 16520625.00 40081092.00 B 40584.00 43353044.00 30275332.00 18364322.00 9049661.00 12871267.00 24249436.00 18934502.00 C 32519.00 20940836.00 11319309.00 14935047.00 21574068.00 31966386.00 6228347.00 3633134.00 D 10385843.00 90349960.00 31675582.00 43363176.00 4528248.00 2961490.00 21159434.00 E 6348192.00 33808420.00 10455889.00 5904490.00 51667408.00 2706496.00 63260172.00 F 2342661.00 8868482.00 48068872.00 20866236.00 18789786.00 21737602.00 33954672.00 G 1593868.00 26583002.00 10593739.00 20910850.00 15841072.00 7197842.00 23205954.00 H 691098.00 18268718.00 56343952.00 19362006.00 28016696.00 21664478.00 13415200.00 Well list: r1(a2:h8) = cps(f2(a2:h8) - avg(f2(a1:c1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 72585178.67 64850234.67 19992124.67 19920696.67 8768879.67 16484403.67 40044870.67 B 43316822.67 30239110.67 18328100.67 9013439.67 12835045.67 24213214.67 18898280.67 C 20904614.67 11283087.67 14898825.67 21537846.67 31930164.67 6192125.67 3596912.67 D 10349621.67 90313738.67 31639360.67 43326954.67 4492026.67 2925268.67 21123212.67 E 6311970.67 33772198.67 10419667.67 5868268.67 51631186.67 2670274.67 63223950.67 F 2306439.67 8832260.67 48032650.67 20830014.67 18753564.67 21701380.67 33918450.67 G 1557646.67 26546780.67 10557517.67 20874628.67 15804850.67 7161620.67 23169732.67 H 654876.67 18232496.67 56307730.67 19325784.67 27980474.67 21628256.67 13378978.67 Load time: 03/06/2013 06:08:58 PM Read time: 03/06/2013 06:08:58 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2970 Comment: plate2-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 9 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 86936544 cps Min counts: 32750 counts Well list: r0(a2:h8,a1:c1) = cps(f2(a2:h8,a1:c1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 43793.00 71872816.00 50929452.00 21539576.00 53873876.00 49659904.00 58384040.00 15922685.00 B 236962.00 30488900.00 76127848.00 20659876.00 20548538.00 43149540.00 42747168.00 30372058.00 C 36389.00 21895498.00 19811578.00 17677418.00 44542004.00 20111032.00 43384396.00 9343207.00 D 11110378.00 11063955.00 20722448.00 42693660.00 31323680.00 33127050.00 45723228.00 E 7003244.00 3386807.00 56367448.00 86936544.00 19288054.00 84679456.00 49778912.00 F 2262468.00 26612382.00 49190724.00 47436288.00 44809180.00 79966656.00 21556492.00 G 1655297.00 12487101.00 34689284.00 33135492.00 49521720.00 61924840.00 57834508.00 H 707384.00 36415680.00 25643448.00 27825238.00 39925748.00 17477358.00 60640232.00 Well list: r1(a2:h8) = cps(f2(a2:h8) - avg(f2(a1:c1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71767101.33 50823737.33 21433861.33 53768161.33 49554189.33 58278325.33 15816970.33 B 30383185.33 76022133.33 20554161.33 20442823.33 43043825.33 42641453.33 30266343.33 C 21789783.33 19705863.33 17571703.33 44436289.33 20005317.33 43278681.33 9237492.33 D 11004663.33 10958240.33 20616733.33 42587945.33 31217965.33 33021335.33 45617513.33 E 6897529.33 3281092.33 56261733.33 86830829.33 19182339.33 84573741.33 49673197.33 F 2156753.33 26506667.33 49085009.33 47330573.33 44703465.33 79860941.33 21450777.33 G 1549582.33 12381386.33 34583569.33 33029777.33 49416005.33 61819125.33 57728793.33 H 601669.33 36309965.33 25537733.33 27719523.33 39820033.33 17371643.33 60534517.33 Load time: 03/06/2013 06:08:58 PM Read time: 03/06/2013 06:08:58 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2970 Comment: plate2-1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 9 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 86936544 cps Min counts: 32750 counts Well list: r0(a2:h8,a1:c1) = cps(f2(a2:h8,a1:c1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 43793.00 71872816.00 50929452.00 21539576.00 53873876.00 49659904.00 58384040.00 15922685.00 B 236962.00 30488900.00 76127848.00 20659876.00 20548538.00 43149540.00 42747168.00 30372058.00 C 36389.00 21895498.00 19811578.00 17677418.00 44542004.00 20111032.00 43384396.00 9343207.00 D 11110378.00 11063955.00 20722448.00 42693660.00 31323680.00 33127050.00 45723228.00 E 7003244.00 3386807.00 56367448.00 86936544.00 19288054.00 84679456.00 49778912.00 F 2262468.00 26612382.00 49190724.00 47436288.00 44809180.00 79966656.00 21556492.00 G 1655297.00 12487101.00 34689284.00 33135492.00 49521720.00 61924840.00 57834508.00 H 707384.00 36415680.00 25643448.00 27825238.00 39925748.00 17477358.00 60640232.00 Well list: r1(a2:h8) = cps(f2(a2:h8) - avg(f2(a1:c1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71767101.33 50823737.33 21433861.33 53768161.33 49554189.33 58278325.33 15816970.33 B 30383185.33 76022133.33 20554161.33 20442823.33 43043825.33 42641453.33 30266343.33 C 21789783.33 19705863.33 17571703.33 44436289.33 20005317.33 43278681.33 9237492.33 D 11004663.33 10958240.33 20616733.33 42587945.33 31217965.33 33021335.33 45617513.33 E 6897529.33 3281092.33 56261733.33 86830829.33 19182339.33 84573741.33 49673197.33 F 2156753.33 26506667.33 49085009.33 47330573.33 44703465.33 79860941.33 21450777.33 G 1549582.33 12381386.33 34583569.33 33029777.33 49416005.33 61819125.33 57728793.33 H 601669.33 36309965.33 25537733.33 27719523.33 39820033.33 17371643.33 60534517.33