Load time: 04/12/2013 01:03:06 PM Read time: 04/12/2013 01:03:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3010 Comment: std+syn Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 81219096 cps Min counts: 66865 counts Well list: r0(a1:h3,a4:h12) = cps(f2(a1:h3,a4:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 522599.00 516711.00 560703.00 2157991.00 1926548.00 2212390.00 8689474.00 11013487.00 3379576.00 3643794.00 2687004.00 133730.00 B 773056.00 841545.00 822653.00 3624665.00 3224081.00 3642743.00 6781374.00 9096235.00 4858097.00 3859410.00 1707251.00 139184.00 C 1325909.00 1376581.00 1461206.00 6480622.00 5165872.00 5676350.00 3604777.00 12605222.00 5757970.00 4915228.00 2490533.00 137241.00 D 2221940.00 2228773.00 2639925.00 9750222.00 9064949.00 10000427.00 11227451.00 9224196.00 7327590.00 7697069.00 4627731.00 139732.00 E 3700939.00 3492173.00 4358700.00 17580560.00 17409084.00 15869105.00 9986177.00 10261142.00 6789164.00 11032302.00 2207308.00 136618.00 F 6627545.00 6883487.00 8016865.00 29017994.00 28218242.00 28640304.00 8976267.00 13747277.00 9474933.00 10570751.00 2098009.00 301413.00 G 10855652.00 10408858.00 12796315.00 51644428.00 51579304.00 49172668.00 7088399.00 8742334.00 10090762.00 11041646.00 3251558.00 144601.00 H 19978008.00 21631502.00 22326298.00 79840840.00 81219096.00 78830416.00 4777039.00 3232889.00 5662616.00 7319826.00 6169291.00 136278.00 Load time: 04/12/2013 01:06:02 PM Read time: 04/12/2013 01:06:02 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3011 Comment: syn Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 15806220 cps Min counts: 65742 counts Well list: r0(a1:h3,a4:h12) = cps(f2(a1:h3,a4:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7696939.00 8147187.00 8245228.00 2918735.00 7270662.00 7216477.00 7269760.00 4116366.00 6075153.00 7137136.00 6915403.00 15806220.00 B 7700834.00 3590822.00 5040804.00 7928310.00 10050198.00 3769555.00 10612051.00 6317817.00 4435164.00 5692108.00 10282024.00 5613371.00 C 7209922.00 13527657.00 8640327.00 6271440.00 3727687.00 3000249.00 5251394.00 1842118.00 5301695.00 8670895.00 5849681.00 8029765.00 D 4801298.00 7325820.00 12336800.00 9577509.00 7568400.00 4448037.00 8994837.00 7360733.00 1738075.00 3763660.00 9907959.00 1454166.00 E 7212956.00 7553557.00 5339362.00 5302124.00 5035167.00 3798475.00 7490501.00 4854323.00 5456076.00 5888246.00 10280984.00 12557979.00 F 4207341.00 6977235.00 6232587.00 5345343.00 5324893.00 5773158.00 7548550.00 10133063.00 3910763.00 4809901.00 8705542.00 7964530.00 G 4985206.00 6587002.00 10766581.00 6072200.00 5658226.00 9516856.00 8268070.00 8201253.00 2775040.00 6530840.00 12086221.00 9857296.00 H 131484.00 5681222.00 8592222.00 3061635.00 9395917.00 10925647.00 11931387.00 3374742.00 2376555.00 8179723.00 9414605.00 3236897.00 Load time: 04/12/2013 01:08:20 PM Read time: 04/12/2013 01:08:20 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3012 Comment: FGI plate 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 65490104 cps Min counts: 773019 counts Well list: r0(a1:h3,a4:h12) = cps(f2(a1:h3,a4:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 44302380.00 38110540.00 25840602.00 33870472.00 27615612.00 18392384.00 43625576.00 27915088.00 32135750.00 17690678.00 27477696.00 24059182.00 B 46997184.00 48258492.00 24386538.00 22865986.00 28839042.00 31176530.00 36209860.00 19857610.00 24158808.00 27990896.00 25084418.00 21831330.00 C 47414300.00 55363540.00 24688180.00 23184194.00 31773562.00 42639680.00 33181910.00 24303424.00 28283908.00 23237930.00 28856162.00 35365980.00 D 51119172.00 56926276.00 33086752.00 42002316.00 29751984.00 35566284.00 50901052.00 14321876.00 23726356.00 33735072.00 25332344.00 23120882.00 E 63810984.00 58738260.00 42898072.00 29071686.00 30072710.00 39128116.00 47379136.00 18468580.00 30530580.00 23086426.00 24825742.00 26037922.00 F 65006016.00 33550880.00 31961556.00 28857992.00 34256368.00 47299700.00 55613680.00 19541704.00 30128150.00 19235024.00 27799736.00 31680586.00 G 65490104.00 30259528.00 19148304.00 30833440.00 29654628.00 40611040.00 60769548.00 20381814.00 30298668.00 20010952.00 33946328.00 32527486.00 H 45595624.00 58567816.00 30075166.00 40129524.00 21510036.00 29222468.00 42374856.00 15129676.00 29237610.00 21892974.00 18331496.00 28436648.00 Load time: 04/12/2013 01:12:07 PM Read time: 04/12/2013 01:12:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3013 Comment: FGI plate 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27874724 cps Min counts: 287579 counts Well list: r0(a1:h3,a4:h12) = cps(f2(a1:h3,a4:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14226279.00 13646200.00 12156815.00 14895456.00 2760933.00 6050721.00 3392524.00 1608957.00 1983036.00 746465.00 8558081.00 9396546.00 B 14592395.00 5646975.00 9136606.00 8271900.00 4694076.00 5469108.00 6113098.00 7850299.00 13755963.00 2475184.00 16821510.00 11078184.00 C 15692121.00 6359888.00 18225948.00 6653421.00 4668522.00 7765726.00 13004834.00 9409722.00 7930702.00 3853006.00 16938500.00 10840930.00 D 12236019.00 15873080.00 19891692.00 9190222.00 3766374.00 7560812.00 4344522.00 4927872.00 10575496.00 1407759.00 12019451.00 7016086.00 E 14227509.00 16791802.00 6654342.00 18634106.00 4819993.00 8581997.00 7695429.00 10291181.00 15347057.00 594428.00 20525336.00 6484536.00 F 7350975.00 27874724.00 3223553.00 15302519.00 9309613.00 1661422.00 16000262.00 3910913.00 7502249.00 819275.00 23830956.00 12971577.00 G 21196488.00 5860102.00 5268359.00 4408638.00 3119682.00 4088206.00 2552720.00 3007555.00 18334584.00 647063.00 1455978.00 8629332.00 H 12258877.00 2823931.00 4081961.00 4890142.00 9417299.00 11838162.00 2692787.00 2350988.00 1266592.00 575159.00 1687133.00 12939917.00 Load time: 04/12/2013 01:14:24 PM Read time: 04/12/2013 01:14:24 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3014 Comment: FGI plate 3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25421148 cps Min counts: 66893 counts Well list: r0(a1:h3,a4:h12) = cps(f2(a1:h3,a4:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6670150.00 133787.00 5526487.00 7948275.00 8772563.00 6680271.00 1760201.00 3467326.00 13762550.00 7088067.00 13666368.00 11748225.00 B 4676939.00 5453242.00 20010588.00 4656110.00 12995301.00 6158925.00 4028717.00 12878821.00 1744333.00 13592642.00 3763741.00 15582422.00 C 3903681.00 7999212.00 12668601.00 8954462.00 10849846.00 7918775.00 7798505.00 5204810.00 9981098.00 6674682.00 5995584.00 18812644.00 D 5708716.00 9380627.00 10546206.00 10669189.00 8141798.00 11563677.00 2001272.00 9376007.00 18819468.00 7743439.00 12013867.00 12770946.00 E 6303849.00 6603534.00 10207755.00 8564661.00 7820644.00 9067428.00 950987.00 4784211.00 25421148.00 6638114.00 6639762.00 14141489.00 F 10521832.00 19329534.00 9110558.00 9887252.00 14777286.00 6129312.00 1156282.00 8345306.00 7625236.00 10748467.00 14647226.00 12834601.00 G 3139288.00 22317786.00 5553453.00 20289690.00 18195148.00 9631867.00 1066279.00 4037770.00 6587682.00 10405621.00 6033516.00 6994723.00 H 3965709.00 4324279.00 6888136.00 6977197.00 4413249.00 10702232.00 1030270.00 14120849.00 16188134.00 16273674.00 8485046.00 13451272.00 Load time: 04/12/2013 01:16:37 PM Read time: 04/12/2013 01:16:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3015 Comment: FGI plate 4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 65329600 cps Min counts: 773019 counts Well list: r0(a1:h3,a4:h12) = cps(f2(a1:h3,a4:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9401562.00 14970311.00 18522258.00 43009540.00 27842796.00 35592656.00 43672156.00 37324796.00 49687228.00 39007476.00 30475934.00 31930666.00 B 10418258.00 26647648.00 38508468.00 33013552.00 33768084.00 21668064.00 29249780.00 39423856.00 43129520.00 37522644.00 32432636.00 28849268.00 C 6487948.00 33679368.00 33017078.00 41336808.00 48944148.00 28178436.00 24598386.00 36699632.00 40460760.00 38306384.00 33862604.00 53037324.00 D 11747992.00 38852592.00 27999618.00 48917828.00 61927724.00 31004722.00 25747990.00 38010472.00 39986500.00 30615826.00 49903420.00 26800644.00 E 10030715.00 40865236.00 38378844.00 46071452.00 51255428.00 32720934.00 32349036.00 37424368.00 43104748.00 65329600.00 50673488.00 47377680.00 F 37159908.00 25710566.00 41720088.00 26357984.00 47859620.00 41097704.00 23231224.00 45958868.00 29583244.00 44083592.00 42102056.00 57486352.00 G 14097363.00 38981908.00 8196992.00 33932024.00 42201580.00 29787638.00 28266532.00 30392656.00 60492304.00 28614118.00 36934424.00 31687342.00 H 12812350.00 19763738.00 31723622.00 38932432.00 34162356.00 39142184.00 28798754.00 37285196.00 22896600.00 24049224.00 26262076.00 22701134.00 Load time: 04/12/2013 01:18:25 PM Read time: 04/12/2013 01:18:25 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3016 Comment: FGI plate 5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 82167880 cps Min counts: 95825 counts Well list: r0(a1:h3,a4:h12) = cps(f2(a1:h3,a4:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 32386304.00 23484670.00 53238956.00 60337432.00 26965278.00 42972956.00 28590730.00 25185268.00 42572284.00 51295564.00 1712576.00 2589283.00 B 31895880.00 31647124.00 54453680.00 41387492.00 55315612.00 51247956.00 27721280.00 22834714.00 44040896.00 45796860.00 13129924.00 794194.00 C 41006140.00 39495360.00 51005508.00 191650.00 35886772.00 66832576.00 46145156.00 49179236.00 47924304.00 44383784.00 7878230.00 930019.00 D 22165166.00 42481740.00 79130600.00 46459940.00 82167880.00 41374864.00 59141360.00 40758792.00 45160632.00 42867624.00 5085116.00 2005396.00 E 49780340.00 23432772.00 54289620.00 55526200.00 45650008.00 42616408.00 38762000.00 32710966.00 50662864.00 54039888.00 7028263.00 1029893.00 F 12499943.00 24896022.00 52087776.00 46117352.00 60475740.00 41835920.00 35971268.00 57604160.00 55190024.00 51390024.00 7378142.00 1069072.00 G 42682528.00 20887550.00 56340032.00 43178908.00 52550936.00 67049960.00 42965076.00 51181112.00 41877620.00 44493628.00 14373459.00 4695461.00 H 21488210.00 8246582.00 65304212.00 29899164.00 47821424.00 80328680.00 32918098.00 29744430.00 60333192.00 72578496.00 1152847.00 1185837.00 Load time: 04/12/2013 01:20:09 PM Read time: 04/12/2013 01:20:09 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3017 Comment: FGI plate 6 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 88483568 cps Min counts: 72338 counts Well list: r0(a1:h3,a4:h12) = cps(f2(a1:h3,a4:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26874998.00 19213364.00 27067534.00 17693270.00 21983190.00 16348185.00 32039450.00 24957754.00 32960770.00 39476404.00 27914684.00 29987082.00 B 57638948.00 31150904.00 20314006.00 34408240.00 19222300.00 45386808.00 58974424.00 32293762.00 42818472.00 19358044.00 46577512.00 17919426.00 C 29734130.00 25453878.00 26641772.00 22433138.00 33063686.00 18992536.00 34235128.00 40800096.00 68531872.00 39229384.00 36169032.00 55183324.00 D 23514888.00 46569372.00 27148908.00 49278936.00 29930074.00 12999540.00 54547056.00 34697828.00 60746148.00 28934792.00 27374746.00 27443844.00 E 22047266.00 25367008.00 34243020.00 36483124.00 24071842.00 25251414.00 20664870.00 20266606.00 62870076.00 47028920.00 32515450.00 17906434.00 F 24051992.00 47123236.00 31460750.00 20662880.00 18915388.00 32017158.00 19055740.00 26556014.00 42243092.00 33126878.00 26843174.00 34489760.00 G 88483568.00 18406702.00 26750376.00 13233704.00 28366110.00 29009390.00 19131434.00 22021516.00 21416712.00 27205854.00 144676.00 28553980.00 H 37498800.00 40385720.00 39677816.00 30831596.00 48188396.00 22395768.00 13863997.00 20797132.00 29965694.00 39621680.00 26237388.00 10968745.00 Load time: 04/12/2013 01:21:50 PM Read time: 04/12/2013 01:21:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3018 Comment: FGI plate 7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 161919312 cps Min counts: 773019 counts Well list: r0(a1:h3,a4:h12) = cps(f2(a1:h3,a4:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 30815004.00 71200712.00 35657008.00 28426506.00 32478426.00 62539464.00 33549864.00 24946800.00 25188468.00 45472776.00 45773812.00 42969848.00 B 23687462.00 29942362.00 53548748.00 46407780.00 43379292.00 40097052.00 161919312.00 47128124.00 58238208.00 68589040.00 39426668.00 36909204.00 C 31070020.00 34871728.00 37117800.00 55865276.00 27281708.00 57038424.00 55145532.00 48081092.00 82030120.00 43307844.00 28405406.00 46750472.00 D 51540788.00 56813312.00 35551892.00 41900320.00 60269216.00 57788468.00 47952844.00 46226284.00 85192424.00 61131896.00 68838848.00 43712908.00 E 31588416.00 3909878.00 32465468.00 47390172.00 56175848.00 56970328.00 47457376.00 61438484.00 100001160.00 59707804.00 39825400.00 75035816.00 F 44645760.00 34625712.00 54506284.00 42788372.00 49000928.00 58722640.00 32241232.00 63785712.00 74136992.00 68051648.00 31537508.00 80138816.00 G 23912560.00 26138642.00 45138740.00 66699948.00 45586204.00 55586884.00 37454652.00 80039248.00 70523216.00 68067216.00 50775340.00 56472564.00 H 33040932.00 34423104.00 38744716.00 36973112.00 36171568.00 35993880.00 59783068.00 60341200.00 57397144.00 32799236.00 36430512.00 49220896.00 Load time: 04/12/2013 01:27:11 PM Read time: 04/12/2013 01:27:11 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3019 Comment: std 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 286123200 cps Min counts: 61967 counts Well list: r0(a1:h3,a4:h12) = cps(f2(a1:h3,a4:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 454847.00 445901.00 485420.00 6565597.00 6667900.00 1960604.00 8254340.00 10419548.00 3272843.00 3455505.00 2653997.00 123935.00 B 680826.00 730272.00 715250.00 11233292.00 11663916.00 3223367.00 6529886.00 8717794.00 4584567.00 3645657.00 1619683.00 131598.00 C 1140995.00 1177212.00 1261497.00 19156754.00 17764386.00 4796768.00 3446984.00 11923069.00 5489751.00 4687079.00 2408045.00 126691.00 D 1877207.00 1877904.00 2229223.00 30297718.00 30293326.00 9080038.00 10761964.00 8785115.00 7001153.00 7373483.00 4437504.00 132957.00 E 3064557.00 2970912.00 3610515.00 52228192.00 54380512.00 13255966.00 9470314.00 9707159.00 6449822.00 10423567.00 2280246.00 129352.00 F 5445456.00 5780788.00 6665038.00 88531208.00 86670072.00 23054688.00 8650326.00 13160145.00 8942271.00 10133701.00 1913785.00 290486.00 G 9180476.00 8648787.00 10827720.00 166079920.00 162187696.00 39884580.00 6874904.00 8373338.00 8690333.00 10595471.00 3119898.00 132824.00 H 16943326.00 18380750.00 18067904.00 283759968.00 286123200.00 65216612.00 4551842.00 3100636.00 5432456.00 7008148.00 5870008.00 126672.00