Load time: 03/22/2013 03:32:41 PM Read time: 03/22/2013 03:32:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2990 Comment: plate1_2mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 2 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 123004984 cps Min counts: 23930 counts Well list: r0(a2:h8,a1:d1) = cps(f2(a2:h8,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 47861.00 123004984.00 22441084.00 3166212.00 1724870.00 7791564.00 7901539.00 12664339.00 B 49068.00 76917616.00 1149468.00 4808809.00 18057160.00 3245662.00 11083512.00 3251207.00 C 274968.00 48211300.00 823433.00 1299577.00 7954233.00 221807.00 2198317.00 3809604.00 D 51727.00 29038862.00 436392.00 9236903.00 11510307.00 1538979.00 1428019.00 1827525.00 E 16433674.00 2584613.00 2538599.00 5004741.00 1689482.00 6209151.00 991231.00 F 9401346.00 13817235.00 5331322.00 4535709.00 22336734.00 4141136.00 12375368.00 G 5663670.00 2198806.00 19952576.00 1284774.00 775929.00 23329672.00 545108.00 H 3973363.00 13780554.00 4326412.00 1885068.00 8540184.00 2797906.00 930282.00 Well list: r1(a2:h8) = cps(f2(a2:h8) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 122899078.00 22335178.00 3060306.00 1618964.00 7685658.00 7795633.00 12558433.00 B 76811710.00 1043562.00 4702903.00 17951254.00 3139756.00 10977606.00 3145301.00 C 48105394.00 717527.00 1193671.00 7848327.00 115901.00 2092411.00 3703698.00 D 28932956.00 330486.00 9130997.00 11404401.00 1433073.00 1322113.00 1721619.00 E 16327768.00 2478707.00 2432693.00 4898835.00 1583576.00 6103245.00 885325.00 F 9295440.00 13711329.00 5225416.00 4429803.00 22230828.00 4035230.00 12269462.00 G 5557764.00 2092900.00 19846670.00 1178868.00 670023.00 23223766.00 439202.00 H 3867457.00 13674648.00 4220506.00 1779162.00 8434278.00 2692000.00 824376.00 Load time: 03/22/2013 03:35:07 PM Read time: 03/22/2013 03:35:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2991 Comment: plate2_1mm Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 1 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 149119120 cps Min counts: 26806 counts Well list: r0(a2:h8,a1:d1) = cps(f2(a2:h8,a1:d1)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71565.00 149119120.00 8140150.00 26475116.00 438108.00 11742490.00 14510212.00 13391669.00 B 54178.00 81944160.00 32459892.00 20799094.00 29140856.00 15117787.00 1041563.00 8563154.00 C 53613.00 55923580.00 3336115.00 3445415.00 8331019.00 4616183.00 3079558.00 1135871.00 D 53726.00 33152730.00 3707338.00 5680965.00 8373439.00 746058.00 11416889.00 8166840.00 E 21698104.00 195481.00 6291102.00 5856021.00 5317189.00 1312910.00 963774.00 F 11379348.00 4332413.00 675398.00 1435166.00 3079799.00 2014889.00 2484297.00 G 6869675.00 12382132.00 1330429.00 3136034.00 8120902.00 9556854.00 598231.00 H 4516689.00 19485580.00 1788428.00 1395772.00 386248.00 12941039.00 14766920.00 Well list: r1(a2:h8) = cps(f2(a2:h8) - avg(f2(a1:d1))) Data: BACKGROUND SUBTRACTED DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 149060849.50 8081879.50 26416845.50 379837.50 11684219.50 14451941.50 13333398.50 B 81885889.50 32401621.50 20740823.50 29082585.50 15059516.50 983292.50 8504883.50 C 55865309.50 3277844.50 3387144.50 8272748.50 4557912.50 3021287.50 1077600.50 D 33094459.50 3649067.50 5622694.50 8315168.50 687787.50 11358618.50 8108569.50 E 21639833.50 137210.50 6232831.50 5797750.50 5258918.50 1254639.50 905503.50 F 11321077.50 4274142.50 617127.50 1376895.50 3021528.50 1956618.50 2426026.50 G 6811404.50 12323861.50 1272158.50 3077763.50 8062631.50 9498583.50 539960.50 H 4458418.50 19427309.50 1730157.50 1337501.50 327977.50 12882768.50 14708649.50