##BLOCKS= 18 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 25.5 717 160950 173740 130246 93251 19607 103073 300 47 4 2 4 251 1008 1788 768 560 605 1568 94 43 9 7 9 49 103 159 110 103 69 58 31 7 11 0 11 544 482 529 428 486 432 370 20 9 4 2 7 ~End Plate: Plate2 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 26.5 0 11 27 27 40 38 18 7 4 4 7 4 473 1600 2778 3661 5666 10249 31 7 7 4 11 7 9 13 22 20 29 58 22 20 7 0 20 7 ~End Plate: Plate3 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 26.5 16 7 13 31 27 54 16 4 7 9 260 235 553 1651 2800 3394 5581 10271 36 13 18 7 7 11 2 18 11 31 36 63 9 11 7 16 2 7 ~End Plate: Plate4 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 27 9 9 25 29 40 31 16 7 11 4 179 168 493 1618 2527 3547 5249 10083 29 11 4 7 4 4 2 11 22 16 38 25 27 13 16 11 13 4 ~End Plate: Plate5 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 27 9 13 31 34 27 43 9 7 4 11 421 426 466 1476 2572 3374 5270 9467 31 9 2 9 13 11 ~End Plate: Plate6 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 27 260 52787 58502 41516 30219 8671 73532 307 29 11 2 7 92 271 309 246 177 166 338 72 25 11 2 0 18 36 56 49 45 47 36 29 13 4 7 2 453 408 392 361 352 388 347 20 7 4 7 7 181 172 202 168 509 13 18 0 4 9 0 4 16 7 16 11 11 22 65 36 18 25 9 16 ~End Note: plate 3 standard ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 717.000 R 02 A2 160950.000 R 03 A3 173740.000 R 04 A4 130246.000 R 05 A5 93251.000 R 06 A6 19607.000 R 07 A7 103073.000 R Group Summaries InRange R - Outside standard range ~End Plate: RFUA 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 27.5 29 455 202 972 636 1013 4 7 2 4 4 4 52 314 190 260 52 134 323 172 4 9 2 7 128 29 159 175 60 83 455 63 4 0 2 7 20 34 116 432 125 148 365 141 4 2 4 29 2 7 9 9 4 0 4 2 7 0 0 25 ~End Plate: RFUA2 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 27.5 16 455 166 905 618 1082 7 2 0 4 0 4 43 305 224 224 60 134 325 235 9 4 7 7 152 18 199 164 99 134 493 85 0 2 2 2 38 54 121 482 141 195 376 175 9 0 0 16 0 2 7 2 4 4 4 4 0 0 4 29 4 0 0 4 0 4 7 2 4 7 0 22 4 2 2 2 0 2 0 0 2 0 2 16 ~End Plate: RFUB 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 27.5 16 446 235 847 652 1044 0 4 0 2 4 4 16 208 193 199 65 108 354 186 2 2 2 4 114 20 175 148 67 76 367 85 0 0 0 2 18 40 92 488 103 161 280 128 4 2 2 20 0 2 2 7 2 4 11 4 4 7 0 16 2 2 0 0 4 7 2 4 2 4 0 29 4 2 7 4 2 4 0 0 2 0 4 7 ~End Plate: RFUC 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 27.5 16 417 240 977 612 966 13 2 2 4 0 2 83 186 522 562 121 276 251 598 7 0 0 7 302 78 491 332 181 67 1898 202 2 2 0 2 63 101 63 2045 54 408 235 83 2 7 4 36 2 2 4 4 0 4 4 7 0 0 0 20 2 0 0 4 4 7 2 2 2 2 0 11 4 4 0 2 2 0 2 0 0 2 2 16 2 2 2 0 4 7 0 2 0 0 7 58 ~End Note: plate 3 standard ~End Plate: RFUA 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 27.5 0 11 20 54 110 99 2 2 4 0 7 2 2 13 2 20 13 13 31 45 7 4 0 0 7 7 7 40 18 16 34 36 0 2 2 4 4 9 4 38 13 9 22 25 2 2 0 2 4 4 2 7 0 0 2 2 2 4 2 7 ~End Plate: RFUA2 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 27.5 7 11 16 52 72 81 2 2 2 2 0 0 7 27 4 34 7 20 29 60 4 0 2 4 16 4 9 38 27 11 63 43 4 7 4 2 4 7 4 38 20 18 13 38 2 4 9 0 0 2 0 0 2 2 0 0 0 0 4 2 ~End Plate: RFUC 1.3 PlateFormat Endpoint Luminescence Raw FALSE 1 1 0 1 12 96 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 27.5 4 2 18 49 69 108 7 0 0 0 4 0 4 9 25 63 22 49 40 161 4 2 2 0 18 7 11 18 7 9 43 40 4 4 0 0 2 9 16 184 16 16 58 87 4 2 0 2 2 2 0 4 2 2 4 0 4 0 2 0 ~End Note: plate 3 standard ~End Original Filename: Elevated trial1; Date Last Saved: 6/25/2018 5:10:22 PM