##BLOCKS= 4 Plate: Plate1 1.3 TimeFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 37 6455 8775902 8406496 10423063 16732 18694 18887 16281 126548 96719 96769 6024 6121 2527471 3215091 2251687 25611 71372 41145 50257 1537 1377 1517 1541 7537 1339720 1725292 1530766 19406 19636 12796 8177 1499 1459 1540 1424 9393 1067447 743293 840582 16784 16926 12248 15906 1643 1424 1380 1516 6630 429552 435322 355629 15714 29612 14608 15831 1471 1510 1379 1422 6355 343628 317021 196659 45712 29391 33626 16576 1443 1433 1465 1470 11664 215626 217386 217376 18891 49611 14435 12882 1465 1759 1519 1556 7038 66685 82625 49728 12193 24961 8976 18023 1479 1438 1401 1414 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 6455 8775902 8406496 10423063 16732 18694 18887 16281 126548 96719 96769 6024 6121 2527471 3215091 2251687 25611 71372 41145 50257 1537 1377 1517 1541 7537 1339720 1725292 1530766 19406 19636 12796 8177 1499 1459 1540 1424 9393 1067447 743293 840582 16784 16926 12248 15906 1643 1424 1380 1516 6630 429552 435322 355629 15714 29612 14608 15831 1471 1510 1379 1422 6355 343628 317021 196659 45712 29391 33626 16576 1443 1433 1465 1470 11664 215626 217386 217376 18891 49611 14435 12882 1465 1759 1519 1556 7038 66685 82625 49728 12193 24961 8976 18023 1479 1438 1401 1414 ~End Group: Blank Sample Well Concentration Values MeanValue Std.Dev. CV% 01 A1 0.000 6455.000 7649.125 1928.021 25.206 B1 6121.000 C1 7537.000 D1 9393.000 E1 6630.000 F1 6355.000 G1 11664.000 H1 7038.000 Group Summaries ~End Group: Libraries Sample Well Values Result MeanResult Std.Dev. CV% 01 A5 16732.000 -0.001 -0.001 0.000 11.636 A6 18694.000 -0.001 A7 18887.000 -0.001 A8 16281.000 -0.001 B5 25611.000 -0.001 B6 71372.000 -0.001 B7 41145.000 -0.001 B8 50257.000 -0.001 C5 19406.000 -0.001 C6 19636.000 -0.001 C7 12796.000 -0.001 C8 8177.000 -0.001 D5 16784.000 -0.001 D6 16926.000 -0.001 D7 12248.000 -0.001 D8 15906.000 -0.001 E5 15714.000 -0.001 E6 29612.000 -0.001 E7 14608.000 -0.001 E8 15831.000 -0.001 F5 45712.000 -0.001 F6 29391.000 -0.001 F7 33626.000 -0.001 F8 16576.000 -0.001 G5 18891.000 -0.001 G6 49611.000 -0.001 G7 14435.000 -0.001 G8 12882.000 -0.001 H5 12193.000 -0.001 H6 24961.000 -0.001 H7 8976.000 -0.001 H8 18023.000 -0.001 Group Summaries ~End Group: Standards Sample Concentration mg/ml BackCalcConc Wells Value MeanValue SD CV 01 0.100 0.094 A2 8775902.000 9201820.333 1073634.219 11.7 0.090 A3 8406496.000 0.112 A4 10423063.000 04 0.022 0.026 B2 2527471.000 2664749.667 496156.116 18.6 0.033 B3 3215091.000 0.023 B4 2251687.000 05 0.013 0.013 C2 1339720.000 1531926.000 192788.617 12.6 0.017 C3 1725292.000 0.015 C4 1530766.000 06 0.008 0.010 D2 1067447.000 883774.000 166337.355 18.8 0.007 D3 743293.000 0.008 D4 840582.000 07 0.005 0.003 E2 429552.000 406834.333 44438.866 10.9 0.003 E3 435322.000 0.002 E4 355629.000 08 0.003 0.002 F2 343628.000 285769.333 78310.100 27.4 0.002 F3 317021.000 0.001 F4 196659.000 09 0.002 0.001 G2 215626.000 216796.000 1013.262 0.5 0.001 G3 217386.000 0.001 G4 217376.000 10 0.001 -0.001 H2 66685.000 66346.000 16451.120 24.8 -0.001 H3 82625.000 -0.001 H4 49728.000 11 0.001 -0.000 A9 126548.000 106678.667 17207.366 16.1 -0.001 A10 96719.000 -0.001 A11 96769.000 Group Summaries MinStd Smallest standard value: 66346.000 Min(MeanValue) MaxStd Largest standard value: 9201820.333 Max(MeanValue) ~End Original Filename: Don_4_20_17; Date Last Saved: 4/20/2017 4:16:37 PM