Load time: 05/21/2013 01:57:30 PM Read time: 05/21/2013 01:57:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3037 Comment: Standards+extralibraries Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 87532728 cps Min counts: 1123 counts Well list: r0(a1:h9) = cps(f2(a1:h9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2340.00 74271968.00 82706624.00 87532728.00 310351.00 4647872.00 20549432.00 7361149.00 125050.00 B 2246.00 57484784.00 55851132.00 60942068.00 291589.00 7499872.00 11907475.00 5039024.00 134827.00 C 2340.00 30803312.00 29900436.00 28760246.00 157554.00 10323627.00 15897728.00 6253860.00 1960570.00 D 2265.00 15848293.00 15806594.00 13532880.00 192756.00 9818980.00 8263617.00 5068162.00 1737261.00 E 2283.00 8116213.00 7797122.00 7119318.00 144567.00 8769526.00 9802161.00 5263319.00 128429.00 F 2397.00 3975110.00 3631748.00 3535176.00 134356.00 16031136.00 24315456.00 4068142.00 118443.00 G 2378.00 2150492.00 1985836.00 1907648.00 140131.00 3670126.00 8126937.00 4101697.00 124823.00 H 2321.00 1194500.00 1099707.00 1255883.00 145228.00 8169841.00 12248758.00 6418147.00 134903.00 Load time: 05/21/2013 01:59:57 PM Read time: 05/21/2013 01:59:57 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3038 Comment: BioRep2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 20109544 cps Min counts: 187868 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 933527.00 5288948.00 2938732.00 7374040.00 3009067.00 3604771.00 5697037.00 3283855.00 12592211.00 9535739.00 B 375736.00 2890744.00 1857237.00 8783633.00 2510535.00 5998474.00 6716032.00 10022992.00 12823796.00 8186459.00 C 1588301.00 1404414.00 7922100.00 7464766.00 9875595.00 9153699.00 10121324.00 9845688.00 12263271.00 3403162.00 D 4374207.00 3474890.00 9896490.00 13626569.00 4346024.00 10229690.00 13133277.00 17569282.00 483609.00 7213392.00 E 1753026.00 10752250.00 6961908.00 10499676.00 3809893.00 3197501.00 11345620.00 8522707.00 19372708.00 16586536.00 F 1283101.00 2625419.00 11728305.00 1476066.00 14211771.00 14226440.00 10421847.00 8714449.00 10433336.00 7529908.00 G 3535077.00 1379989.00 5159340.00 4413651.00 10247791.00 6240090.00 6418126.00 15929437.00 15730886.00 20109544.00 H 3705658.00 8197283.00 2938860.00 3749225.00 9202106.00 2596550.00 7843722.00 14890443.00 16797018.00 4805646.00 Load time: 05/21/2013 02:02:04 PM Read time: 05/21/2013 02:02:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3039 Comment: BioRep3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 16299139 cps Min counts: 190708 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3001952.00 6842287.00 3593799.00 4751431.00 7597696.00 12987711.00 3327589.00 4065060.00 7474741.00 7054668.00 B 2108829.00 6882531.00 3269884.00 7704235.00 4893418.00 15267800.00 8051939.00 8993563.00 2344392.00 4053840.00 C 4561581.00 6013989.00 5509064.00 6874856.00 6766055.00 14738129.00 6502605.00 6373355.00 7684395.00 3425580.00 D 1903749.00 695506.00 8048435.00 11469786.00 7115294.00 13380157.00 6644935.00 4122331.00 3519109.00 6056426.00 E 799963.00 8805150.00 7334349.00 12951009.00 12162166.00 15491708.00 10342270.00 9841253.00 9366292.00 11701253.00 F 4639870.00 8270299.00 7191506.00 11922697.00 8291088.00 13855013.00 381417.00 10277379.00 6131241.00 11860110.00 G 3172448.00 8560841.00 5357052.00 13233695.00 4425997.00 16299139.00 13060221.00 7303427.00 13095349.00 6463789.00 H 2983239.00 7558935.00 5817898.00 9050922.00 7893275.00 15649371.00 4644814.00 11326007.00 7823197.00 9822448.00 Load time: 05/21/2013 02:04:13 PM Read time: 05/21/2013 02:04:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3040 Comment: BioRep4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 26609588 cps Min counts: 229375 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10376262.00 7034795.00 18234642.00 10462704.00 15032978.00 3961085.00 13866593.00 4720454.00 9070055.00 9173325.00 B 11527949.00 7201802.00 8381246.00 26609588.00 6761038.00 9670919.00 20639122.00 4608545.00 7686304.00 10518747.00 C 14738493.00 5213446.00 8975011.00 8365875.00 18142304.00 6235072.00 17065674.00 4055039.00 6959189.00 13066954.00 D 10894718.00 757663.00 13496452.00 14742259.00 6833990.00 7766816.00 12539539.00 6086406.00 11349684.00 11731953.00 E 8150085.00 4807912.00 3840979.00 9905659.00 15979117.00 5242949.00 7231021.00 4893604.00 13985109.00 9707785.00 F 7100814.00 2642463.00 9189931.00 6394752.00 5214793.00 5169078.00 6639868.00 7841168.00 9134827.00 13136134.00 G 5828204.00 3605648.00 5275706.00 1552612.00 12799170.00 8971365.00 13480733.00 6411680.00 9432640.00 10751860.00 H 6006252.00 923881.00 458750.00 2412244.00 8770810.00 1948257.00 12682748.00 11991301.00 10628763.00 8577481.00 Load time: 05/21/2013 02:06:05 PM Read time: 05/21/2013 02:06:05 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3041 Comment: BioRep5 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 14141298 cps Min counts: 146747 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7716638.00 4487777.00 6882041.00 8986642.00 5452007.00 10944766.00 11226231.00 5410031.00 6104707.00 293495.00 B 6070221.00 1694630.00 6389663.00 9425831.00 6404351.00 10424742.00 8868164.00 4462894.00 12539092.00 10108752.00 C 9929002.00 4670173.00 8045237.00 6538101.00 9359058.00 9518213.00 14141298.00 7048243.00 10086134.00 11366670.00 D 7232536.00 2212036.00 7266225.00 6540634.00 10956632.00 11521422.00 12345891.00 9380203.00 11037075.00 11441034.00 E 9853155.00 6819866.00 9396207.00 9366157.00 12811451.00 12633981.00 11789504.00 4856268.00 11266311.00 11991115.00 F 9947499.00 7715776.00 9503915.00 7449856.00 10455546.00 11847406.00 8250091.00 7787571.00 12825158.00 8587638.00 G 6410675.00 8295164.00 7747476.00 6722005.00 10639983.00 13138768.00 11242164.00 7310423.00 11675948.00 11976720.00 H 7833121.00 9345066.00 7759365.00 9385736.00 8198118.00 10989133.00 11382434.00 7284882.00 10709104.00 7979489.00