Load time: 08/08/2013 12:15:37 PM Read time: 08/08/2013 12:15:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3173 Comment: Standards,T22 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 74732584 cps Min counts: 1228 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2627.00 66159076.00 65595260.00 74732584.00 274811.00 3600433.00 1859100.00 2523689.00 1814494.00 2756125.00 638246.00 5346832.00 B 2457.00 51779376.00 53937724.00 52371232.00 336363.00 1124407.00 1557578.00 3827567.00 1056881.00 3320412.00 1496028.00 6379187.00 C 2665.00 32665492.00 31627716.00 30596914.00 310559.00 4600728.00 1780672.00 4827210.00 1163634.00 4126471.00 2829932.00 8199407.00 D 2457.00 18370868.00 18336152.00 17713624.00 329388.00 4706524.00 299878.00 4044295.00 2676666.00 2875787.00 3894552.00 8359910.00 E 2589.00 11364252.00 10543548.00 10742691.00 351203.00 3439625.00 299424.00 6309435.00 296097.00 4464790.00 4558549.00 3804907.00 F 2627.00 6365150.00 6084904.00 6129963.00 339653.00 3291945.00 324227.00 5766435.00 293678.00 4143611.00 297080.00 5168313.00 G 2608.00 4091649.00 4028185.00 3814842.00 341808.00 3880665.00 290577.00 4512079.00 307931.00 4109728.00 252788.00 3072705.00 H 2722.00 2317008.00 2391275.00 2386626.00 375042.00 1905543.00 338065.00 2214711.00 302090.00 3402195.00 301107.00 3194419.00 Load time: 08/08/2013 12:18:27 PM Read time: 08/08/2013 12:18:27 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3174 Comment: Standard, Mily1-7 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 13652379 cps Min counts: 125458 counts Well list: r0(a1:h5) = cps(f2(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 741123.00 5119462.00 11422945.00 11072475.00 9285995.00 B 335966.00 7089602.00 7861117.00 11941851.00 10082124.00 C 254772.00 7335465.00 10531933.00 10463194.00 13504091.00 D 271011.00 254319.00 10794915.00 9693494.00 13652379.00 E 250916.00 279556.00 8769961.00 10926079.00 12164578.00 F 282335.00 273280.00 11519093.00 11107195.00 9945224.00 G 269783.00 293867.00 10246126.00 9157472.00 276834.00 H 265453.00 366440.00 10014410.00 13404992.00 280388.00