Load time: 09/20/2012 01:58:23 PM Read time: 09/20/2012 01:58:23 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2895 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 57183348 cps Min counts: 7462 counts Well list: r0(a2:h9) = cps(f2(a2:h9)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 51058364.00 53737400.00 57183348.00 23143.00 19988.00 15553446.00 12886174.00 16231761.00 B 49508072.00 53317912.00 45192064.00 22633.00 23427.00 20554570.00 12102311.00 19897274.00 C 34296272.00 54328196.00 23427.00 20460.00 12110701.00 24371.00 11683677.00 11685899.00 D 20673508.00 18579450.00 19541192.00 22954.00 8455944.00 21103.00 12698799.00 9404072.00 E 12481344.00 11850066.00 11979170.00 24390.00 8179800.00 22954.00 14925.00 6574616.00 F 7888547.00 8057093.00 7225437.00 20253.00 13586006.00 20064.00 18873.00 13764567.00 G 3833441.00 4256830.00 4031094.00 22293.00 11326060.00 23729.00 128679.00 10808439.00 H 2610427.00 2698453.00 2588216.00 24617.00 22425.00 22444.00 187398.00 10169268.00 Load time: 09/20/2012 02:00:29 PM Read time: 09/20/2012 02:00:29 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2896 Comment: test 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 60371256 cps Min counts: 1076 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2871.00 52007852.00 56518876.00 60371256.00 23105.00 19421.00 15516740.00 13294304.00 16759958.00 2380.00 2286.00 2380.00 B 2456.00 53325992.00 53869824.00 46809832.00 22331.00 22539.00 20693798.00 11831099.00 20215644.00 2342.00 2361.00 2361.00 C 2304.00 35859460.00 57431612.00 25410.00 21065.00 11865965.00 20782.00 13411718.00 12400997.00 2286.00 2153.00 2267.00 D 2399.00 21427348.00 19840550.00 20323420.00 23861.00 8929044.00 22180.00 12582183.00 9450217.00 2361.00 2361.00 2210.00 E 2418.00 13187102.00 12419228.00 12630195.00 24749.00 8191489.00 21726.00 14736.00 6291214.00 2248.00 2267.00 2437.00 F 2342.00 8254223.00 8567793.00 7545023.00 20744.00 14117553.00 21065.00 18552.00 13986552.00 2399.00 2342.00 2418.00 G 2342.00 3979682.00 4485959.00 4177679.00 23257.00 11740203.00 22784.00 129264.00 10904899.00 2267.00 2304.00 2323.00 H 2399.00 2724320.00 2769318.00 2727346.00 25146.00 22047.00 22746.00 187058.00 10665492.00 2399.00 2342.00 2418.00 Load time: 09/20/2012 02:06:17 PM Read time: 09/20/2012 02:06:17 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2897 Comment: after mix Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 55055228 cps Min counts: 1105 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3173.00 50023000.00 52475848.00 55055228.00 26128.00 54940.00 13013681.00 9859115.00 13634047.00 2267.00 2323.00 2267.00 B 2456.00 44934108.00 44679092.00 41156588.00 24352.00 18685.00 17066486.00 11442994.00 16392539.00 2361.00 2229.00 2418.00 C 2286.00 28525802.00 49870840.00 32061.00 23294.00 13393358.00 19270.00 11725012.00 12120299.00 2304.00 2210.00 2399.00 D 2474.00 16886424.00 16664602.00 16400877.00 75703.00 7465459.00 26563.00 9814650.00 7599751.00 2380.00 2248.00 2304.00 E 2342.00 10477592.00 9981849.00 10381727.00 26053.00 8224196.00 20101.00 17702.00 7438841.00 2418.00 2323.00 2286.00 F 2399.00 6625951.00 6431317.00 6016322.00 19761.00 11723094.00 20007.00 18401.00 11661053.00 2248.00 2323.00 2493.00 G 2380.00 3335014.00 3672360.00 3502081.00 21556.00 11095224.00 22085.00 76270.00 11377683.00 2380.00 2286.00 2474.00 H 2380.00 2195455.00 2072936.00 2181626.00 44417.00 53769.00 20706.00 136028.00 9751623.00 2531.00 2229.00 2493.00