##BLOCKS= 5 Plate: Plate3 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 25 -1906.375 32950741.625 31221229.625 25220797.625 -1640.375 16277411.625 8865159.625 10190159.625 1892820.625 865977.625 12295704.625 822757.625 -1998.375 30516233.625 26255007.625 23221947.625 -1580.375 4280822.625 10836912.625 8367648.625 17384.625 11838445.625 7207931.625 5610.625 -1869.375 28062579.625 26367097.625 20237441.625 -789.375 5716652.625 1057557.625 681807.625 3600062.625 11329620.625 8972357.625 5566527.625 -1190.375 19182831.625 18383261.625 17653779.625 -141.375 8339363.625 5314259.625 10929618.625 5797488.625 10638066.625 8169916.625 1193365.625 -2384.375 11023557.625 10569216.625 10445960.625 775.625 1599685.625 2460948.625 1847662.625 1683235.625 5845674.625 9189130.625 2584002.625 -1094.375 7323910.625 6644667.625 7471873.625 -611.375 5887166.625 11505086.625 7237633.625 10442896.625 6354953.625 11756059.625 697023.625 -1193.375 3832152.625 3486446.625 3676006.625 -1617.375 2044905.625 3650386.625 35956.625 958381.625 5633883.625 3502645.625 920983.625 -1600.375 2237150.625 2013762.625 2117497.625 5604.625 7831691.625 12089095.625 10477399.625 8162744.625 6295419.625 10093925.625 1575925.625 1 2 3 4 5 6 7 8 9 10 11 12 -1906.375 32950741.625 31221229.625 25220797.625 -1640.375 16277411.625 8865159.625 10190159.625 1892820.625 865977.625 12295704.625 822757.625 -1998.375 30516233.625 26255007.625 23221947.625 -1580.375 4280822.625 10836912.625 8367648.625 17384.625 11838445.625 7207931.625 5610.625 -1869.375 28062579.625 26367097.625 20237441.625 -789.375 5716652.625 1057557.625 681807.625 3600062.625 11329620.625 8972357.625 5566527.625 -1190.375 19182831.625 18383261.625 17653779.625 -141.375 8339363.625 5314259.625 10929618.625 5797488.625 10638066.625 8169916.625 1193365.625 -2384.375 11023557.625 10569216.625 10445960.625 775.625 1599685.625 2460948.625 1847662.625 1683235.625 5845674.625 9189130.625 2584002.625 -1094.375 7323910.625 6644667.625 7471873.625 -611.375 5887166.625 11505086.625 7237633.625 10442896.625 6354953.625 11756059.625 697023.625 -1193.375 3832152.625 3486446.625 3676006.625 -1617.375 2044905.625 3650386.625 35956.625 958381.625 5633883.625 3502645.625 920983.625 -1600.375 2237150.625 2013762.625 2117497.625 5604.625 7831691.625 12089095.625 10477399.625 8162744.625 6295419.625 10093925.625 1575925.625 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 24.5 -2151.5 29979768.5 28337832.5 23094280.5 -1547.5 14979366.5 8161403.5 9410490.5 1918677.5 884020.5 11382840.5 838560.5 -1626.5 28284782.5 24304104.5 21302556.5 -1792.5 4373956.5 10097399.5 7810079.5 15161.5 11100340.5 6709005.5 5320.5 -1505.5 26015366.5 24175162.5 20238846.5 -736.5 5782683.5 1076881.5 730499.5 3662470.5 11546108.5 9180831.5 5654358.5 -602.5 19486584.5 18522902.5 17766382.5 67.5 8469537.5 5439857.5 11043516.5 5939595.5 10912046.5 8342277.5 1202678.5 -1988.5 11177148.5 10774582.5 10581605.5 841.5 1640172.5 2604711.5 1889739.5 1727635.5 5494420.5 8656611.5 2650877.5 -600.5 6831512.5 6197650.5 6930194.5 -740.5 5524957.5 10833613.5 6785590.5 9811787.5 6014100.5 11106263.5 726371.5 -984.5 3900747.5 3565017.5 3771087.5 -1391.5 2102393.5 3745999.5 37884.5 990329.5 5289581.5 3676717.5 960016.5 -1569.5 2295926.5 2100381.5 2175632.5 5299.5 8192713.5 12616866.5 10989663.5 8587643.5 6619079.5 10697173.5 1638859.5 1 2 3 4 5 6 7 8 9 10 11 12 -2151.5 29979768.5 28337832.5 23094280.5 -1547.5 14979366.5 8161403.5 9410490.5 1918677.5 884020.5 11382840.5 838560.5 -1626.5 28284782.5 24304104.5 21302556.5 -1792.5 4373956.5 10097399.5 7810079.5 15161.5 11100340.5 6709005.5 5320.5 -1505.5 26015366.5 24175162.5 20238846.5 -736.5 5782683.5 1076881.5 730499.5 3662470.5 11546108.5 9180831.5 5654358.5 -602.5 19486584.5 18522902.5 17766382.5 67.5 8469537.5 5439857.5 11043516.5 5939595.5 10912046.5 8342277.5 1202678.5 -1988.5 11177148.5 10774582.5 10581605.5 841.5 1640172.5 2604711.5 1889739.5 1727635.5 5494420.5 8656611.5 2650877.5 -600.5 6831512.5 6197650.5 6930194.5 -740.5 5524957.5 10833613.5 6785590.5 9811787.5 6014100.5 11106263.5 726371.5 -984.5 3900747.5 3565017.5 3771087.5 -1391.5 2102393.5 3745999.5 37884.5 990329.5 5289581.5 3676717.5 960016.5 -1569.5 2295926.5 2100381.5 2175632.5 5299.5 8192713.5 12616866.5 10989663.5 8587643.5 6619079.5 10697173.5 1638859.5 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 1 100.000 78.389 A2 29979768.500 30581929.486 5409311.286 17.7 72.880 A3 28337832.500 55.285 A4 23094280.500 119.739 A2 42302481.000 82.381 A3 31169409.000 81.681 A4 30960825.000 88.359 A2 32950741.625 82.555 A3 31221229.625 62.420 A4 25220797.625 2 60.000 72.702 B2 28284782.500 27925925.486 5083179.001 18.2 59.344 B3 24304104.500 49.272 B4 21302556.500 107.215 B2 38570077.000 77.274 B3 29647385.000 75.878 B4 29231235.000 80.190 B2 30516233.625 65.891 B3 26255007.625 55.713 B4 23221947.625 3 36.000 65.087 C2 26015366.500 25922065.931 4152006.329 16.0 58.912 C3 24175162.500 45.703 C4 20238846.500 90.158 C2 33486987.000 74.401 C3 28791047.000 64.780 C4 25924065.000 71.956 C2 28062579.625 66.267 C3 26367097.625 45.698 C4 20237441.625 4 21.600 43.179 D2 19486584.500 18514395.264 740808.945 4.0 39.945 D3 18522902.500 37.406 D4 17766382.500 42.726 D2 19351749.000 40.650 D3 18733069.000 36.677 D4 17548997.000 42.159 D2 19182831.625 39.476 D3 18383261.625 37.029 D4 17653779.625 5 12.960 15.296 E2 11177148.500 10818669.597 282396.317 2.6 13.945 E3 10774582.500 13.297 E4 10581605.500 14.957 E2 11076189.000 15.114 E3 11123037.000 13.348 E4 10596729.000 14.780 E2 11023557.625 13.256 E3 10569216.625 12.842 E4 10445960.625 6 7.770 0.714 F2 6831512.500 6702568.486 474157.895 7.1 -1.413 F3 6197650.500 1.045 F4 6930194.500 -1.468 F2 6181478.000 -0.715 F3 6405917.000 -0.949 F4 6335912.000 2.366 F2 7323910.625 0.087 F3 6644667.625 2.862 F4 7471873.625 7 4.670 -9.121 G2 3900747.500 3639590.708 179216.319 4.9 -10.247 G3 3565017.500 -9.556 G4 3771087.500 -9.816 G2 3693568.000 -10.681 G3 3435847.000 -10.816 G4 3395443.000 -9.351 G2 3832152.625 -10.511 G3 3486446.625 -9.875 G4 3676006.625 8 2.800 -14.506 H2 2295926.500 2096878.819 147086.123 7.0 -15.162 H3 2100381.500 -14.910 H4 2175632.500 -14.924 H2 2171490.000 -15.961 H3 1862421.000 -15.842 H4 1897647.000 -14.703 H2 2237150.625 -15.453 H3 2013762.625 -15.105 H4 2117497.625 Group Summaries MinStd Smallest standard value: 2096878.819 Min(MeanValue) MaxStd Largest standard value: 30581929.486 Max(MeanValue) ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 25 102 42302481 31169409 30960825 -563 609618 2755345 4152577 8891696 3838011 -646 -6454 1396 38570077 29647385 29231235 -620 3317 1921668 2081 -683 18361773 -868 -6318 -608 33486987 28791047 25924065 -668 1188524 3304175 1496963 3499019 2834335 -1366 -6406 1733 19351749 18733069 17548997 -949 1497 3560348 2184566 7553918 3878370 1596 -6409 -48 11076189 11123037 10596729 -125 1727745 1680006 1043259 3786663 1210183 1105 -6504 -1025 6181478 6405917 6335912 -286 2080831 4357133 3283388 5294088 7215189 -1286 -6496 1506 3693568 3435847 3395443 2003 1361629 3747 2025627 1840345 1045286 -1370 -6493 53 2171490 1862421 1897647 1208 2468319 1419245 1187736 8967812 3464630 -1035 -6322 1 2 3 4 5 6 7 8 9 10 11 12 102 42302481 31169409 30960825 -563 609618 2755345 4152577 8891696 3838011 -646 -6454 1396 38570077 29647385 29231235 -620 3317 1921668 2081 -683 18361773 -868 -6318 -608 33486987 28791047 25924065 -668 1188524 3304175 1496963 3499019 2834335 -1366 -6406 1733 19351749 18733069 17548997 -949 1497 3560348 2184566 7553918 3878370 1596 -6409 -48 11076189 11123037 10596729 -125 1727745 1680006 1043259 3786663 1210183 1105 -6504 -1025 6181478 6405917 6335912 -286 2080831 4357133 3283388 5294088 7215189 -1286 -6496 1506 3693568 3435847 3395443 2003 1361629 3747 2025627 1840345 1045286 -1370 -6493 53 2171490 1862421 1897647 1208 2468319 1419245 1187736 8967812 3464630 -1035 -6322 ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A6 14979366.500 28.054 -6.488 14.075 217.0 A7 8161403.500 5.176 A8 9410490.500 9.368 A9 1918677.500 R -15.772 A10 884020.500 R -19.244 A11 11382840.500 15.986 A12 838560.500 R -19.396 B6 4373956.500 -7.533 B7 10097399.500 11.673 B8 7810079.500 3.997 B9 15161.500 R -22.159 B10 11100340.500 15.038 B11 6709005.500 0.302 B12 5320.500 R -22.192 C6 5782683.500 -2.806 C7 1076881.500 R -18.597 C8 730499.500 R -19.759 C9 3662470.500 -9.920 C10 11546108.500 16.534 C11 9180831.500 8.597 C12 5654358.500 -3.236 D6 8469537.500 6.210 D7 5439857.500 -3.956 D8 11043516.500 14.847 D9 5939595.500 -2.279 D10 10912046.500 14.406 D11 8342277.500 5.783 D12 1202678.500 R -18.174 E6 1640172.500 R -16.706 E7 2604711.500 -13.470 E8 1889739.500 R -15.869 E9 1727635.500 R -16.413 E10 5494420.500 -3.773 E11 8656611.500 6.838 E12 2650877.500 -13.315 F6 5524957.500 -3.671 F7 10833613.500 14.143 F8 6785590.500 0.559 F9 9811787.500 10.714 F10 6014100.500 -2.029 F11 11106263.500 15.058 F12 726371.500 R -19.773 G6 2102393.500 -15.155 G7 3745999.500 -9.640 G8 37884.500 R -22.083 G9 990329.500 R -18.887 G10 5289581.500 -4.461 G11 3676717.500 -9.873 G12 960016.500 R -18.989 H6 8192713.500 5.281 H7 12616866.500 20.127 H8 10989663.500 14.667 H9 8587643.500 6.606 H10 6619079.500 0.001 H11 10697173.500 13.685 H12 1638859.500 R -16.711 A6 609618.000 R -20.165 A7 2755345.000 -12.964 A8 4152577.000 -8.276 A9 8891696.000 7.627 A10 3838011.000 -9.331 A11 -646.000 R -22.212 A12 -6454.000 R -22.232 B6 3317.000 R -22.199 B7 1921668.000 R -15.762 B8 2081.000 R -22.203 B9 -683.000 R -22.212 B10 18361773.000 39.404 B11 -868.000 R -22.213 B12 -6318.000 R -22.231 C6 1188524.000 R -18.222 C7 3304175.000 -11.123 C8 1496963.000 R -17.187 C9 3499019.000 -10.469 C10 2834335.000 -12.699 C11 -1366.000 R -22.215 C12 -6406.000 R -22.232 D6 1497.000 R -22.205 D7 3560348.000 -10.263 D8 2184566.000 -14.880 D9 7553918.000 3.138 D10 3878370.000 -9.196 D11 1596.000 R -22.205 D12 -6409.000 R -22.232 E6 1727745.000 R -16.413 E7 1680006.000 R -16.573 E8 1043259.000 R -18.709 E9 3786663.000 -9.504 E10 1210183.000 R -18.149 E11 1105.000 R -22.206 E12 -6504.000 R -22.232 F6 2080831.000 R -15.228 F7 4357133.000 -7.589 F8 3283388.000 -11.192 F9 5294088.000 -4.445 F10 7215189.000 2.001 F11 -1286.000 R -22.214 F12 -6496.000 R -22.232 G6 1361629.000 R -17.641 G7 3747.000 R -22.198 G8 2025627.000 R -15.413 G9 1840345.000 R -16.035 G10 1045286.000 R -18.703 G11 -1370.000 R -22.215 G12 -6493.000 R -22.232 H6 2468319.000 -13.927 H7 1419245.000 R -17.448 H8 1187736.000 R -18.225 H9 8967812.000 7.882 H10 3464630.000 -10.584 H11 -1035.000 R -22.214 H12 -6322.000 R -22.231 A6 16277411.625 32.410 A7 8865159.625 7.538 A8 10190159.625 11.984 A9 1892820.625 R -15.859 A10 865977.625 R -19.304 A11 12295704.625 19.049 A12 822757.625 R -19.449 B6 4280822.625 -7.845 B7 10836912.625 14.154 B8 8367648.625 5.868 B9 17384.625 R -22.152 B10 11838445.625 17.515 B11 7207931.625 1.977 B12 5610.625 R -22.191 C6 5716652.625 -3.027 C7 1057557.625 R -18.661 C8 681807.625 R -19.922 C9 3600062.625 -10.130 C10 11329620.625 15.807 C11 8972357.625 7.897 C12 5566527.625 -3.531 D6 8339363.625 5.773 D7 5314259.625 -4.378 D8 10929618.625 14.465 D9 5797488.625 -2.756 D10 10638066.625 13.487 D11 8169916.625 5.205 D12 1193365.625 R -18.206 E6 1599685.625 R -16.842 E7 2460948.625 -13.952 E8 1847662.625 R -16.010 E9 1683235.625 R -16.562 E10 5845674.625 -2.595 E11 9189130.625 8.625 E12 2584002.625 -13.539 F6 5887166.625 -2.455 F7 11505086.625 16.396 F8 7237633.625 2.076 F9 10442896.625 12.832 F10 6354953.625 -0.886 F11 11756059.625 17.238 F12 697023.625 R -19.871 G6 2044905.625 R -15.348 G7 3650386.625 -9.961 G8 35956.625 R -22.089 G9 958381.625 R -18.994 G10 5633883.625 -3.305 G11 3502645.625 -10.457 G12 920983.625 R -19.120 H6 7831691.625 4.070 H7 12089095.625 18.356 H8 10477399.625 12.948 H9 8162744.625 5.181 H10 6295419.625 -1.085 H11 10093925.625 11.661 H12 1575925.625 R -16.922 Group Summaries InRange R - Outside standard range ~End Original Filename: 140814_plates1thru3; Date Last Saved: 8/14/2014 5:03:40 PM