##BLOCKS= 4 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 26.5 1489 27242498 30008276 29036448 8891 1279404 10228 39778 8539 8508 10092 8555 1337 27382186 31980264 28622560 8553 2488965 8572 8311 8734 9715 9279 10681 1261 26971848 30150302 28176776 8971 8443 8978 8328 8426 9772 8525 8093 1440 18772290 16985008 21012534 8598 8453 7960 8328 14924 10853 14419 8488 1430 11739953 9855197 12162845 13527 8632 9095 8766 29392 6975 7227 8767 1710 7277166 6432171 7781705 12035 9174 8239 8918 8463 7955 10398 7662 1327 4254047 3562468 4109105 9585 8151 11112 12739 13041 8253 8406 7729 1457 3144638 2484785 2451925 9302 17373 17603 16780 7499 9339 14071 8229 1 2 3 4 5 6 7 8 9 10 11 12 1489 27242498 30008276 29036448 8891 1279404 10228 39778 8539 8508 10092 8555 1337 27382186 31980264 28622560 8553 2488965 8572 8311 8734 9715 9279 10681 1261 26971848 30150302 28176776 8971 8443 8978 8328 8426 9772 8525 8093 1440 18772290 16985008 21012534 8598 8453 7960 8328 14924 10853 14419 8488 1430 11739953 9855197 12162845 13527 8632 9095 8766 29392 6975 7227 8767 1710 7277166 6432171 7781705 12035 9174 8239 8918 8463 7955 10398 7662 1327 4254047 3562468 4109105 9585 8151 11112 12739 13041 8253 8406 7729 1457 3144638 2484785 2451925 9302 17373 17603 16780 7499 9339 14071 8229 ~End Group: Group01 Sample Well Concentration Values MeanValue Std.Dev. CV% 01 A2 100.000 27242498.000 28762407.333 1403105.734 4.878 A3 30008276.000 A4 29036448.000 02 B2 60.000 27382186.000 29328336.667 2378901.186 8.111 B3 31980264.000 B4 28622560.000 03 C2 36.000 26971848.000 28432975.333 1604640.468 5.644 C3 30150302.000 C4 28176776.000 04 D2 21.600 18772290.000 18923277.333 2018003.791 10.664 D3 16985008.000 D4 21012534.000 05 E2 12.960 11739953.000 11252665.000 1228575.199 10.918 E3 9855197.000 E4 12162845.000 06 F2 7.770 7277166.000 7163680.667 681886.864 9.519 F3 6432171.000 F4 7781705.000 07 G2 4.670 4254047.000 3975206.667 364714.890 9.175 G3 3562468.000 G4 4109105.000 08 H2 2.800 3144638.000 2693782.667 390797.702 14.507 H3 2484785.000 H4 2451925.000 Group Summaries ~End Group: Group02 Sample Well Values Result MeanResult Std.Dev. CV% 01 A6 1279404.000 B6 2488965.000 Group Summaries ~End Original Filename: 140918_lib_redos; Date Last Saved: 9/18/2014 11:08:00 AM