Load time: 07/07/2011 01:53:35 PM Read time: 07/07/2011 01:53:35 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1659 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 84287112 cps Min counts: 62886 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 84287112.00 56590864.00 60497772.00 660582.00 2478398.00 1306640.00 5003334.00 5778434.00 1714890.00 125773.00 B 39106048.00 44105456.00 35678632.00 802270.00 2240662.00 1560365.00 4151348.00 1544786.00 4789387.00 128419.00 C 21928936.00 20338186.00 19792250.00 1340715.00 1516041.00 1937985.00 2762424.00 2393720.00 5605695.00 134873.00 D 12498602.00 13142370.00 11452292.00 1040435.00 1178106.00 1556931.00 3670373.00 2300999.00 5369848.00 131351.00 E 7273655.00 6521626.00 6053840.00 813140.00 1102265.00 465059.00 3671960.00 3434978.00 2586347.00 132226.00 F 3696743.00 3486551.00 3724704.00 1017572.00 1271861.00 1924972.00 9661526.00 2259331.00 2404564.00 130856.00 G 1863604.00 2031573.00 1923093.00 1158822.00 1300777.00 2432657.00 3858162.00 5270593.00 2428602.00 139384.00 H 967411.00 1002210.00 1006569.00 864576.00 916641.00 1214828.00 1639513.00 2936460.00 1795781.00 129618.00