Load time: 07/07/2011 01:58:07 PM Read time: 07/07/2011 01:58:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1660 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 84333600 cps Min counts: 59355 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 84333600.00 54730296.00 58836116.00 636406.00 2402242.00 1253567.00 4841871.00 5498804.00 1654461.00 118711.00 B 37204480.00 42850400.00 34087816.00 778056.00 2174865.00 1514080.00 4069065.00 1492474.00 4763130.00 121223.00 C 20848326.00 19495334.00 18950408.00 1281150.00 1450613.00 1858706.00 2646411.00 2300676.00 5524533.00 127068.00 D 12171704.00 12818081.00 10798619.00 991492.00 1126061.00 1485297.00 3550058.00 2234568.00 5221194.00 122842.00 E 7177826.00 6165655.00 5733320.00 776057.00 1054027.00 446099.00 3536810.00 3251359.00 2447640.00 155908.00 F 3514120.00 3323856.00 3690411.00 976891.00 1228039.00 1834961.00 9494135.00 2186394.00 2359742.00 122956.00 G 1767899.00 1969145.00 1850014.00 1101352.00 1242278.00 2343829.00 3818488.00 5100598.00 2286448.00 125373.00 H 932194.00 966707.00 938076.00 825057.00 874513.00 1200189.00 1590594.00 2851488.00 1724480.00 119072.00