Load time: 05/12/2011 12:16:26 PM Read time: 05/12/2011 12:16:26 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1544 Comment: 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 149409600 cps Min counts: 59229 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 149409600.00 113576824.00 109378776.00 118459.00 5097479.00 17410790.00 3578681.00 16021983.00 16000867.00 30450316.00 32170600.00 19693302.00 B 62670764.00 65512536.00 62810144.00 120724.00 12844511.00 48736348.00 6466242.00 36985344.00 23620994.00 1369051.00 33239306.00 4977852.00 C 39378152.00 40248012.00 37928764.00 125405.00 26812946.00 33243272.00 27761534.00 44244228.00 12621670.00 11536203.00 1050076.00 28842852.00 D 23148670.00 24064830.00 23033474.00 123293.00 15951486.00 2340443.00 5541593.00 7520300.00 14120512.00 16038236.00 4809476.00 9544624.00 E 14805167.00 14390488.00 13852078.00 124663.00 13240457.00 18086984.00 9021105.00 24706720.00 5040283.00 4022057.00 16190985.00 2405008.00 F 8081835.00 7921629.00 8359322.00 122855.00 16686112.00 15356904.00 17942596.00 26340684.00 20633822.00 7363276.00 8883974.00 12899588.00 G 5451161.00 5839327.00 5511329.00 124149.00 2176168.00 6566718.00 9529349.00 11594530.00 22428424.00 32598178.00 38635080.00 547673.00 H 2630061.00 2724271.00 2883841.00 120781.00 3443992.00 2285407.00 12114570.00 9759999.00 4889382.00 16860300.00 4867317.00 22372996.00 Load time: 05/12/2011 12:18:38 PM Read time: 05/12/2011 12:18:38 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1545 Comment: 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 82143880 cps Min counts: 51950 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 82143880.00 74430168.00 78647024.00 103901.00 6160998.00 10488939.00 341030.00 3049041.00 876677.00 10336654.00 19608634.00 125595.00 B 58741232.00 41917000.00 50540776.00 110505.00 17691688.00 18362886.00 32652116.00 32993504.00 10756845.00 6807622.00 23984486.00 132084.00 C 37114256.00 31139588.00 36536156.00 120248.00 31658014.00 24324448.00 21354172.00 18801982.00 7358514.00 3162539.00 27976162.00 132065.00 D 22764658.00 22176938.00 22100852.00 122988.00 3940974.00 5649048.00 22412882.00 13544621.00 14902797.00 5055681.00 15400292.00 132884.00 E 14385096.00 13874368.00 13454446.00 118916.00 11091546.00 13614270.00 6319841.00 6204340.00 4111522.00 11055363.00 44035512.00 27230918.00 F 7643064.00 8225622.00 7726832.00 118726.00 7333687.00 11487865.00 18858758.00 23836328.00 10341852.00 11771471.00 39774000.00 225577.00 G 4798554.00 4840536.00 4690248.00 121238.00 13277354.00 339489.00 999837.00 1884470.00 35730888.00 6873119.00 12835521.00 9926164.00 H 2961597.00 2910234.00 2814275.00 118783.00 354161.00 7438350.00 1284730.00 8678469.00 4621545.00 37876784.00 40384596.00 18901426.00