Load time: 12/09/2010 11:35:00 AM Read time: 12/09/2010 11:35:00 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1219 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 24661858 cps Min counts: 923 counts Well list: r0(a1:h6) = cps(f2(a1:h6)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15315935.00 24661858.00 17676768.00 4536434.00 1060619.00 1885.00 B 9790122.00 8832047.00 10399655.00 1338084.00 256918.00 2075.00 C 5928875.00 5530614.00 4619577.00 6363382.00 214283.00 1885.00 D 3592010.00 3765243.00 3967291.00 4369068.00 282130.00 1904.00 E 2040964.00 2140269.00 3802347.00 1504114.00 264097.00 1885.00 F 1857674.00 1125553.00 971653.00 3862859.00 282169.00 1904.00 G 674364.00 907613.00 1299200.00 9589849.00 290585.00 2189.00 H 560834.00 1068883.00 529814.00 7284560.00 258309.00 1847.00 Load time: 12/09/2010 11:35:00 AM Read time: 12/09/2010 11:35:00 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1219 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 24661858 cps Min counts: 923 counts Well list: r0(a1:h6) = cps(f2(a1:h6)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15315935.00 24661858.00 17676768.00 4536434.00 1060619.00 1885.00 B 9790122.00 8832047.00 10399655.00 1338084.00 256918.00 2075.00 C 5928875.00 5530614.00 4619577.00 6363382.00 214283.00 1885.00 D 3592010.00 3765243.00 3967291.00 4369068.00 282130.00 1904.00 E 2040964.00 2140269.00 3802347.00 1504114.00 264097.00 1885.00 F 1857674.00 1125553.00 971653.00 3862859.00 282169.00 1904.00 G 674364.00 907613.00 1299200.00 9589849.00 290585.00 2189.00 H 560834.00 1068883.00 529814.00 7284560.00 258309.00 1847.00 Load time: 12/09/2010 11:35:00 AM Read time: 12/09/2010 11:35:00 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1219 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 24661858 cps Min counts: 923 counts Well list: r0(a1:h6) = cps(f2(a1:h6)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15315935.00 24661858.00 17676768.00 4536434.00 1060619.00 1885.00 B 9790122.00 8832047.00 10399655.00 1338084.00 256918.00 2075.00 C 5928875.00 5530614.00 4619577.00 6363382.00 214283.00 1885.00 D 3592010.00 3765243.00 3967291.00 4369068.00 282130.00 1904.00 E 2040964.00 2140269.00 3802347.00 1504114.00 264097.00 1885.00 F 1857674.00 1125553.00 971653.00 3862859.00 282169.00 1904.00 G 674364.00 907613.00 1299200.00 9589849.00 290585.00 2189.00 H 560834.00 1068883.00 529814.00 7284560.00 258309.00 1847.00