Load time: 03/18/2011 12:18:08 PM Read time: 03/18/2011 12:18:08 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1394 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 94528136 cps Min counts: 54456 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 88983024.00 92271560.00 94528136.00 2130766.00 1472857.00 1166415.00 2285255.00 6612783.00 4184292.00 2281410.00 124894.00 114373.00 B 57198984.00 59426296.00 56535004.00 1306262.00 879928.00 875990.00 4056521.00 4312189.00 4193251.00 3418807.00 121698.00 114392.00 C 41714396.00 46546120.00 62944436.00 2004045.00 806951.00 1146458.00 2573960.00 1698660.00 2649537.00 3413179.00 119167.00 114830.00 D 19955882.00 19448304.00 21161306.00 2962727.00 1555666.00 1029459.00 2061495.00 4586488.00 1758558.00 4368660.00 121336.00 115229.00 E 15519545.00 18753344.00 17155112.00 1677155.00 1079074.00 1427294.00 3121034.00 2424780.00 3633788.00 122440.00 113156.00 112851.00 F 11116086.00 10486546.00 9919942.00 592091.00 903823.00 1250540.00 1453072.00 1940589.00 3470355.00 122649.00 120252.00 113308.00 G 6837065.00 4943686.00 4445301.00 3910818.00 3594705.00 1170238.00 1897936.00 2213078.00 5037871.00 123828.00 120024.00 109541.00 H 4237851.00 4103093.00 2596610.00 2561766.00 3236034.00 1806795.00 1157987.00 5462282.00 3056217.00 123220.00 116942.00 108913.00 Load time: 03/18/2011 12:18:08 PM Read time: 03/18/2011 12:18:08 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1394 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 94528136 cps Min counts: 54456 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 88983024.00 92271560.00 94528136.00 2130766.00 1472857.00 1166415.00 2285255.00 6612783.00 4184292.00 2281410.00 124894.00 114373.00 B 57198984.00 59426296.00 56535004.00 1306262.00 879928.00 875990.00 4056521.00 4312189.00 4193251.00 3418807.00 121698.00 114392.00 C 41714396.00 46546120.00 62944436.00 2004045.00 806951.00 1146458.00 2573960.00 1698660.00 2649537.00 3413179.00 119167.00 114830.00 D 19955882.00 19448304.00 21161306.00 2962727.00 1555666.00 1029459.00 2061495.00 4586488.00 1758558.00 4368660.00 121336.00 115229.00 E 15519545.00 18753344.00 17155112.00 1677155.00 1079074.00 1427294.00 3121034.00 2424780.00 3633788.00 122440.00 113156.00 112851.00 F 11116086.00 10486546.00 9919942.00 592091.00 903823.00 1250540.00 1453072.00 1940589.00 3470355.00 122649.00 120252.00 113308.00 G 6837065.00 4943686.00 4445301.00 3910818.00 3594705.00 1170238.00 1897936.00 2213078.00 5037871.00 123828.00 120024.00 109541.00 H 4237851.00 4103093.00 2596610.00 2561766.00 3236034.00 1806795.00 1157987.00 5462282.00 3056217.00 123220.00 116942.00 108913.00