Load time: 09/03/2010 10:06:43 AM Read time: 09/03/2010 10:06:43 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1022 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 30700164 cps Min counts: 88024 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2062137.00 3989564.00 4102503.00 7382453.00 8238364.00 4541963.00 1905700.00 2025545.00 2324369.00 3543255.00 1633607.00 6336640.00 B 3425434.00 4105158.00 10932570.00 2885599.00 2035227.00 802151.00 2776290.00 6435611.00 3211121.00 1937545.00 5389845.00 3110197.00 C 2072311.00 176048.00 12819101.00 8361799.00 5365100.00 4275720.00 8705087.00 605723.00 3878990.00 2672191.00 1575738.00 4411282.00 D 8877718.00 2249390.00 9535289.00 1962072.00 1162324.00 3362810.00 2829145.00 2258441.00 5188544.00 2170223.00 2637075.00 8237921.00 E 3551022.00 10575572.00 7873306.00 5209561.00 1665407.00 5128672.00 2565420.00 1896874.00 1653413.00 1818647.00 1499813.00 2373740.00 F 3694931.00 12323827.00 3618441.00 4142736.00 1280261.00 7985201.00 3906399.00 4910795.00 16424644.00 7122177.00 1847139.00 2431642.00 G 6061311.00 7190213.00 6076863.00 4543645.00 6285901.00 6423673.00 9981469.00 4039020.00 2309135.00 2772424.00 3947261.00 7787896.00 H 7035386.00 4003479.00 2034251.00 4348460.00 1820412.00 4944039.00 7128704.00 922907.00 267168.00 5968799.00 30700164.00 3499427.00 Load time: 09/03/2010 10:06:43 AM Read time: 09/03/2010 10:06:43 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1022 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 30700164 cps Min counts: 88024 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2062137.00 3989564.00 4102503.00 7382453.00 8238364.00 4541963.00 1905700.00 2025545.00 2324369.00 3543255.00 1633607.00 6336640.00 B 3425434.00 4105158.00 10932570.00 2885599.00 2035227.00 802151.00 2776290.00 6435611.00 3211121.00 1937545.00 5389845.00 3110197.00 C 2072311.00 176048.00 12819101.00 8361799.00 5365100.00 4275720.00 8705087.00 605723.00 3878990.00 2672191.00 1575738.00 4411282.00 D 8877718.00 2249390.00 9535289.00 1962072.00 1162324.00 3362810.00 2829145.00 2258441.00 5188544.00 2170223.00 2637075.00 8237921.00 E 3551022.00 10575572.00 7873306.00 5209561.00 1665407.00 5128672.00 2565420.00 1896874.00 1653413.00 1818647.00 1499813.00 2373740.00 F 3694931.00 12323827.00 3618441.00 4142736.00 1280261.00 7985201.00 3906399.00 4910795.00 16424644.00 7122177.00 1847139.00 2431642.00 G 6061311.00 7190213.00 6076863.00 4543645.00 6285901.00 6423673.00 9981469.00 4039020.00 2309135.00 2772424.00 3947261.00 7787896.00 H 7035386.00 4003479.00 2034251.00 4348460.00 1820412.00 4944039.00 7128704.00 922907.00 267168.00 5968799.00 30700164.00 3499427.00