Load time: 09/03/2010 10:09:57 AM Read time: 09/03/2010 10:09:57 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1023 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 56404860 cps Min counts: 105366 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9390287.00 6277544.00 3718266.00 3078373.00 2374958.00 12342175.00 8859068.00 2521494.00 4648521.00 5460140.00 5705176.00 2116728.00 B 3681282.00 3870723.00 9514867.00 2517496.00 931116.00 16361196.00 7411002.00 25000562.00 2764898.00 3813213.00 13034674.00 2426845.00 C 3431522.00 9033046.00 4273223.00 24549542.00 2683581.00 3653170.00 1126326.00 1092956.00 6081700.00 10936937.00 3755395.00 8053636.00 D 4221979.00 4599351.00 7121741.00 2187323.00 8895524.00 5480204.00 3862799.00 433426.00 1976476.00 4301878.00 14268725.00 2433514.00 E 2004927.00 7010017.00 9114016.00 3546497.00 17424894.00 1333916.00 2135398.00 14308557.00 17017142.00 8017295.00 2676978.00 9579569.00 F 6327122.00 2865172.00 2857369.00 11742000.00 4160980.00 7728506.00 5989268.00 1945839.00 18511152.00 11921392.00 8661366.00 3963676.00 G 12018989.00 210732.00 6093819.00 43947056.00 18727466.00 2079510.00 3450323.00 4432236.00 6838086.00 7702350.00 5413294.00 5415334.00 H 2944728.00 5053924.00 662977.00 7806522.00 5352947.00 2977170.00 5451624.00 56404860.00 2706031.00 10236811.00 2673717.00 6509130.00 Load time: 09/03/2010 10:09:57 AM Read time: 09/03/2010 10:09:57 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1023 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 56404860 cps Min counts: 105366 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9390287.00 6277544.00 3718266.00 3078373.00 2374958.00 12342175.00 8859068.00 2521494.00 4648521.00 5460140.00 5705176.00 2116728.00 B 3681282.00 3870723.00 9514867.00 2517496.00 931116.00 16361196.00 7411002.00 25000562.00 2764898.00 3813213.00 13034674.00 2426845.00 C 3431522.00 9033046.00 4273223.00 24549542.00 2683581.00 3653170.00 1126326.00 1092956.00 6081700.00 10936937.00 3755395.00 8053636.00 D 4221979.00 4599351.00 7121741.00 2187323.00 8895524.00 5480204.00 3862799.00 433426.00 1976476.00 4301878.00 14268725.00 2433514.00 E 2004927.00 7010017.00 9114016.00 3546497.00 17424894.00 1333916.00 2135398.00 14308557.00 17017142.00 8017295.00 2676978.00 9579569.00 F 6327122.00 2865172.00 2857369.00 11742000.00 4160980.00 7728506.00 5989268.00 1945839.00 18511152.00 11921392.00 8661366.00 3963676.00 G 12018989.00 210732.00 6093819.00 43947056.00 18727466.00 2079510.00 3450323.00 4432236.00 6838086.00 7702350.00 5413294.00 5415334.00 H 2944728.00 5053924.00 662977.00 7806522.00 5352947.00 2977170.00 5451624.00 56404860.00 2706031.00 10236811.00 2673717.00 6509130.00