Load time: 09/03/2010 10:12:42 AM Read time: 09/03/2010 10:12:42 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1024 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 54144552 cps Min counts: 103614 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6992331.00 4516870.00 6585149.00 34133980.00 4485770.00 2499874.00 213761.00 7361117.00 7675441.00 19911552.00 2043450.00 3401566.00 B 5541336.00 5997538.00 3477187.00 6341883.00 4800593.00 1567560.00 3148493.00 8440725.00 6186158.00 8512024.00 2062169.00 1092194.00 C 8475460.00 4774735.00 17106432.00 2456353.00 15224658.00 1422178.00 1618765.00 5291505.00 7274095.00 10038145.00 5660671.00 16328049.00 D 2039099.00 2855652.00 16497338.00 18447598.00 2069406.00 3485388.00 37490032.00 207685.00 5192940.00 28807686.00 2169467.00 4511550.00 E 34492096.00 3738479.00 16657431.00 15046452.00 2635114.00 9086090.00 9740121.00 207228.00 11455918.00 21468268.00 3424568.00 19992840.00 F 15911221.00 28039338.00 6636354.00 8852655.00 23304136.00 207894.00 9333822.00 9921629.00 2068762.00 1889641.00 10532312.00 6112591.00 G 42865528.00 54144552.00 9263753.00 3489705.00 8983121.00 26659758.00 9661069.00 2656529.00 16874038.00 9223930.00 2341123.00 822074.00 H 2918314.00 4115557.00 10199553.00 3209532.00 220065.00 11204903.00 4102788.00 12360948.00 295147.00 11379835.00 1821829.00 5143926.00 Load time: 09/03/2010 10:12:42 AM Read time: 09/03/2010 10:12:42 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1024 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 54144552 cps Min counts: 103614 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6992331.00 4516870.00 6585149.00 34133980.00 4485770.00 2499874.00 213761.00 7361117.00 7675441.00 19911552.00 2043450.00 3401566.00 B 5541336.00 5997538.00 3477187.00 6341883.00 4800593.00 1567560.00 3148493.00 8440725.00 6186158.00 8512024.00 2062169.00 1092194.00 C 8475460.00 4774735.00 17106432.00 2456353.00 15224658.00 1422178.00 1618765.00 5291505.00 7274095.00 10038145.00 5660671.00 16328049.00 D 2039099.00 2855652.00 16497338.00 18447598.00 2069406.00 3485388.00 37490032.00 207685.00 5192940.00 28807686.00 2169467.00 4511550.00 E 34492096.00 3738479.00 16657431.00 15046452.00 2635114.00 9086090.00 9740121.00 207228.00 11455918.00 21468268.00 3424568.00 19992840.00 F 15911221.00 28039338.00 6636354.00 8852655.00 23304136.00 207894.00 9333822.00 9921629.00 2068762.00 1889641.00 10532312.00 6112591.00 G 42865528.00 54144552.00 9263753.00 3489705.00 8983121.00 26659758.00 9661069.00 2656529.00 16874038.00 9223930.00 2341123.00 822074.00 H 2918314.00 4115557.00 10199553.00 3209532.00 220065.00 11204903.00 4102788.00 12360948.00 295147.00 11379835.00 1821829.00 5143926.00