Load time: 09/03/2010 10:15:25 AM Read time: 09/03/2010 10:15:25 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1025 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 29613826 cps Min counts: 171182 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9517804.00 5526831.00 6985312.00 15847965.00 2544527.00 1762955.00 2224190.00 4662649.00 1642674.00 3990725.00 8170600.00 5362820.00 B 9043958.00 3534500.00 15396188.00 2992939.00 8423581.00 4745707.00 10964961.00 18261914.00 3337762.00 1260224.00 6349988.00 4970070.00 C 1329630.00 9059942.00 3110450.00 6042232.00 5604838.00 10193050.00 6153219.00 8779342.00 7321327.00 9967784.00 4373194.00 14582699.00 D 4605348.00 7423308.00 5649251.00 2661514.00 6959415.00 6777576.00 29613826.00 6783009.00 6392576.00 11449849.00 8730953.00 1297556.00 E 12350987.00 3629433.00 21750112.00 9472044.00 22168274.00 3596271.00 12580326.00 10170433.00 6205462.00 2402497.00 7887276.00 1902789.00 F 7884374.00 1836472.00 4285316.00 4654764.00 12991890.00 11544544.00 3954731.00 342364.00 3668951.00 1303517.00 17548830.00 10806426.00 G 10018241.00 6427256.00 12024546.00 22540986.00 16724173.00 2001318.00 12045619.00 5487972.00 7569020.00 1695241.00 7261158.00 17907126.00 H 4910987.00 3633077.00 5645380.00 8091358.00 15291592.00 16515710.00 4766592.00 6125464.00 6029503.00 868472.00 3340905.00 14797112.00 Load time: 09/03/2010 10:15:25 AM Read time: 09/03/2010 10:15:25 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1025 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 29613826 cps Min counts: 171182 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9517804.00 5526831.00 6985312.00 15847965.00 2544527.00 1762955.00 2224190.00 4662649.00 1642674.00 3990725.00 8170600.00 5362820.00 B 9043958.00 3534500.00 15396188.00 2992939.00 8423581.00 4745707.00 10964961.00 18261914.00 3337762.00 1260224.00 6349988.00 4970070.00 C 1329630.00 9059942.00 3110450.00 6042232.00 5604838.00 10193050.00 6153219.00 8779342.00 7321327.00 9967784.00 4373194.00 14582699.00 D 4605348.00 7423308.00 5649251.00 2661514.00 6959415.00 6777576.00 29613826.00 6783009.00 6392576.00 11449849.00 8730953.00 1297556.00 E 12350987.00 3629433.00 21750112.00 9472044.00 22168274.00 3596271.00 12580326.00 10170433.00 6205462.00 2402497.00 7887276.00 1902789.00 F 7884374.00 1836472.00 4285316.00 4654764.00 12991890.00 11544544.00 3954731.00 342364.00 3668951.00 1303517.00 17548830.00 10806426.00 G 10018241.00 6427256.00 12024546.00 22540986.00 16724173.00 2001318.00 12045619.00 5487972.00 7569020.00 1695241.00 7261158.00 17907126.00 H 4910987.00 3633077.00 5645380.00 8091358.00 15291592.00 16515710.00 4766592.00 6125464.00 6029503.00 868472.00 3340905.00 14797112.00