Load time: 09/03/2010 10:17:51 AM Read time: 09/03/2010 10:17:51 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1026 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 92364008 cps Min counts: 100890 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1317898.00 1453758.00 3329845.00 2476304.00 19409868.00 4025588.00 3694359.00 7527044.00 11008391.00 92051224.00 92364008.00 91889680.00 B 1525430.00 7702266.00 47254764.00 4803966.00 11169146.00 6197713.00 17550132.00 1537392.00 201780.00 57855932.00 56081296.00 54796768.00 C 4605221.00 6700678.00 7246225.00 5530890.00 18915906.00 3721450.00 2299778.00 3647016.00 208466.00 31835034.00 31649962.00 30572756.00 D 3806870.00 3041094.00 7486695.00 6893884.00 11251297.00 10878923.00 3721191.00 3774416.00 208028.00 18277616.00 17771856.00 17548080.00 E 2018420.00 9318748.00 2960816.00 4582636.00 1889623.00 1182457.00 1588212.00 1600861.00 207437.00 9847949.00 9908234.00 9662624.00 F 4332433.00 14042962.00 3206864.00 4103135.00 4205329.00 23000050.00 4603172.00 7734881.00 205704.00 6151235.00 6043342.00 6123925.00 G 14162267.00 4637435.00 9244478.00 2791709.00 8784720.00 3990497.00 7969344.00 8891174.00 201780.00 3586651.00 3592772.00 3500999.00 H 5292650.00 6868592.00 10627493.00 11993398.00 4205338.00 1881654.00 8270293.00 1465376.00 204237.00 2198135.00 1986433.00 2190699.00 Load time: 09/03/2010 10:17:51 AM Read time: 09/03/2010 10:17:51 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1026 Comment: T11:primer174 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 92364008 cps Min counts: 100890 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1317898.00 1453758.00 3329845.00 2476304.00 19409868.00 4025588.00 3694359.00 7527044.00 11008391.00 92051224.00 92364008.00 91889680.00 B 1525430.00 7702266.00 47254764.00 4803966.00 11169146.00 6197713.00 17550132.00 1537392.00 201780.00 57855932.00 56081296.00 54796768.00 C 4605221.00 6700678.00 7246225.00 5530890.00 18915906.00 3721450.00 2299778.00 3647016.00 208466.00 31835034.00 31649962.00 30572756.00 D 3806870.00 3041094.00 7486695.00 6893884.00 11251297.00 10878923.00 3721191.00 3774416.00 208028.00 18277616.00 17771856.00 17548080.00 E 2018420.00 9318748.00 2960816.00 4582636.00 1889623.00 1182457.00 1588212.00 1600861.00 207437.00 9847949.00 9908234.00 9662624.00 F 4332433.00 14042962.00 3206864.00 4103135.00 4205329.00 23000050.00 4603172.00 7734881.00 205704.00 6151235.00 6043342.00 6123925.00 G 14162267.00 4637435.00 9244478.00 2791709.00 8784720.00 3990497.00 7969344.00 8891174.00 201780.00 3586651.00 3592772.00 3500999.00 H 5292650.00 6868592.00 10627493.00 11993398.00 4205338.00 1881654.00 8270293.00 1465376.00 204237.00 2198135.00 1986433.00 2190699.00