Load time: 09/20/2010 08:40:05 AM Read time: 09/20/2010 08:40:05 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1045 Comment: Cowpea Plate 4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 75655816 cps Min counts: 64214 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 65825436.00 75655816.00 67302608.00 3796656.00 584437.00 868082.00 495502.00 347030.00 1348914.00 128695.00 B 42138500.00 52039044.00 42549776.00 2749953.00 862500.00 1294826.00 842381.00 950939.00 937069.00 131782.00 C 24760114.00 27013350.00 23444372.00 4548349.00 916493.00 835979.00 588800.00 715571.00 678858.00 128429.00 D 14444715.00 15598512.00 14154573.00 4473142.00 923561.00 734985.00 874083.00 1039302.00 476965.00 130410.00 E 8932395.00 9417273.00 8756107.00 1145326.00 569043.00 1154375.00 2112454.00 1589054.00 426153.00 128886.00 F 5144848.00 5392724.00 5215862.00 6366118.00 1309839.00 556449.00 1411081.00 1586414.00 1174399.00 130905.00 G 2932694.00 3573274.00 2964687.00 6546446.00 768250.00 491692.00 785549.00 1424532.00 1270401.00 130277.00 H 1922051.00 2042162.00 2027792.00 7110775.00 787416.00 782481.00 799133.00 1844369.00 923561.00 130982.00 Load time: 09/20/2010 08:40:05 AM Read time: 09/20/2010 08:40:05 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1045 Comment: Cowpea Plate 4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 75655816 cps Min counts: 64214 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 65825436.00 75655816.00 67302608.00 3796656.00 584437.00 868082.00 495502.00 347030.00 1348914.00 128695.00 B 42138500.00 52039044.00 42549776.00 2749953.00 862500.00 1294826.00 842381.00 950939.00 937069.00 131782.00 C 24760114.00 27013350.00 23444372.00 4548349.00 916493.00 835979.00 588800.00 715571.00 678858.00 128429.00 D 14444715.00 15598512.00 14154573.00 4473142.00 923561.00 734985.00 874083.00 1039302.00 476965.00 130410.00 E 8932395.00 9417273.00 8756107.00 1145326.00 569043.00 1154375.00 2112454.00 1589054.00 426153.00 128886.00 F 5144848.00 5392724.00 5215862.00 6366118.00 1309839.00 556449.00 1411081.00 1586414.00 1174399.00 130905.00 G 2932694.00 3573274.00 2964687.00 6546446.00 768250.00 491692.00 785549.00 1424532.00 1270401.00 130277.00 H 1922051.00 2042162.00 2027792.00 7110775.00 787416.00 782481.00 799133.00 1844369.00 923561.00 130982.00