Load time: 09/30/2010 01:29:04 PM Read time: 09/30/2010 01:29:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1061 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 53141460 cps Min counts: 104751 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 49826444.00 53141460.00 46359636.00 757408.00 788542.00 520826.00 476000.00 1015736.00 1045137.00 209503.00 B 31040868.00 29391136.00 30276462.00 773156.00 1284099.00 929436.00 812250.00 1400600.00 843193.00 214054.00 C 21676278.00 19887890.00 20424188.00 629234.00 1322869.00 807870.00 486588.00 1328182.00 512009.00 216530.00 D 12705671.00 11336737.00 12217912.00 613391.00 673755.00 1118964.00 939681.00 1531459.00 1415148.00 216111.00 E 7365359.00 6815266.00 7082964.00 566547.00 780811.00 735585.00 735566.00 836376.00 251549.00 215216.00 F 4327287.00 4407631.00 4557986.00 845478.00 551141.00 1188831.00 628720.00 1421642.00 1033712.00 218396.00 G 2407702.00 2640511.00 2738432.00 755237.00 606307.00 575344.00 491329.00 999226.00 690264.00 219767.00 H 1365829.00 1597286.00 1699970.00 838337.00 1165465.00 829902.00 864064.00 906204.00 1502381.00 220319.00 Load time: 09/30/2010 01:29:04 PM Read time: 09/30/2010 01:29:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1061 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 53141460 cps Min counts: 104751 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 49826444.00 53141460.00 46359636.00 757408.00 788542.00 520826.00 476000.00 1015736.00 1045137.00 209503.00 B 31040868.00 29391136.00 30276462.00 773156.00 1284099.00 929436.00 812250.00 1400600.00 843193.00 214054.00 C 21676278.00 19887890.00 20424188.00 629234.00 1322869.00 807870.00 486588.00 1328182.00 512009.00 216530.00 D 12705671.00 11336737.00 12217912.00 613391.00 673755.00 1118964.00 939681.00 1531459.00 1415148.00 216111.00 E 7365359.00 6815266.00 7082964.00 566547.00 780811.00 735585.00 735566.00 836376.00 251549.00 215216.00 F 4327287.00 4407631.00 4557986.00 845478.00 551141.00 1188831.00 628720.00 1421642.00 1033712.00 218396.00 G 2407702.00 2640511.00 2738432.00 755237.00 606307.00 575344.00 491329.00 999226.00 690264.00 219767.00 H 1365829.00 1597286.00 1699970.00 838337.00 1165465.00 829902.00 864064.00 906204.00 1502381.00 220319.00 Load time: 09/30/2010 01:31:30 PM Read time: 09/30/2010 01:31:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1062 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32769778 cps Min counts: 4770 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10097851.00 12600129.00 6598712.00 11211004.00 12766856.00 7076736.00 11806684.00 12925596.00 7487912.00 174503.00 174370.00 160241.00 B 28259694.00 10592640.00 13651025.00 27843656.00 10455979.00 12662704.00 28627700.00 11810671.00 14044308.00 171894.00 175341.00 165972.00 C 27584476.00 12024432.00 13310530.00 28856578.00 11812210.00 12843356.00 31047290.00 12801261.00 13857345.00 173532.00 174008.00 169971.00 D 24041190.00 24117460.00 12274034.00 24632648.00 23823102.00 11863683.00 25623800.00 25724284.00 13079841.00 176693.00 175779.00 171952.00 E 26541886.00 24772142.00 10624760.00 26694772.00 22984530.00 10812507.00 27920500.00 23706312.00 11525857.00 178655.00 176750.00 173266.00 F 25115298.00 20809772.00 10507406.00 26195926.00 21182088.00 10731680.00 32769778.00 22028100.00 12605994.00 173761.00 173970.00 172656.00 G 12553000.00 19889154.00 12043928.00 12644393.00 21184964.00 12650689.00 14147684.00 20668056.00 12666303.00 168981.00 171152.00 341333.00 H 20825388.00 20247052.00 12234796.00 21112366.00 22585724.00 12250438.00 24778278.00 20976044.00 12622478.00 165077.00 169152.00 9540.00 Load time: 09/30/2010 01:31:30 PM Read time: 09/30/2010 01:31:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1062 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32769778 cps Min counts: 4770 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10097851.00 12600129.00 6598712.00 11211004.00 12766856.00 7076736.00 11806684.00 12925596.00 7487912.00 174503.00 174370.00 160241.00 B 28259694.00 10592640.00 13651025.00 27843656.00 10455979.00 12662704.00 28627700.00 11810671.00 14044308.00 171894.00 175341.00 165972.00 C 27584476.00 12024432.00 13310530.00 28856578.00 11812210.00 12843356.00 31047290.00 12801261.00 13857345.00 173532.00 174008.00 169971.00 D 24041190.00 24117460.00 12274034.00 24632648.00 23823102.00 11863683.00 25623800.00 25724284.00 13079841.00 176693.00 175779.00 171952.00 E 26541886.00 24772142.00 10624760.00 26694772.00 22984530.00 10812507.00 27920500.00 23706312.00 11525857.00 178655.00 176750.00 173266.00 F 25115298.00 20809772.00 10507406.00 26195926.00 21182088.00 10731680.00 32769778.00 22028100.00 12605994.00 173761.00 173970.00 172656.00 G 12553000.00 19889154.00 12043928.00 12644393.00 21184964.00 12650689.00 14147684.00 20668056.00 12666303.00 168981.00 171152.00 341333.00 H 20825388.00 20247052.00 12234796.00 21112366.00 22585724.00 12250438.00 24778278.00 20976044.00 12622478.00 165077.00 169152.00 9540.00 Load time: 10/01/2010 09:30:17 AM Read time: 10/01/2010 09:30:17 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1064 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 28250018 cps Min counts: 9979 counts Well list: r0(a1:h6) = cps(f2(a1:h6)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27689376.00 19905906.00 28250018.00 10376867.00 4532266.00 2312050.00 B 3929927.00 2230489.00 3100500.00 16546622.00 7155803.00 3386365.00 C 677934.00 331777.00 579949.00 19213468.00 8483390.00 3881257.00 D 144492.00 85663.00 124742.00 18199288.00 7935087.00 3926086.00 E 26833.00 49744.00 38394.00 16380638.00 7010358.00 3307362.00 F 42964.00 24262.00 27862.00 16704762.00 6986606.00 3327597.00 G 19958.00 21082.00 23482.00 18218202.00 7494496.00 3443236.00 H 23158.00 50373.00 20492.00 15201669.00 6296777.00 3157648.00