Load time: 10/08/2010 10:01:56 AM Read time: 10/08/2010 10:01:56 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1082 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 95086088 cps Min counts: 109090 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 45860812.00 95086088.00 94881256.00 862235.00 1431107.00 517177.00 932202.00 2928627.00 2403922.00 219874.00 B 24749706.00 27193894.00 28466458.00 613182.00 229467.00 1199603.00 1169987.00 1751112.00 2582698.00 219912.00 C 17942710.00 17286732.00 18307470.00 678238.00 611507.00 2333501.00 2708063.00 1650161.00 2356865.00 220674.00 D 10346254.00 10145358.00 10457795.00 805915.00 981879.00 2532141.00 884199.00 2350213.00 479605.00 219665.00 E 6530695.00 6116220.00 6222476.00 986866.00 818819.00 1273110.00 1403242.00 1271169.00 1712417.00 222767.00 F 3987355.00 3548652.00 3702615.00 453567.00 1945117.00 2515888.00 1125163.00 1379260.00 1676250.00 219018.00 G 2373448.00 2295023.00 2303558.00 390414.00 1428956.00 1287633.00 1031956.00 1443060.00 2243784.00 218180.00 H 1263898.00 1294238.00 1256132.00 1523039.00 1340070.00 722910.00 1937118.00 1955647.00 1637542.00 218732.00 Load time: 10/08/2010 10:01:56 AM Read time: 10/08/2010 10:01:56 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1082 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 95086088 cps Min counts: 109090 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 45860812.00 95086088.00 94881256.00 862235.00 1431107.00 517177.00 932202.00 2928627.00 2403922.00 219874.00 B 24749706.00 27193894.00 28466458.00 613182.00 229467.00 1199603.00 1169987.00 1751112.00 2582698.00 219912.00 C 17942710.00 17286732.00 18307470.00 678238.00 611507.00 2333501.00 2708063.00 1650161.00 2356865.00 220674.00 D 10346254.00 10145358.00 10457795.00 805915.00 981879.00 2532141.00 884199.00 2350213.00 479605.00 219665.00 E 6530695.00 6116220.00 6222476.00 986866.00 818819.00 1273110.00 1403242.00 1271169.00 1712417.00 222767.00 F 3987355.00 3548652.00 3702615.00 453567.00 1945117.00 2515888.00 1125163.00 1379260.00 1676250.00 219018.00 G 2373448.00 2295023.00 2303558.00 390414.00 1428956.00 1287633.00 1031956.00 1443060.00 2243784.00 218180.00 H 1263898.00 1294238.00 1256132.00 1523039.00 1340070.00 722910.00 1937118.00 1955647.00 1637542.00 218732.00