Load time: 10/15/2010 08:35:19 AM Read time: 10/15/2010 08:35:19 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1101 Comment: Cowpea P#3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 72385800 cps Min counts: 65082 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 72385800.00 69808984.00 66834376.00 446688.00 1256518.00 550165.00 1173485.00 1533657.00 1701561.00 130165.00 B 48196356.00 45040784.00 43862064.00 270190.00 682824.00 695216.00 962894.00 868554.00 2248414.00 133762.00 C 28365804.00 29337264.00 30663152.00 745337.00 471453.00 936416.00 1265160.00 633922.00 1383847.00 138712.00 D 26326422.00 16760531.00 16283368.00 2051986.00 1309170.00 1539082.00 1210604.00 838554.00 2416166.00 137988.00 E 10442989.00 11169477.00 9681522.00 566517.00 1174722.00 985375.00 1570208.00 1960655.00 1530554.00 139797.00 F 5819651.00 6016492.00 7000378.00 1056245.00 698300.00 1775519.00 971822.00 1920298.00 4043920.00 142043.00 G 3931049.00 3656946.00 3868672.00 1346804.00 1091232.00 771511.00 1421575.00 1231315.00 2225599.00 144651.00 H 2227562.00 2537319.00 3396131.00 853345.00 1286861.00 865832.00 1627956.00 1128713.00 2593400.00 145755.00 Load time: 10/15/2010 08:35:19 AM Read time: 10/15/2010 08:35:19 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1101 Comment: Cowpea P#3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 72385800 cps Min counts: 65082 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 72385800.00 69808984.00 66834376.00 446688.00 1256518.00 550165.00 1173485.00 1533657.00 1701561.00 130165.00 B 48196356.00 45040784.00 43862064.00 270190.00 682824.00 695216.00 962894.00 868554.00 2248414.00 133762.00 C 28365804.00 29337264.00 30663152.00 745337.00 471453.00 936416.00 1265160.00 633922.00 1383847.00 138712.00 D 26326422.00 16760531.00 16283368.00 2051986.00 1309170.00 1539082.00 1210604.00 838554.00 2416166.00 137988.00 E 10442989.00 11169477.00 9681522.00 566517.00 1174722.00 985375.00 1570208.00 1960655.00 1530554.00 139797.00 F 5819651.00 6016492.00 7000378.00 1056245.00 698300.00 1775519.00 971822.00 1920298.00 4043920.00 142043.00 G 3931049.00 3656946.00 3868672.00 1346804.00 1091232.00 771511.00 1421575.00 1231315.00 2225599.00 144651.00 H 2227562.00 2537319.00 3396131.00 853345.00 1286861.00 865832.00 1627956.00 1128713.00 2593400.00 145755.00