Load time: 11/01/2010 08:42:04 AM Read time: 11/01/2010 08:42:04 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1131 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 70662320 cps Min counts: 885 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 47929932.00 52876936.00 52920000.00 273078.00 70308672.00 64762400.00 70662320.00 2036.00 2036.00 1884.00 272736.00 271156.00 B 33487280.00 31793738.00 30008504.00 271422.00 120797.00 126983.00 127002.00 1884.00 1979.00 1865.00 274887.00 273440.00 C 18896638.00 19167000.00 17741030.00 270033.00 119559.00 126450.00 127821.00 1808.00 2017.00 1808.00 275591.00 275610.00 D 12914163.00 11015025.00 11166375.00 271441.00 121634.00 127649.00 122681.00 1979.00 1808.00 1865.00 278789.00 275705.00 E 6670976.00 6557517.00 6345664.00 271213.00 124033.00 125708.00 127764.00 1922.00 1960.00 1827.00 277799.00 274297.00 F 4308048.00 4086431.00 4153576.00 273269.00 126012.00 128163.00 125784.00 2093.00 1941.00 1846.00 280445.00 277532.00 G 2793069.00 2533169.00 2547597.00 272012.00 123519.00 125689.00 133379.00 1865.00 2036.00 1846.00 281834.00 273021.00 H 1799892.00 1122790.00 1070158.00 271993.00 1590543.00 1381288.00 1445798.00 1770.00 2150.00 1903.00 275286.00 277038.00 Load time: 11/01/2010 08:42:04 AM Read time: 11/01/2010 08:42:04 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1131 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 70662320 cps Min counts: 885 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 47929932.00 52876936.00 52920000.00 273078.00 70308672.00 64762400.00 70662320.00 2036.00 2036.00 1884.00 272736.00 271156.00 B 33487280.00 31793738.00 30008504.00 271422.00 120797.00 126983.00 127002.00 1884.00 1979.00 1865.00 274887.00 273440.00 C 18896638.00 19167000.00 17741030.00 270033.00 119559.00 126450.00 127821.00 1808.00 2017.00 1808.00 275591.00 275610.00 D 12914163.00 11015025.00 11166375.00 271441.00 121634.00 127649.00 122681.00 1979.00 1808.00 1865.00 278789.00 275705.00 E 6670976.00 6557517.00 6345664.00 271213.00 124033.00 125708.00 127764.00 1922.00 1960.00 1827.00 277799.00 274297.00 F 4308048.00 4086431.00 4153576.00 273269.00 126012.00 128163.00 125784.00 2093.00 1941.00 1846.00 280445.00 277532.00 G 2793069.00 2533169.00 2547597.00 272012.00 123519.00 125689.00 133379.00 1865.00 2036.00 1846.00 281834.00 273021.00 H 1799892.00 1122790.00 1070158.00 271993.00 1590543.00 1381288.00 1445798.00 1770.00 2150.00 1903.00 275286.00 277038.00 Load time: 11/01/2010 08:45:10 AM Read time: 11/01/2010 08:45:10 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1132 Comment: synxnatplate4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 2711829 cps Min counts: 154737 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1560531.00 1243054.00 1197712.00 835872.00 959811.00 2303427.00 1788442.00 578307.00 1425621.00 1364023.00 853670.00 683325.00 B 2057672.00 2236007.00 378457.00 1947092.00 1268047.00 1460855.00 1438907.00 1007056.00 2086139.00 1558124.00 670685.00 1505702.00 C 1895397.00 577318.00 1182123.00 1934323.00 1415437.00 1094903.00 1304214.00 678699.00 1568701.00 1260395.00 325691.00 1649854.00 D 2310647.00 2254282.00 2278476.00 2366689.00 1909082.00 701484.00 1599510.00 309474.00 1453926.00 1477739.00 441406.00 768469.00 E 1394174.00 2200151.00 1715465.00 1587771.00 963998.00 678261.00 2312740.00 1740289.00 1729579.00 1039682.00 734148.00 1099833.00 F 2114365.00 446850.00 1633409.00 1582569.00 573568.00 912660.00 2349457.00 1672776.00 1999958.00 1165105.00 1196056.00 560205.00 G 1831424.00 2563265.00 828601.00 550040.00 1600419.00 1407613.00 709136.00 2135194.00 2261055.00 773247.00 414814.00 951016.00 H 1142967.00 2199755.00 1287692.00 445080.00 598485.00 1822454.00 2711829.00 1778022.00 2166784.00 553733.00 318744.00 426768.00 Load time: 11/01/2010 08:45:10 AM Read time: 11/01/2010 08:45:10 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1132 Comment: synxnatplate4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 2711829 cps Min counts: 154737 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1560531.00 1243054.00 1197712.00 835872.00 959811.00 2303427.00 1788442.00 578307.00 1425621.00 1364023.00 853670.00 683325.00 B 2057672.00 2236007.00 378457.00 1947092.00 1268047.00 1460855.00 1438907.00 1007056.00 2086139.00 1558124.00 670685.00 1505702.00 C 1895397.00 577318.00 1182123.00 1934323.00 1415437.00 1094903.00 1304214.00 678699.00 1568701.00 1260395.00 325691.00 1649854.00 D 2310647.00 2254282.00 2278476.00 2366689.00 1909082.00 701484.00 1599510.00 309474.00 1453926.00 1477739.00 441406.00 768469.00 E 1394174.00 2200151.00 1715465.00 1587771.00 963998.00 678261.00 2312740.00 1740289.00 1729579.00 1039682.00 734148.00 1099833.00 F 2114365.00 446850.00 1633409.00 1582569.00 573568.00 912660.00 2349457.00 1672776.00 1999958.00 1165105.00 1196056.00 560205.00 G 1831424.00 2563265.00 828601.00 550040.00 1600419.00 1407613.00 709136.00 2135194.00 2261055.00 773247.00 414814.00 951016.00 H 1142967.00 2199755.00 1287692.00 445080.00 598485.00 1822454.00 2711829.00 1778022.00 2166784.00 553733.00 318744.00 426768.00