Load time: 05/11/2011 11:54:50 AM Read time: 05/11/2011 11:54:50 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1541 Comment: 1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 50540064 cps Min counts: 57773 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 28996004.00 27596608.00 37560256.00 119048.00 6640893.00 1083422.00 4786744.00 2230204.00 6528379.00 2477200.00 115547.00 2918134.00 B 14362484.00 20483212.00 15541942.00 117374.00 4158603.00 2150987.00 2865353.00 4056728.00 9387100.00 1279470.00 846695.00 2883749.00 C 6692946.00 9621802.00 50540064.00 121179.00 973658.00 1495972.00 1599497.00 1597877.00 2765338.00 3485865.00 345367.00 13715761.00 D 10255366.00 10301007.00 8362568.00 121578.00 861117.00 1685297.00 2357616.00 1406738.00 3451274.00 1257438.00 984637.00 5861478.00 E 3045771.00 4359220.00 2358594.00 119714.00 2495751.00 602947.00 5483554.00 461124.00 8362692.00 14691395.00 124984.00 5295100.00 F 8518036.00 2127642.00 2843697.00 120836.00 4450102.00 4973996.00 1571933.00 1495611.00 4095357.00 123576.00 785125.00 897876.00 G 1757274.00 2174357.00 3557071.00 118610.00 2346110.00 1586558.00 3317221.00 124318.00 11252004.00 2676444.00 128276.00 1142956.00 H 565370.00 451744.00 433270.00 120418.00 4051734.00 1765330.00 1558303.00 1219423.00 126240.00 166500.00 5225928.00 11022017.00 Load time: 05/11/2011 12:51:08 PM Read time: 05/11/2011 12:51:08 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1542 Comment: 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 108486376 cps Min counts: 66754 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 51178640.00 50919488.00 53000732.00 148900.00 12310427.00 2859193.00 8897058.00 3561917.00 5629130.00 2364864.00 3509748.00 12276626.00 B 34290912.00 37365760.00 31946134.00 159993.00 7731123.00 5371527.00 5188128.00 3963663.00 8531416.00 1948264.00 2472209.00 11838467.00 C 21495592.00 20328584.00 24500608.00 155103.00 1566313.00 9548382.00 2869830.00 3273481.00 6063141.00 4267015.00 13046507.00 12686167.00 D 16104138.00 33398158.00 14688765.00 159517.00 1771899.00 3292813.00 5884785.00 3435708.00 7159260.00 2867244.00 5917498.00 100406184.00 E 9498873.00 5115579.00 5064529.00 147454.00 4880677.00 4068794.00 8135971.00 680499.00 8556529.00 16485055.00 5369348.00 108486376.00 F 3454916.00 5101892.00 6183629.00 146865.00 8131030.00 8783991.00 2866861.00 2990999.00 9903897.00 13109737.00 1568048.00 53336988.00 G 2081687.00 2823102.00 2291131.00 152344.00 3815823.00 5033851.00 9997636.00 4351432.00 29267702.00 4697327.00 1941310.00 51713516.00 H 1434935.00 1292979.00 1007373.00 153904.00 6027090.00 3133128.00 6440598.00 2627383.00 33764832.00 3931134.00 13883851.00 133508.00