Plate01 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 y = 326030x - 53074 Temperature(¡C) 1 Standards 3 4 5 6 7 8 9 10 11 12 24.5 58.5 19069800 16868106 8849930 4472351 642006 1737993 3845576 -25201 -25148 -24999 29.25 9357639 16055688 10921948 4456276 7149214 7287796 4659857 -25085 -25095 -25007 14.625 4821518 11292668 13864417 199410 5262342 4369599 3583253 -25218 -25196 -25145 7.3125 2227774 21357488 10811952 215186 8907636 6546059 2270187 -25163 -25158 -25231 3.65625 1110545 14199724 10752282 1918999 7843741 3314778 5330879 -25137 -25187 -25049 1.828125 568625 22597448 9842745 366732 6289568 5676398 724447 -25099 -25067 -25071 0.9140625 267540 22327816 10560754 20473366 5817018 2162026 2499641 -25109 -25102 -25079 0 0 13044051 8959180 87913 5810126 12540712 6594 -25092 -25062 -25172 1 2 3 4 5 6 7 8 9 10 11 12 A 51.9 27.3 13.9 2.1 5.5 12.0 B 49.4 33.7 13.8 22.1 22.5 14.5 C 34.8 42.7 0.8 16.3 13.6 11.2 D 65.7 33.3 0.8 27.5 20.2 7.1 E 43.7 33.1 6.0 24.2 10.3 16.5 F 69.5 30.4 1.3 19.5 17.6 2.4 G 68.6 32.6 63.0 18.0 6.8 7.8 H 40.2 27.6 0.4 18.0 38.6 0.2 Plate02 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 24.5 2397162 1115743 1453463 1199963 1064345 1216284 1710073 877065 1338617 3554083 9943 1458861 1074521 942144 1198995 1006722 2291379 1315784 1285496 875459 1239218 1074805 1284838 1316005 668497 279406 812509 1039376 1310645 1339005 1050946 1151320 990775 3335279 9708 1432382 1930103 1329366 1213680 1318904 1860727 1880355 1729121 805335 938784 5081449 14424 821653 1391854 1341778 667235 1593092 1682600 1394684 1250535 1672237 924105 2758159 9786 1344702 1036992 991547 2014926 1632628 1409560 1805447 1577528 1581887 2318238 3076386 9502 997707 791748 1356005 1705260 988002 1682654 1561926 956629 1628860 1126107 3496252 8644 1397300 1330504 1394646 1479183 783029 1169392 1874506 1442876 410390 1156606 2225173 7516 1261554 "Stock concentrations, raw intensity is 1/2 stock concentration" check with Qubit 1 2 3 4 5 6 7 8 9 10 11 12 A 15.0 7.2 9.2 7.7 6.9 7.8 10.8 5.7 8.5 22.1 0.4 9.3 B 6.9 6.1 7.7 6.5 14.4 8.4 8.2 5.7 7.9 6.9 8.2 8.4 C 4.4 2.0 5.3 6.7 8.4 8.5 6.8 7.4 6.4 20.8 0.4 9.1 D 12.2 8.5 7.8 8.4 11.7 11.9 10.9 5.3 6.1 31.5 0.4 5.4 E 8.9 8.6 4.4 10.1 10.6 8.9 8.0 10.6 6.0 17.2 0.4 8.6 F 6.7 6.4 12.7 10.3 9.0 11.4 10.0 10.0 14.5 19.2 0.4 6.4 G 5.2 8.6 10.8 6.4 10.6 9.9 6.2 10.3 7.2 21.8 0.4 8.9 H 8.5 8.9 9.4 5.1 7.5 11.8 9.2 2.8 7.4 14.0 0.4 8.1