##BLOCKS= 6 Plate: Plate01 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 11740482 -14750 -14575 -14691 -14591 -14750 -14713 -14476 -14756 -14312 -14735 -14902 6146335 -14673 -14697 -14785 -14578 -14702 -14704 -14836 -14820 -14782 -14846 -14876 3298774 -14692 -14778 -14757 -14688 -14441 -14707 -14750 -14796 -14941 -14825 -14855 1557239 -14735 -14710 -14753 -14646 -14624 -14656 -14655 -14776 -14958 -14751 -14869 818689 -14738 -14771 -14651 -14758 -14604 -14740 -14796 -14733 -14850 -14835 -14622 423552 -14631 -14740 -14758 -14603 -14707 -14746 -14674 -14746 -14804 -14844 -14761 215007 -14815 -14672 -14635 -14731 -14795 -14641 -14766 -14846 -14900 -14693 -14749 0 -14835 -14784 -14781 -14704 -14712 -14730 -14547 -14865 -14742 -14678 -14917 ~End Plate: Plate02 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 11627921 1167830 16912 1629941 1891382 807048 871760 3098563 1204 1742 1191 1104 6198721 1209305 1759027 1675152 1370251 1431869 1282278 4010004 1183 1249 1275 1250 3305347 1215353 1776543 1122327 1097312 1358350 1012107 3582543 1242 1186 1275 1265 1573224 985971 875425 1485567 908402 2029529 850140 1259046 1243 1159 1090 1139 838484 953265 1749574 2187667 1244418 1184160 820105 1283710 1261 1192 1146 1349 440071 676007 824023 507346 1312690 869206 12716 1282 1331 1186 1132 1117 234463 762908 1225199 1308094 588726 919622 13281 1323 1206 1277 1239 1294 15354 1027222 504937 719211 774570 777800 1407 1375 1144 1347 1205 1269 ~End Plate: Plate03 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 11555329 1171305 1358802 1570695 1877579 799680 848238 2898076 1082 2766 1297 1189 6156257 1191047 1715588 1662231 1349319 1409044 1260320 3764041 1164 1242 1204 1340 3304886 1193916 1750573 1108685 1083641 1336281 988437 3376770 1105 1278 1228 1070 1580534 966546 863167 1455517 903167 1917502 834030 1179192 1178 1203 1123 1118 842861 934997 1741811 2107220 1240023 1142557 797379 1208699 1219 1126 1156 1476 447137 646324 806073 505033 1302192 848199 11183 1263 1265 1118 1158 1136 234857 749572 1183126 1276730 577027 896182 14880 1441 1075 1150 1268 1243 13046 1021709 489577 702440 788492 760989 1295 1273 1182 1365 1245 1043 ~End Group: Standards Sample Concentration BackCalcConc Wells RFU_Values MeanRFUValue SD CV 01 50.000 49.483 A1 11740482.000 11740482.000 0.000 0.0 02 25.000 25.637 B1 6146335.000 6146335.000 0.000 0.0 03 12.500 13.499 C1 3298774.000 3298774.000 0.000 0.0 04 6.250 6.076 D1 1557239.000 1557239.000 0.000 0.0 05 3.125 2.927 E1 818689.000 818689.000 0.000 0.0 06 1.563 1.243 F1 423552.000 423552.000 0.000 0.0 07 0.781 0.354 G1 215007.000 215007.000 0.000 0.0 Group Summaries ~End Group: Unknowns Sample Wells RFU_Values Concentration Mean_Conc SD CV Dilution AdjConc 101 A2 1171305.000 4.430 4.430 0.000 0.0 1.0 4.430 102 B2 1191047.000 4.515 4.515 0.000 0.0 0.5 2.257 103 C2 1193916.000 4.527 4.527 0.000 0.0 0.3 1.132 104 D2 966546.000 3.558 3.558 0.000 0.0 0.1 0.445 105 E2 934997.000 3.423 3.423 0.000 0.0 0.1 0.214 106 F2 646324.000 2.193 2.193 0.000 0.0 0.0 0.069 107 G2 749572.000 2.633 2.633 0.000 0.0 0.0 0.041 108 H2 1021709.000 3.793 3.793 0.000 0.0 0.0 0.030 109 A3 1358802.000 5.230 5.230 0.000 0.0 0.0 0.020 110 B3 1715588.000 6.751 6.751 0.000 0.0 0.0 0.013 111 C3 1750573.000 6.900 6.900 0.000 0.0 0.0 0.007 112 D3 863167.000 3.117 3.117 0.000 0.0 0.0 0.002 113 E3 1741811.000 6.862 6.862 0.000 0.0 0.0 0.002 114 F3 806073.000 2.874 2.874 0.000 0.0 0.0 0.000 115 G3 1183126.000 4.481 4.481 0.000 0.0 0.0 0.000 116 H3 489577.000 1.525 1.525 0.000 0.0 0.0 0.000 117 A4 1570695.000 6.133 6.133 0.000 0.0 0.0 0.000 118 B4 1662231.000 6.523 6.523 0.000 0.0 0.0 0.000 119 C4 1108685.000 4.164 4.164 0.000 0.0 0.0 0.000 120 D4 1455517.000 5.642 5.642 0.000 0.0 0.0 0.000 121 E4 2107220.000 8.420 8.420 0.000 0.0 0.0 0.000 122 F4 505033.000 1.590 1.590 0.000 0.0 0.0 0.000 123 G4 1276730.000 4.880 4.880 0.000 0.0 0.0 0.000 124 H4 702440.000 2.432 2.432 0.000 0.0 0.0 0.000 125 A5 1877579.000 7.441 7.441 0.000 0.0 0.0 0.000 126 B5 1349319.000 5.189 5.189 0.000 0.0 0.0 0.000 127 C5 1083641.000 4.057 4.057 0.000 0.0 0.0 0.000 128 D5 903167.000 3.287 3.287 0.000 0.0 0.0 0.000 129 E5 1240023.000 4.723 4.723 0.000 0.0 0.0 0.000 130 F5 1302192.000 4.988 4.988 0.000 0.0 0.0 0.000 131 G5 577027.000 1.897 1.897 0.000 0.0 0.0 0.000 132 H5 788492.000 2.799 2.799 0.000 0.0 0.0 0.000 133 A6 799680.000 2.846 2.846 0.000 0.0 0.0 0.000 134 B6 1409044.000 5.444 5.444 0.000 0.0 0.0 0.000 135 C6 1336281.000 5.134 5.134 0.000 0.0 0.0 0.000 136 D6 1917502.000 7.611 7.611 0.000 0.0 0.0 0.000 137 E6 1142557.000 4.308 4.308 0.000 0.0 0.0 0.000 138 F6 848199.000 3.053 3.053 0.000 0.0 0.0 0.000 139 G6 896182.000 3.258 3.258 0.000 0.0 0.0 0.000 140 H6 760989.000 2.681 2.681 0.000 0.0 0.0 0.000 141 A7 848238.000 3.053 3.053 0.000 0.0 0.0 0.000 142 B7 1260320.000 4.810 4.810 0.000 0.0 0.0 0.000 143 C7 988437.000 3.651 3.651 0.000 0.0 0.0 0.000 144 D7 834030.000 2.993 2.993 0.000 0.0 0.0 0.000 145 E7 797379.000 2.837 2.837 0.000 0.0 0.0 0.000 146 F7 11183.000 -0.515 -0.515 0.000 0.0 0.0 -0.000 147 G7 14880.000 -0.499 -0.499 0.000 0.0 0.0 -0.000 148 H7 1295.000 -0.557 -0.557 0.000 0.0 0.0 -0.000 149 A8 2898076.000 11.791 11.791 0.000 0.0 0.0 0.000 150 B8 3764041.000 15.482 15.482 0.000 0.0 0.0 0.000 151 C8 3376770.000 13.831 13.831 0.000 0.0 0.0 0.000 152 D8 1179192.000 4.464 4.464 0.000 0.0 0.0 0.000 153 E8 1208699.000 4.590 4.590 0.000 0.0 0.0 0.000 154 F8 1263.000 -0.557 -0.557 0.000 0.0 0.0 -0.000 155 G8 1441.000 -0.556 -0.556 0.000 0.0 0.0 -0.000 156 H8 1273.000 -0.557 -0.557 0.000 0.0 0.0 -0.000 Group Summaries ~End Group: Unknowns_NoDiln Sample Wells RFU_Values Concentration MeanConc SD CV 01 A2 1167830.000 4.416 4.416 0.000 0.0 02 B2 1209305.000 4.592 4.592 0.000 0.0 03 C2 1215353.000 4.618 4.618 0.000 0.0 04 D2 985971.000 3.640 3.640 0.000 0.0 05 E2 953265.000 3.501 3.501 0.000 0.0 06 F2 676007.000 2.319 2.319 0.000 0.0 07 G2 762908.000 2.690 2.690 0.000 0.0 08 H2 1027222.000 3.816 3.816 0.000 0.0 09 A3 16912.000 -0.490 -0.490 0.000 0.0 10 B3 1759027.000 6.936 6.936 0.000 0.0 11 C3 1776543.000 7.010 7.010 0.000 0.0 12 D3 875425.000 3.169 3.169 0.000 0.0 13 E3 1749574.000 6.895 6.895 0.000 0.0 14 F3 824023.000 2.950 2.950 0.000 0.0 15 G3 1225199.000 4.660 4.660 0.000 0.0 16 H3 504937.000 1.590 1.590 0.000 0.0 17 A4 1629941.000 6.385 6.385 0.000 0.0 18 B4 1675152.000 6.578 6.578 0.000 0.0 19 C4 1122327.000 4.222 4.222 0.000 0.0 20 D4 1485567.000 5.770 5.770 0.000 0.0 21 E4 2187667.000 8.763 8.763 0.000 0.0 22 F4 507346.000 1.600 1.600 0.000 0.0 23 G4 1308094.000 5.014 5.014 0.000 0.0 24 H4 719211.000 2.503 2.503 0.000 0.0 25 A5 1891382.000 7.500 7.500 0.000 0.0 26 B5 1370251.000 5.278 5.278 0.000 0.0 27 C5 1097312.000 4.115 4.115 0.000 0.0 28 D5 908402.000 3.310 3.310 0.000 0.0 29 E5 1244418.000 4.742 4.742 0.000 0.0 30 F5 1312690.000 5.033 5.033 0.000 0.0 31 G5 588726.000 1.947 1.947 0.000 0.0 32 H5 774570.000 2.739 2.739 0.000 0.0 33 A6 807048.000 2.878 2.878 0.000 0.0 34 B6 1431869.000 5.541 5.541 0.000 0.0 35 C6 1358350.000 5.228 5.228 0.000 0.0 36 D6 2029529.000 8.089 8.089 0.000 0.0 37 E6 1184160.000 4.485 4.485 0.000 0.0 38 F6 869206.000 3.143 3.143 0.000 0.0 39 G6 919622.000 3.358 3.358 0.000 0.0 40 H6 777800.000 2.753 2.753 0.000 0.0 41 A7 871760.000 3.154 3.154 0.000 0.0 42 B7 1282278.000 4.903 4.903 0.000 0.0 43 C7 1012107.000 3.752 3.752 0.000 0.0 44 D7 850140.000 3.061 3.061 0.000 0.0 45 E7 820105.000 2.933 2.933 0.000 0.0 46 F7 12716.000 -0.508 -0.508 0.000 0.0 47 G7 13281.000 -0.506 -0.506 0.000 0.0 48 H7 1407.000 -0.556 -0.556 0.000 0.0 49 A8 3098563.000 12.646 12.646 0.000 0.0 50 B8 4010004.000 16.531 16.531 0.000 0.0 51 C8 3582543.000 14.709 14.709 0.000 0.0 52 D8 1259046.000 4.804 4.804 0.000 0.0 53 E8 1283710.000 4.910 4.910 0.000 0.0 54 F8 1282.000 -0.557 -0.557 0.000 0.0 55 G8 1323.000 -0.557 -0.557 0.000 0.0 56 H8 1375.000 -0.556 -0.556 0.000 0.0 Group Summaries ~End Original Filename: Butterfield_HiSeq084; Date Last Saved: 3/26/2015 12:25:20 PM