##BLOCKS= 5 Plate: Plate1 1.3 TimeFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 -144764.79761904763 -144742.79761904763 -144727.79761904763 -144769.79761904763 -144727.79761904763 -144727.79761904763 -144776.79761904763 -144189.79761904763 -144653.79761904763 -144450.79761904763 -144909.79761904763 -144735.79761904763 1004571.2023809524 730429.20238095243 836269.20238095243 644836.20238095243 2786474.2023809524 668865.20238095243 648733.20238095243 465395.20238095237 928623.20238095243 553918.20238095243 520430.20238095237 523646.20238095237 -144417.79761904763 -144645.79761904763 -144368.79761904763 -144648.79761904763 -144606.79761904763 -144798.79761904763 -141118.79761904763 -144800.79761904763 -144759.79761904763 -144813.79761904763 -144617.79761904763 -144049.79761904763 -144673.79761904763 -144643.79761904763 -144812.79761904763 -144731.79761904763 -144753.79761904763 -144716.79761904763 -144689.79761904763 -143368.79761904763 -144427.79761904763 -144791.79761904763 -144747.79761904763 -144909.79761904763 -144785.79761904763 -144749.79761904763 -144732.79761904763 -144695.79761904763 -137940.79761904763 -144368.79761904763 -144703.79761904763 -139862.79761904763 -144823.79761904763 -144642.79761904763 -144667.79761904763 -143734.79761904763 -143850.79761904763 -144418.79761904763 -142652.79761904763 -144510.79761904763 -144710.79761904763 -144597.79761904763 -144736.79761904763 -144738.79761904763 -144114.79761904763 -144775.79761904763 -142622.79761904763 -144767.79761904763 -144101.79761904763 -143929.79761904763 -144762.79761904763 -103955.79761904763 -144769.79761904763 -139194.79761904763 -144426.79761904763 -144736.79761904763 -144797.79761904763 -110965.79761904763 -144753.79761904763 -144493.79761904763 24085811.202380951 18370813.202380951 10755339.202380952 7413118.2023809524 4609660.2023809524 3003821.2023809524 1596534.2023809524 905654.20238095243 615164.20238095243 339506.20238095237 181008.20238095237 -38466.797619047633 ~End Plate: Plate2 1.3 TimeFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 1791641 723932 834293 708936 1106463 3581164 2572038 283013 43171 7340049 376852 868645 1639784 2455854 2882268 49525 883359 4161825 3520876 182034 26097 1679734 36320 8385 1354303 1523616 2269547 1019741 660279 4532425 2986614 271058 289497 1178157 51108 310095 953669 1840929 818863 1698134 910501 4190100 5472930 164062 210946 2139752 64620 507459 1296040 1767002 740059 498505 12484590 6865154 3927383 236982 397296 309312 189786 141812 846547 1165734 1668761 1928445 6249410 4274091 2610644 76385 133055 464967 199477 -4105 1186867 1746091 17593 1084653 6582915 4923278 3390841 46809 203396 303145 201012 4105 543234 1059525 1109303 1095410 4803821 5337941 3454833 40817 51293 210588 3786 1659759 ~End Plate: Plate3 1.3 TimeFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 1684155.5 689322.5 794955.5 677814.5 1048488.5 3456883.5 2491782.5 276561.5 34616.5 7131155.5 362097.5 843773.5 1545026.5 2343763.5 2804632.5 42446.5 822297.5 3934052.5 3330238.5 171119.5 23335.5 1626646.5 32911.5 7889.5 1289969.5 1454042.5 2193277.5 954317.5 611255.5 4281845.5 2827982.5 254514.5 282892.5 1125373.5 43116.5 297180.5 918968.5 1735027.5 744076.5 1586690.5 839292.5 3891262.5 5140852.5 150822.5 278020.5 2051042.5 53159.5 466342.5 1200323.5 1686882.5 679055.5 452584.5 11581613.5 6347586.5 3693514.5 214519.5 368988.5 291312.5 176070.5 135785.5 814706.5 1069927.5 1541645.5 1898368.5 5911435.5 3951077.5 2413460.5 66173.5 121377.5 441122.5 192489.5 -3520.5 1076855.5 1614746.5 13994.5 1022863.5 6143736.5 4550445.5 3165694.5 40682.5 188590.5 275821.5 184641.5 3520.5 476472.5 967924.5 1022094.5 993090.5 4586907.5 5057010.5 3268147.5 33799.5 45462.5 192806.5 2383.5 1563268.5 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 300.000 287.974 H1 24085811.202 24085811.202 0.000 0.0 02 200.000 220.452 H2 18370813.202 18370813.202 0.000 0.0 03 133.333 130.476 H3 10755339.202 10755339.202 0.000 0.0 04 88.889 90.988 H4 7413118.202 7413118.202 0.000 0.0 05 59.259 57.866 H5 4609660.202 4609660.202 0.000 0.0 06 39.506 38.893 H6 3003821.202 3003821.202 0.000 0.0 07 26.337 22.266 H7 1596534.202 1596534.202 0.000 0.0 08 17.558 14.104 H8 905654.202 905654.202 0.000 0.0 09 11.706 10.671 H9 615164.202 615164.202 0.000 0.0 10 7.804 7.415 H10 339506.202 339506.202 0.000 0.0 11 5.202 5.542 H11 181008.202 181008.202 0.000 0.0 12 0.000 2.949 H12 -38466.798 -38466.798 0.000 0.0 Group Summaries MinStd Smallest standard value: -38466.798 Min(MeanValue) MaxStd Largest standard value: 24085811.202 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 1791641.000 24.571 23.936 0.898 3.8 A1 1684155.500 23.301 02 B1 1639784.000 22.777 22.217 0.792 3.6 B1 1545026.500 21.658 03 C1 1354303.000 19.404 19.024 0.537 2.8 C1 1289969.500 18.644 04 D1 953669.000 14.671 14.466 0.290 2.0 D1 918968.500 14.261 05 E1 1296040.000 18.716 18.150 0.800 4.4 E1 1200323.500 17.585 06 F1 846547.000 13.405 13.217 0.266 2.0 F1 814706.500 13.029 07 G1 1186867.000 17.426 16.776 0.919 5.5 G1 1076855.500 16.126 08 H1 543234.000 9.822 9.427 0.558 5.9 H1 476472.500 9.033 09 A2 723932.000 11.957 11.752 0.289 2.5 A2 689322.500 11.548 10 B2 2455854.000 32.419 31.757 0.936 2.9 B2 2343763.500 31.095 11 C2 1523616.000 21.405 20.994 0.581 2.8 C2 1454042.500 20.583 12 D2 1840929.000 25.154 24.528 0.885 3.6 D2 1735027.500 23.902 13 E2 1767002.000 24.280 23.807 0.669 2.8 E2 1686882.500 23.334 14 F2 1165734.000 17.176 16.610 0.800 4.8 F2 1069927.500 16.044 15 G2 1746091.000 24.033 23.257 1.097 4.7 G2 1614746.500 22.481 16 H2 1059525.000 15.921 15.380 0.765 5.0 H2 967924.500 14.839 17 A3 834293.000 13.260 13.028 0.329 2.5 A3 794955.500 12.796 18 B3 2882268.000 37.457 36.998 0.649 1.8 B3 2804632.500 36.540 19 C3 2269547.000 30.218 29.767 0.637 2.1 C3 2193277.500 29.317 20 D3 818863.000 13.078 12.636 0.625 4.9 D3 744076.500 12.195 21 E3 740059.000 12.147 11.787 0.510 4.3 E3 679055.500 11.426 22 F3 1668761.000 23.120 22.369 1.062 4.7 F3 1541645.500 21.618 23 G3 17593.000 3.611 3.590 0.030 0.8 G3 13994.500 3.569 24 H3 1109303.000 16.510 15.994 0.729 4.6 H3 1022094.500 15.479 25 A4 708936.000 11.779 11.595 0.260 2.2 A4 677814.500 11.412 26 B4 49525.000 3.989 3.947 0.059 1.5 B4 42446.500 3.905 27 C4 1019741.000 15.451 15.065 0.547 3.6 C4 954317.500 14.678 28 D4 1698134.000 23.467 22.808 0.931 4.1 D4 1586690.500 22.150 29 E4 498505.000 9.293 9.022 0.384 4.3 E4 452584.500 8.751 30 F4 1928445.000 26.188 26.010 0.251 1.0 F4 1898368.500 25.832 31 G4 1084653.000 16.218 15.853 0.516 3.3 G4 1022863.500 15.488 32 H4 1095410.000 16.345 15.741 0.855 5.4 H4 993090.500 15.137 33 A5 1106463.000 16.476 16.134 0.484 3.0 A5 1048488.500 15.791 34 B5 883359.000 13.840 13.479 0.510 3.8 B5 822297.500 13.119 35 C5 660279.000 11.204 10.915 0.410 3.8 C5 611255.500 10.625 36 D5 910501.000 14.161 13.740 0.595 4.3 D5 839292.500 13.319 37 E5 12484590.000 150.907 145.573 7.544 5.2 E5 11581613.500 140.238 38 F5 6249410.000 77.239 75.243 2.824 3.8 F5 5911435.500 73.246 39 G5 6582915.000 81.180 78.585 3.669 4.7 G5 6143736.500 75.991 40 H5 4803821.000 60.160 58.878 1.812 3.1 H5 4586907.500 57.597 41 A6 3581164.000 45.714 44.980 1.038 2.3 A6 3456883.500 44.246 42 B6 4161825.000 52.575 51.229 1.903 3.7 B6 3934052.500 49.884 43 C6 4532425.000 56.953 55.473 2.093 3.8 C6 4281845.500 53.993 44 D6 4190100.000 52.909 51.143 2.497 4.9 D6 3891262.500 49.378 45 E6 6865154.000 84.514 81.457 4.324 5.3 E6 6347586.500 78.399 46 F6 4274091.000 53.901 51.993 2.699 5.2 F6 3951077.500 50.085 47 G6 4923278.000 61.571 59.369 3.115 5.2 G6 4550445.500 57.166 48 H6 5337941.000 66.470 64.811 2.347 3.6 H6 5057010.500 63.151 49 A7 2572038.000 33.792 33.318 0.670 2.0 A7 2491782.500 32.843 50 B7 3520876.000 45.002 43.876 1.593 3.6 B7 3330238.500 42.750 51 C7 2986614.000 38.690 37.753 1.325 3.5 C7 2827982.500 36.816 52 D7 5472930.000 68.065 66.103 2.774 4.2 D7 5140852.500 64.142 53 E7 3927383.000 49.805 48.423 1.954 4.0 E7 3693514.500 47.042 54 F7 2610644.000 34.248 33.083 1.647 5.0 F7 2413460.500 31.918 55 G7 3390841.000 43.466 42.136 1.881 4.5 G7 3165694.500 40.806 56 H7 3454833.000 44.222 43.119 1.560 3.6 H7 3268147.500 42.016 57 A8 283013.000 6.747 6.709 0.054 0.8 A8 276561.500 6.671 58 B8 182034.000 5.554 5.490 0.091 1.7 B8 171119.500 5.425 59 C8 271058.000 6.606 6.508 0.138 2.1 C8 254514.500 6.410 60 D8 164062.000 5.342 5.264 0.111 2.1 D8 150822.500 5.185 61 E8 236982.000 6.203 6.071 0.188 3.1 E8 214519.500 5.938 62 F8 76385.000 4.306 4.246 0.085 2.0 F8 66173.500 4.185 63 G8 46809.000 3.956 3.920 0.051 1.3 G8 40682.500 3.884 64 H8 40817.000 3.886 3.844 0.059 1.5 H8 33799.500 3.803 65 A9 43171.000 3.913 3.863 0.071 1.9 A9 34616.500 3.812 66 B9 26097.000 3.712 3.695 0.023 0.6 B9 23335.500 3.679 67 C9 289497.000 6.824 6.785 0.055 0.8 C9 282892.500 6.746 68 D9 210946.000 5.896 6.292 0.560 8.9 D9 278020.500 6.688 69 E9 397296.000 8.097 7.930 0.236 3.0 E9 368988.500 7.763 70 F9 133055.000 4.975 4.906 0.098 2.0 F9 121377.500 4.837 71 G9 203396.000 5.806 5.719 0.124 2.2 G9 188590.500 5.632 72 H9 51293.000 4.009 3.975 0.049 1.2 H9 45462.500 3.941 73 A10 7340049.000 90.125 88.891 1.745 2.0 A10 7131155.500 87.657 74 B10 1679734.000 23.249 22.936 0.444 1.9 B10 1626646.500 22.622 75 C10 1178157.000 17.323 17.011 0.441 2.6 C10 1125373.500 16.699 76 D10 2139752.000 28.684 28.160 0.741 2.6 D10 2051042.500 27.636 77 E10 309312.000 7.058 6.952 0.150 2.2 E10 291312.500 6.845 78 F10 464967.000 8.897 8.756 0.199 2.3 F10 441122.500 8.615 79 G10 303145.000 6.985 6.824 0.228 3.3 G10 275821.500 6.662 80 H10 210588.000 5.891 5.786 0.149 2.6 H10 192806.500 5.681 81 A11 376852.000 7.856 7.769 0.123 1.6 A11 362097.500 7.681 82 B11 36320.000 3.832 3.812 0.028 0.7 B11 32911.500 3.792 83 C11 51108.000 4.007 3.960 0.067 1.7 C11 43116.500 3.913 84 D11 64620.000 4.167 4.099 0.096 2.3 D11 53159.500 4.031 85 E11 189786.000 5.646 5.565 0.115 2.1 E11 176070.500 5.484 86 F11 199477.000 5.760 5.719 0.058 1.0 F11 192489.500 5.678 87 G11 201012.000 5.778 5.682 0.137 2.4 G11 184641.500 5.585 88 H11 3786.000 3.448 3.440 0.012 0.3 H11 2383.500 3.432 89 A12 868645.000 13.666 13.519 0.208 1.5 A12 843773.500 13.372 90 B12 8385.000 3.502 3.500 0.004 0.1 B12 7889.500 3.497 91 C12 310095.000 7.067 6.991 0.108 1.5 C12 297180.500 6.915 92 D12 507459.000 9.399 9.156 0.344 3.8 D12 466342.500 8.913 93 E12 141812.000 5.079 5.043 0.050 1.0 E12 135785.500 5.008 94 H12 1659759.000 23.013 22.443 0.806 3.6 H12 1563268.500 21.873 Group Summaries InRange R - Outside standard range ~End Original Filename: HiSeq1775_HiSeq1817_HiSeq1864_HiSeq1780; Date Last Saved: 6/24/2019 4:23:56 PM