##BLOCKS= 5 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 -1697.9285714285716 -1563.9285714285716 -1568.9285714285716 -1570.9285714285716 -1503.9285714285716 -1527.9285714285716 -1532.9285714285716 -789.92857142857156 -1378.9285714285716 -1186.9285714285716 -1645.9285714285716 -1546.9285714285716 20651294.071428571 13866907.071428571 9455341.07142857 6301144.0714285718 4223207.0714285718 3005517.0714285714 1959046.0714285714 1298416.0714285714 858692.07142857148 573254.07142857148 377177.07142857142 18228.071428571428 -1382.9285714285716 -1564.9285714285716 -1439.9285714285716 -1519.9285714285716 -1509.9285714285716 -1543.9285714285716 2832.0714285714284 -1538.9285714285716 -1471.9285714285716 -1536.9285714285716 -1366.9285714285716 -750.92857142857156 -1455.9285714285716 -1383.9285714285716 -1640.9285714285716 -1604.9285714285716 -1587.9285714285716 -1491.9285714285716 -1443.9285714285716 -1608.9285714285716 -999.92857142857156 -1582.9285714285716 -1536.9285714285716 -1584.9285714285716 -1623.9285714285716 -1525.9285714285716 -1513.9285714285716 -1477.9285714285716 6432.0714285714284 -1101.9285714285716 -1506.9285714285716 9311.0714285714275 -1506.9285714285716 -1473.9285714285716 -1475.9285714285716 -1219.9285714285716 -691.92857142857156 -1275.9285714285716 463.07142857142844 -1397.9285714285716 -1461.9285714285716 -1346.9285714285716 -1399.9285714285716 -1542.9285714285716 -599.92857142857156 -1540.9285714285716 2006.0714285714284 -1612.9285714285716 -1026.9285714285716 -947.92857142857156 -1577.9285714285716 29984.071428571428 -1474.9285714285716 1819.0714285714284 -1270.9285714285716 -1443.9285714285716 -1615.9285714285716 41679.071428571428 -1565.9285714285716 -1307.9285714285716 -1018.9285714285716 -1118.9285714285716 11487.071428571428 -1307.9285714285716 -1506.9285714285716 -1596.9285714285716 -1462.9285714285716 -1412.9285714285716 -1477.9285714285716 -1429.9285714285716 -1546.9285714285716 -1536.9285714285716 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 4380304 2955587 9271117 4459274 4588992 5422634 4164480 4582786 4846476 6580453 5370733 3701015 5392631 3229781 5382648 3992240 3879082 4965173 2746388 2743555 4751744 3690443 3338278 6677330 4200389 3152143 5813893 4914313 2991714 2788688 3785754 6253605 4505320 4165437 3767127 4246472 4024566 3328825 3923659 3785704 3293478 3636668 4294847 3198214 4895565 5613428 3627118 4576509 6218815 4020952 4818562 3561063 3521422 3235071 19787 15940225 4238018 7738186 4455076 15268555 3644938 5656864 4544956 12238813 12916886 6266006 20239 519817 20816590 4500099 8378144 18248862 6384370 8951637 6357805 4208972 5888611 4327505 18423 4300106 3624353 4456690 3445761 4553059 6514668 2859201 5086030 4883545 4396695 5792229 23866 6928184 5997805 10897818 4913933 2578236 ~End Plate: Plate3 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 2280398 1312011 7825662 3216549 4526255 19960380 4089082 3158242 4108745 6632714 9130014 895462 2761096 5307042 5651146 2582327 2481616 2760716 7053980 1336255 4305133 3978646 2468124 1697588 4617148 6032483 4154849 3770532 5109870 4002491 3493616 4340196 4387178 4608146 4666087 4532792 5331114 11750380 3927067 5268036 7516106 10323625 17462 3926627 4833950 6959878 9557273 9725151 3635433 3002591 2857954 3040293 2347587 2649272 2348094 2797608 2968389 3423353 9654610 7316739 4242714 4005719 4143416 3882045 2760929 2624261 3335837 2393839 3241566 2689716 2843355 3138338 3296240 3867742 4164976 4205140 3309012 2984237 4138318 2976748 4276490 3241029 3236714 3585208 3739773 4883577 5039717 6367658 4665638 4125979 7055032 28208 4758942 5219848 3531530 4149411 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 100.000 99.465 B1 20651294.071 20651294.071 0.000 0.0 02 66.667 66.608 B2 13866907.071 13866907.071 0.000 0.0 03 44.444 45.243 B3 9455341.071 9455341.071 0.000 0.0 04 29.630 29.968 B4 6301144.071 6301144.071 0.000 0.0 05 19.753 19.904 B5 4223207.071 4223207.071 0.000 0.0 06 13.169 14.007 B6 3005517.071 3005517.071 0.000 0.0 07 8.779 8.939 B7 1959046.071 1959046.071 0.000 0.0 08 5.853 5.740 B8 1298416.071 1298416.071 0.000 0.0 09 3.902 3.610 B9 858692.071 858692.071 0.000 0.0 10 2.601 2.228 B10 573254.071 573254.071 0.000 0.0 11 1.734 1.278 B11 377177.071 377177.071 0.000 0.0 12 0.000 -0.460 B12 18228.071 18228.071 0.000 0.0 Group Summaries MinStd Smallest standard value: 18228.071 Min(MeanValue) MaxStd Largest standard value: 20651294.071 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 4380304.000 20.665 20.665 0.000 0.0 02 B1 5392631.000 25.568 25.568 0.000 0.0 03 C1 4200389.000 19.794 19.794 0.000 0.0 04 D1 4024566.000 18.942 18.942 0.000 0.0 05 E1 6218815.000 29.569 29.569 0.000 0.0 06 F1 3644938.000 17.104 17.104 0.000 0.0 07 G1 6384370.000 30.371 30.371 0.000 0.0 08 H1 6514668.000 31.002 31.002 0.000 0.0 09 A2 2955587.000 13.765 13.765 0.000 0.0 097 A1 2280398.000 10.496 10.496 0.000 0.0 098 B1 2761096.000 12.824 12.824 0.000 0.0 099 C1 4617148.000 21.812 21.812 0.000 0.0 10 B2 3229781.000 15.093 15.093 0.000 0.0 100 D1 5331114.000 25.270 25.270 0.000 0.0 101 E1 3635433.000 17.058 17.058 0.000 0.0 102 F1 4242714.000 19.999 19.999 0.000 0.0 103 G1 3296240.000 15.415 15.415 0.000 0.0 104 H1 3739773.000 17.563 17.563 0.000 0.0 105 A2 1312011.000 5.806 5.806 0.000 0.0 106 B2 5307042.000 25.153 25.153 0.000 0.0 107 C2 6032483.000 28.667 28.667 0.000 0.0 108 D2 11750380.000 56.358 56.358 0.000 0.0 109 E2 3002591.000 13.993 13.993 0.000 0.0 11 C2 3152143.000 14.717 14.717 0.000 0.0 110 F2 4005719.000 18.851 18.851 0.000 0.0 111 G2 3867742.000 18.183 18.183 0.000 0.0 112 H2 4883577.000 23.103 23.103 0.000 0.0 113 A3 7825662.000 37.351 37.351 0.000 0.0 114 B3 5651146.000 26.820 26.820 0.000 0.0 115 C3 4154849.000 19.573 19.573 0.000 0.0 116 D3 3927067.000 18.470 18.470 0.000 0.0 117 E3 2857954.000 13.293 13.293 0.000 0.0 118 F3 4143416.000 19.518 19.518 0.000 0.0 119 G3 4164976.000 19.622 19.622 0.000 0.0 12 D2 3328825.000 15.573 15.573 0.000 0.0 120 H3 5039717.000 23.859 23.859 0.000 0.0 121 A4 3216549.000 15.029 15.029 0.000 0.0 122 B4 2582327.000 11.958 11.958 0.000 0.0 123 C4 3770532.000 17.712 17.712 0.000 0.0 124 D4 5268036.000 24.965 24.965 0.000 0.0 125 E4 3040293.000 14.176 14.176 0.000 0.0 126 F4 3882045.000 18.252 18.252 0.000 0.0 127 G4 4205140.000 19.817 19.817 0.000 0.0 128 H4 6367658.000 30.290 30.290 0.000 0.0 129 A5 4526255.000 21.372 21.372 0.000 0.0 13 E2 4020952.000 18.925 18.925 0.000 0.0 130 B5 2481616.000 11.470 11.470 0.000 0.0 131 C5 5109870.000 24.199 24.199 0.000 0.0 132 D5 7516106.000 35.852 35.852 0.000 0.0 133 E5 2347587.000 10.821 10.821 0.000 0.0 134 F5 2760929.000 12.823 12.823 0.000 0.0 135 G5 3309012.000 15.477 15.477 0.000 0.0 136 H5 4665638.000 22.047 22.047 0.000 0.0 137 A6 19960380.000 96.119 96.119 0.000 0.0 138 B6 2760716.000 12.822 12.822 0.000 0.0 139 C6 4002491.000 18.836 18.836 0.000 0.0 14 F2 5656864.000 26.848 26.848 0.000 0.0 140 D6 10323625.000 49.448 49.448 0.000 0.0 141 E6 2649272.000 12.282 12.282 0.000 0.0 142 F6 2624261.000 12.161 12.161 0.000 0.0 143 G6 2984237.000 13.904 13.904 0.000 0.0 144 H6 4125979.000 19.434 19.434 0.000 0.0 145 A7 4089082.000 19.255 19.255 0.000 0.0 146 B7 7053980.000 33.614 33.614 0.000 0.0 147 C7 3493616.000 16.371 16.371 0.000 0.0 148 D7 17462.000 R -0.464 -0.464 0.000 0.0 149 E7 2348094.000 10.823 10.823 0.000 0.0 15 G2 8951637.000 42.804 42.804 0.000 0.0 150 F7 3335837.000 15.607 15.607 0.000 0.0 151 G7 4138318.000 19.493 19.493 0.000 0.0 152 H7 7055032.000 33.619 33.619 0.000 0.0 153 A8 3158242.000 14.747 14.747 0.000 0.0 154 B8 1336255.000 5.923 5.923 0.000 0.0 155 C8 4340196.000 20.471 20.471 0.000 0.0 156 D8 3926627.000 18.468 18.468 0.000 0.0 157 E8 2797608.000 13.000 13.000 0.000 0.0 158 F8 2393839.000 11.045 11.045 0.000 0.0 159 G8 2976748.000 13.868 13.868 0.000 0.0 16 H2 2859201.000 13.299 13.299 0.000 0.0 160 H8 28208.000 -0.412 -0.412 0.000 0.0 161 A9 4108745.000 19.350 19.350 0.000 0.0 162 B9 4305133.000 20.301 20.301 0.000 0.0 163 C9 4387178.000 20.699 20.699 0.000 0.0 164 D9 4833950.000 22.862 22.862 0.000 0.0 165 E9 2968389.000 13.827 13.827 0.000 0.0 166 F9 3241566.000 15.150 15.150 0.000 0.0 167 G9 4276490.000 20.163 20.163 0.000 0.0 168 H9 4758942.000 22.499 22.499 0.000 0.0 169 A10 6632714.000 31.574 31.574 0.000 0.0 17 A3 9271117.000 44.351 44.351 0.000 0.0 170 B10 3978646.000 18.720 18.720 0.000 0.0 171 C10 4608146.000 21.769 21.769 0.000 0.0 172 D10 6959878.000 33.158 33.158 0.000 0.0 173 E10 3423353.000 16.031 16.031 0.000 0.0 174 F10 2689716.000 12.478 12.478 0.000 0.0 175 G10 3241029.000 15.148 15.148 0.000 0.0 176 H10 5219848.000 24.731 24.731 0.000 0.0 177 A11 9130014.000 43.668 43.668 0.000 0.0 178 B11 2468124.000 11.405 11.405 0.000 0.0 179 C11 4666087.000 22.049 22.049 0.000 0.0 18 B3 5382648.000 25.520 25.520 0.000 0.0 180 D11 9557273.000 45.737 45.737 0.000 0.0 181 E11 9654610.000 46.208 46.208 0.000 0.0 182 F11 2843355.000 13.222 13.222 0.000 0.0 183 G11 3236714.000 15.127 15.127 0.000 0.0 184 H11 3531530.000 16.555 16.555 0.000 0.0 185 A12 895462.000 3.788 3.788 0.000 0.0 186 B12 1697588.000 7.673 7.673 0.000 0.0 187 C12 4532792.000 21.404 21.404 0.000 0.0 188 D12 9725151.000 46.550 46.550 0.000 0.0 189 E12 7316739.000 34.886 34.886 0.000 0.0 19 C3 5813893.000 27.608 27.608 0.000 0.0 190 F12 3138338.000 14.650 14.650 0.000 0.0 191 G12 3585208.000 16.815 16.815 0.000 0.0 192 H12 4149411.000 19.547 19.547 0.000 0.0 20 D3 3923659.000 18.454 18.454 0.000 0.0 21 E3 4818562.000 22.788 22.788 0.000 0.0 22 F3 4544956.000 21.463 21.463 0.000 0.0 23 G3 6357805.000 30.242 30.242 0.000 0.0 24 H3 5086030.000 24.083 24.083 0.000 0.0 25 A4 4459274.000 21.048 21.048 0.000 0.0 26 B4 3992240.000 18.786 18.786 0.000 0.0 27 C4 4914313.000 23.251 23.251 0.000 0.0 28 D4 3785704.000 17.786 17.786 0.000 0.0 29 E4 3561063.000 16.698 16.698 0.000 0.0 30 F4 12238813.000 58.724 58.724 0.000 0.0 31 G4 4208972.000 19.836 19.836 0.000 0.0 32 H4 4883545.000 23.102 23.102 0.000 0.0 33 A5 4588992.000 21.676 21.676 0.000 0.0 34 B5 3879082.000 18.238 18.238 0.000 0.0 35 C5 2991714.000 13.940 13.940 0.000 0.0 36 D5 3293478.000 15.402 15.402 0.000 0.0 37 E5 3521422.000 16.506 16.506 0.000 0.0 38 F5 12916886.000 62.007 62.007 0.000 0.0 39 G5 5888611.000 27.970 27.970 0.000 0.0 40 H5 4396695.000 20.745 20.745 0.000 0.0 41 A6 5422634.000 25.713 25.713 0.000 0.0 42 B6 4965173.000 23.498 23.498 0.000 0.0 43 C6 2788688.000 12.957 12.957 0.000 0.0 44 D6 3636668.000 17.064 17.064 0.000 0.0 45 E6 3235071.000 15.119 15.119 0.000 0.0 46 F6 6266006.000 29.798 29.798 0.000 0.0 47 G6 4327505.000 20.410 20.410 0.000 0.0 48 H6 5792229.000 27.503 27.503 0.000 0.0 49 A7 4164480.000 19.620 19.620 0.000 0.0 50 B7 2746388.000 12.752 12.752 0.000 0.0 51 C7 3785754.000 17.786 17.786 0.000 0.0 52 D7 4294847.000 20.251 20.251 0.000 0.0 53 E7 19787.000 -0.452 -0.452 0.000 0.0 54 F7 20239.000 -0.450 -0.450 0.000 0.0 55 G7 18423.000 -0.459 -0.459 0.000 0.0 56 H7 23866.000 -0.433 -0.433 0.000 0.0 57 A8 4582786.000 21.646 21.646 0.000 0.0 58 B8 2743555.000 12.739 12.739 0.000 0.0 59 C8 6253605.000 29.738 29.738 0.000 0.0 60 D8 3198214.000 14.940 14.940 0.000 0.0 61 E8 15940225.000 76.649 76.649 0.000 0.0 62 F8 519817.000 1.969 1.969 0.000 0.0 63 G8 4300106.000 20.277 20.277 0.000 0.0 64 H8 6928184.000 33.005 33.005 0.000 0.0 65 A9 4846476.000 22.923 22.923 0.000 0.0 66 B9 4751744.000 22.464 22.464 0.000 0.0 67 C9 4505320.000 21.271 21.271 0.000 0.0 68 D9 4895565.000 23.161 23.161 0.000 0.0 69 E9 4238018.000 19.976 19.976 0.000 0.0 70 F9 20816590.000 R 100.265 100.265 0.000 0.0 71 G9 3624353.000 17.004 17.004 0.000 0.0 72 H9 5997805.000 28.499 28.499 0.000 0.0 73 A10 6580453.000 31.320 31.320 0.000 0.0 74 B10 3690443.000 17.324 17.324 0.000 0.0 75 C10 4165437.000 19.625 19.625 0.000 0.0 76 D10 5613428.000 26.637 26.637 0.000 0.0 77 E10 7738186.000 36.927 36.927 0.000 0.0 78 F10 4500099.000 21.245 21.245 0.000 0.0 79 G10 4456690.000 21.035 21.035 0.000 0.0 80 H10 10897818.000 52.229 52.229 0.000 0.0 81 A11 5370733.000 25.462 25.462 0.000 0.0 82 B11 3338278.000 15.619 15.619 0.000 0.0 83 C11 3767127.000 17.696 17.696 0.000 0.0 84 D11 3627118.000 17.018 17.018 0.000 0.0 85 E11 4455076.000 21.027 21.027 0.000 0.0 86 F11 8378144.000 40.027 40.027 0.000 0.0 87 G11 3445761.000 16.139 16.139 0.000 0.0 88 H11 4913933.000 23.250 23.250 0.000 0.0 89 A12 3701015.000 17.376 17.376 0.000 0.0 90 B12 6677330.000 31.790 31.790 0.000 0.0 91 C12 4246472.000 20.017 20.017 0.000 0.0 92 D12 4576509.000 21.615 21.615 0.000 0.0 93 E12 15268555.000 73.396 73.396 0.000 0.0 94 F12 18248862.000 87.830 87.830 0.000 0.0 95 G12 4553059.000 21.502 21.502 0.000 0.0 96 H12 2578236.000 11.938 11.938 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: NovaSeq024_02-19-2019; Date Last Saved: 2/19/2019 11:59:16 AM