Load time: 10/24/2008 10:21:24 AM Read time: 10/24/2008 10:21:24 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: DNATech Fluorescence Int Plate ID: #1718 Comment: new comment Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 32.2 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f1 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 4 A/D converter gain: x1 Integrating gain: x1 Max cps: 236030 cps Min counts: 624 counts Well list: r0(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a1,a7,b1) = (f1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a1,a7,b1)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 859500.00 691600.00 669700.00 698300.00 724600.00 710300.00 624400.00 1132300.00 636200.00 692800.00 1054100.00 750700.00 B 767300.00 756400.00 628900.00 659600.00 706100.00 722500.00 10990500.00 772100.00 769200.00 669500.00 1036400.00 711500.00 C 699800.00 972700.00 1165600.00 734500.00 684400.00 641100.00 751300.00 751300.00 1611000.00 14426300.00 914600.00 4205000.00 D 1127500.00 753200.00 767700.00 754700.00 955800.00 860100.00 693000.00 1025700.00 867000.00 23603000.00 853100.00 1217400.00 E 1216300.00 761600.00 1027400.00 754700.00 762900.00 978800.00 3797000.00 731700.00 725400.00 808500.00 1187700.00 762000.00 F 1143500.00 671100.00 774200.00 1047100.00 871900.00 1708000.00 863200.00 844500.00 766000.00 778900.00 709200.00 720400.00 G 883400.00 862600.00 635200.00 696000.00 904100.00 1620100.00 741600.00 710900.00 834000.00 688000.00 775500.00 708400.00 H 937900.00 678500.00 693700.00 722900.00 728600.00 750900.00 751100.00 759900.00 804700.00 699100.00 714900.00 941900.00 Well list: r1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) = (f1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) - avg(f1(a1,a7,b1))) Data: BACKGROUND SUBTRACTED DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A -58800.00 -80700.00 -52100.00 -25800.00 -40100.00 381900.00 -114200.00 -57600.00 303700.00 300.00 B 6000.00 -121500.00 -90800.00 -44300.00 -27900.00 10240100.00 21700.00 18800.00 -80900.00 286000.00 -38900.00 C -50600.00 222300.00 415200.00 -15900.00 -66000.00 -109300.00 900.00 900.00 860600.00 13675900.00 164200.00 3454600.00 D 377100.00 2800.00 17300.00 4300.00 205400.00 109700.00 -57400.00 275300.00 116600.00 22852600.00 102700.00 467000.00 E 465900.00 11200.00 277000.00 4300.00 12500.00 228400.00 3046600.00 -18700.00 -25000.00 58100.00 437300.00 11600.00 F 393100.00 -79300.00 23800.00 296700.00 121500.00 957600.00 112800.00 94100.00 15600.00 28500.00 -41200.00 -30000.00 G 133000.00 112200.00 -115200.00 -54400.00 153700.00 869700.00 -8800.00 -39500.00 83600.00 -62400.00 25100.00 -42000.00 H 187500.00 -71900.00 -56700.00 -27500.00 -21800.00 500.00 700.00 9500.00 54300.00 -51300.00 -35500.00 191500.00 Load time: 10/24/2008 10:48:43 AM Read time: 10/24/2008 10:48:43 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: DNATech Fluorescence Int Plate ID: #1719 Comment: new comment Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 32.3 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f1 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 4 A/D converter gain: x1 Integrating gain: x1 Max cps: 228951 cps Min counts: 631 counts Well list: r0(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a1,a7,b1) = (f1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a1,a7,b1)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 861600.00 702700.00 746300.00 736200.00 723500.00 669200.00 712000.00 1230600.00 748400.00 765200.00 1022900.00 672200.00 B 773600.00 785200.00 644200.00 735900.00 735900.00 742900.00 10995200.00 730700.00 710700.00 687100.00 1009100.00 774900.00 C 691600.00 1003400.00 1171700.00 769800.00 796300.00 699500.00 771300.00 797200.00 1616300.00 14170400.00 960400.00 4117800.00 D 1089000.00 717400.00 762900.00 723300.00 851000.00 833600.00 699500.00 1052600.00 796300.00 22895100.00 814600.00 1237600.00 E 1183500.00 791300.00 1033700.00 830800.00 829200.00 896500.00 3665500.00 786400.00 745400.00 871700.00 1232500.00 769000.00 F 1060000.00 648400.00 856900.00 1119500.00 863200.00 1672900.00 859900.00 791100.00 807100.00 778900.00 690500.00 674700.00 G 804500.00 791700.00 631800.00 710100.00 790400.00 1522700.00 692200.00 711300.00 751100.00 761800.00 756100.00 722100.00 H 915400.00 705000.00 637900.00 708400.00 743500.00 719300.00 720600.00 814200.00 804500.00 653900.00 663100.00 980400.00 Well list: r1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) = (f1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) - avg(f1(a1,a7,b1))) Data: BACKGROUND SUBTRACTED DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A -79700.00 -36100.00 -46200.00 -58900.00 -113200.00 448200.00 -34000.00 -17200.00 240500.00 -110200.00 B 2800.00 -138200.00 -46500.00 -46500.00 -39500.00 10212800.00 -51700.00 -71700.00 -95300.00 226700.00 -7500.00 C -90800.00 221000.00 389300.00 -12600.00 13900.00 -82900.00 -11100.00 14800.00 833900.00 13388000.00 178000.00 3335400.00 D 306600.00 -65000.00 -19500.00 -59100.00 68600.00 51200.00 -82900.00 270200.00 13900.00 22112700.00 32200.00 455200.00 E 401100.00 8900.00 251300.00 48400.00 46800.00 114100.00 2883100.00 4000.00 -37000.00 89300.00 450100.00 -13400.00 F 277600.00 -134000.00 74500.00 337100.00 80800.00 890500.00 77500.00 8700.00 24700.00 -3500.00 -91900.00 -107700.00 G 22100.00 9300.00 -150600.00 -72300.00 8000.00 740300.00 -90200.00 -71100.00 -31300.00 -20600.00 -26300.00 -60300.00 H 133000.00 -77400.00 -144500.00 -74000.00 -38900.00 -63100.00 -61800.00 31800.00 22100.00 -128500.00 -119300.00 198000.00 Load time: 10/24/2008 10:50:37 AM Read time: 10/24/2008 10:50:37 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: DNATech Fluorescence Int Plate ID: #1720 Comment: new comment Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 32.3 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f1 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 4 A/D converter gain: x1 Integrating gain: x1 Max cps: 224331 cps Min counts: 611 counts Well list: r0(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a1,a7,b1) = (f1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a1,a7,b1)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 863500.00 719500.00 714500.00 704000.00 740200.00 655400.00 683600.00 1174600.00 777200.00 712000.00 982800.00 714100.00 B 745800.00 757000.00 634100.00 698700.00 706500.00 756800.00 10589500.00 774700.00 765200.00 669200.00 1019400.00 754500.00 C 666900.00 923400.00 1128800.00 796500.00 667800.00 715100.00 783300.00 714100.00 1613800.00 14279500.00 953700.00 3934700.00 D 1065700.00 764100.00 783500.00 812500.00 892300.00 842800.00 683100.00 1053700.00 801800.00 22433100.00 828700.00 1208700.00 E 1115700.00 722700.00 997300.00 838600.00 774200.00 959800.00 3777400.00 770200.00 726700.00 824700.00 1231900.00 736600.00 F 1174400.00 729200.00 807900.00 1092000.00 871200.00 1682300.00 876300.00 848700.00 756400.00 801200.00 747900.00 721000.00 G 775500.00 770000.00 611800.00 652000.00 818400.00 1615600.00 702900.00 699100.00 699800.00 674300.00 721800.00 761200.00 H 900500.00 611400.00 674700.00 631200.00 671600.00 745400.00 784100.00 769200.00 760400.00 639400.00 701000.00 991600.00 Well list: r1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) = (f1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) - avg(f1(a1,a7,b1))) Data: BACKGROUND SUBTRACTED DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A -44800.00 -49800.00 -60300.00 -24100.00 -108900.00 410300.00 12900.00 -52300.00 218500.00 -50200.00 B -7300.00 -130200.00 -65600.00 -57800.00 -7500.00 9825200.00 10400.00 900.00 -95100.00 255100.00 -9800.00 C -97400.00 159100.00 364500.00 32200.00 -96500.00 -49200.00 19000.00 -50200.00 849500.00 13515200.00 189400.00 3170400.00 D 301400.00 -200.00 19200.00 48200.00 128000.00 78500.00 -81200.00 289400.00 37500.00 21668800.00 64400.00 444400.00 E 351400.00 -41600.00 233000.00 74300.00 9900.00 195500.00 3013100.00 5900.00 -37600.00 60400.00 467600.00 -27700.00 F 410100.00 -35100.00 43600.00 327700.00 106900.00 918000.00 112000.00 84400.00 -7900.00 36900.00 -16400.00 -43300.00 G 11200.00 5700.00 -152500.00 -112300.00 54100.00 851300.00 -61400.00 -65200.00 -64500.00 -90000.00 -42500.00 -3100.00 H 136200.00 -152900.00 -89600.00 -133100.00 -92700.00 -18900.00 19800.00 4900.00 -3900.00 -124900.00 -63300.00 227300.00 Load time: 10/24/2008 10:50:37 AM Read time: 10/24/2008 10:50:37 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: DNATech Fluorescence Int Plate ID: #1720 Comment: new comment Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 32.3 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f1 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 4 A/D converter gain: x1 Integrating gain: x1 Max cps: 224331 cps Min counts: 611 counts Well list: r0(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a1,a7,b1) = (f1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a1,a7,b1)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 863500.00 719500.00 714500.00 704000.00 740200.00 655400.00 683600.00 1174600.00 777200.00 712000.00 982800.00 714100.00 B 745800.00 757000.00 634100.00 698700.00 706500.00 756800.00 10589500.00 774700.00 765200.00 669200.00 1019400.00 754500.00 C 666900.00 923400.00 1128800.00 796500.00 667800.00 715100.00 783300.00 714100.00 1613800.00 14279500.00 953700.00 3934700.00 D 1065700.00 764100.00 783500.00 812500.00 892300.00 842800.00 683100.00 1053700.00 801800.00 22433100.00 828700.00 1208700.00 E 1115700.00 722700.00 997300.00 838600.00 774200.00 959800.00 3777400.00 770200.00 726700.00 824700.00 1231900.00 736600.00 F 1174400.00 729200.00 807900.00 1092000.00 871200.00 1682300.00 876300.00 848700.00 756400.00 801200.00 747900.00 721000.00 G 775500.00 770000.00 611800.00 652000.00 818400.00 1615600.00 702900.00 699100.00 699800.00 674300.00 721800.00 761200.00 H 900500.00 611400.00 674700.00 631200.00 671600.00 745400.00 784100.00 769200.00 760400.00 639400.00 701000.00 991600.00 Well list: r1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) = (f1(a2:a6,a8:a12,b2:b12,c1:c11,c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) - avg(f1(a1,a7,b1))) Data: BACKGROUND SUBTRACTED DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A -44800.00 -49800.00 -60300.00 -24100.00 -108900.00 410300.00 12900.00 -52300.00 218500.00 -50200.00 B -7300.00 -130200.00 -65600.00 -57800.00 -7500.00 9825200.00 10400.00 900.00 -95100.00 255100.00 -9800.00 C -97400.00 159100.00 364500.00 32200.00 -96500.00 -49200.00 19000.00 -50200.00 849500.00 13515200.00 189400.00 3170400.00 D 301400.00 -200.00 19200.00 48200.00 128000.00 78500.00 -81200.00 289400.00 37500.00 21668800.00 64400.00 444400.00 E 351400.00 -41600.00 233000.00 74300.00 9900.00 195500.00 3013100.00 5900.00 -37600.00 60400.00 467600.00 -27700.00 F 410100.00 -35100.00 43600.00 327700.00 106900.00 918000.00 112000.00 84400.00 -7900.00 36900.00 -16400.00 -43300.00 G 11200.00 5700.00 -152500.00 -112300.00 54100.00 851300.00 -61400.00 -65200.00 -64500.00 -90000.00 -42500.00 -3100.00 H 136200.00 -152900.00 -89600.00 -133100.00 -92700.00 -18900.00 19800.00 4900.00 -3900.00 -124900.00 -63300.00 227300.00 Load time: 10/24/2008 02:15:14 PM Read time: 10/24/2008 02:15:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: DNATech Fluorescence Int Plate ID: #1723 Comment: new comment Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 32.1 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f1 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 4 A/D converter gain: x1 Integrating gain: x1 Max cps: 175773136 cps Min counts: 1839 counts Well list: r0(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a6,a12,b6) = (f1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a6,a12,b6)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17577313600.00 157399100.00 632834900.00 178182200.00 91115100.00 4068200.00 11798470400.00 8879261600.00 17224300.00 189805100.00 27032000.00 1839900.00 B 13301750400.00 24542100.00 2026561000.00 23288100.00 98362700.00 5204000.00 4206536800.00 4872918800.00 4978545600.00 2939550600.00 7118085600.00 3573810800.00 C 452119900.00 3761084400.00 7919893600.00 5103931200.00 422821900.00 7361439200.00 4372022000.00 1653144700.00 5349617200.00 2334115000.00 731765800.00 4758499200.00 D 6890306400.00 4758499200.00 4372022000.00 9236336000.00 3462465600.00 9261165600.00 4468422400.00 960187700.00 5780458800.00 5219929600.00 3065083000.00 526138300.00 E 5939919600.00 7901728000.00 9261165600.00 4557081200.00 10162694400.00 6876553600.00 2385840200.00 4456861200.00 7622020800.00 8527607200.00 540585800.00 3696516400.00 F 2789598200.00 6904115200.00 6890306400.00 211449900.00 1279774700.00 8527607200.00 2617897800.00 6676654000.00 6917978400.00 1628144400.00 5939919600.00 3761084400.00 G 378921400.00 4866036000.00 3634128000.00 270505100.00 1169037500.00 5780458800.00 347188700.00 6475852400.00 4866036000.00 2980236400.00 44451300.00 4462634000.00 H 3978237200.00 331137400.00 5485913200.00 7622020800.00 6676654000.00 9261165600.00 7132823200.00 3250144800.00 4992976000.00 5503440000.00 3065083000.00 4758499200.00 Well list: r1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) = (f1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) - avg(f1(a6,a12,b6))) Data: BACKGROUND SUBTRACTED DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17573609566.67 153695066.67 629130866.67 174478166.67 87411066.67 11794766366.67 8875557566.67 13520266.67 186101066.67 23327966.67 B 13298046366.67 20838066.67 2022856966.67 19584066.67 94658666.67 4202832766.67 4869214766.67 4974841566.67 2935846566.67 7114381566.67 3570106766.67 C 448415866.67 3757380366.67 7916189566.67 5100227166.67 419117866.67 7357735166.67 4368317966.67 1649440666.67 5345913166.67 2330410966.67 728061766.67 4754795166.67 D 6886602366.67 4754795166.67 4368317966.67 9232631966.67 3458761566.67 9257461566.67 4464718366.67 956483666.67 5776754766.67 5216225566.67 3061378966.67 522434266.67 E 5936215566.67 7898023966.67 9257461566.67 4553377166.67 10158990366.67 6872849566.67 2382136166.67 4453157166.67 7618316766.67 8523903166.67 536881766.67 3692812366.67 F 2785894166.67 6900411166.67 6886602366.67 207745866.67 1276070666.67 8523903166.67 2614193766.67 6672949966.67 6914274366.67 1624440366.67 5936215566.67 3757380366.67 G 375217366.67 4862331966.67 3630423966.67 266801066.67 1165333466.67 5776754766.67 343484666.67 6472148366.67 4862331966.67 2976532366.67 40747266.67 4458929966.67 H 3974533166.67 327433366.67 5482209166.67 7618316766.67 6672949966.67 9257461566.67 7129119166.67 3246440766.67 4989271966.67 5499735966.67 3061378966.67 4754795166.67