##BLOCKS= 5 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 17906257.5 14622968.5 9770207.5 6107704.5 4063748.5 2555639.5 1787809.5 1141930.5 836475.5 536397.5 355748.5 -1182.5 18070275.5 13783169.5 9258904.5 6134417.5 4057447.5 2806058.5 1730048.5 1161877.5 862913.5 583596.5 351442.5 1182.5 -13058.5 -13007.5 -13028.5 -12990.5 -13010.5 -12908.5 -4904.5 -13000.5 -13041.5 -13054.5 -12909.5 -12998.5 -12919.5 -12949.5 -12784.5 -13071.5 -12997.5 -12806.5 -12964.5 -12902.5 -12930.5 -12968.5 -12902.5 -13065.5 -13003.5 -12977.5 -12912.5 -12955.5 -12884.5 -12910.5 -12963.5 -12930.5 -12885.5 -13027.5 -12951.5 -12895.5 -13007.5 -13014.5 -13017.5 -12842.5 -12968.5 -10321.5 -12907.5 -12945.5 -12983.5 -13036.5 -13018.5 -12949.5 -12972.5 -4131.5 -12942.5 -12962.5 -12639.5 -12940.5 -12963.5 -12993.5 -12902.5 -12958.5 -12999.5 -13012.5 -13059.5 -13069.5 -12955.5 -12995.5 -13000.5 -12919.5 -12917.5 -12798.5 -12914.5 -12984.5 -12979.5 -12997.5 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 2279862 5347819 11048 5278887 9666 8773 9183 8558 3730741 3173923 1929821 8125 2939006 10075 456359 3481874 4774943 1862141 9423 879524 210499 1969628 2624347 2814869 2420862 881140 5601868 3405714 683702 3549548 2911973 1736903 148093 1033113 3419428 1902794 2233302 3254764 2863119 4509086 2960680 3027272 3068203 4118723 1058322 669859 2508673 4404363 353096 3561357 919027 2412153 156918 2802690 3797762 2484832 2365101 11679060 834736 3968154 3234619 3497049 5166199 5258057 2971733 715881 4629876 860148 4114287 633955 3590586 1660911 1357768 2945630 3253714 2581705 6498993 695608 4511748 2728059 2192568 1979520 971255 2764704 1686387 3553795 5222834 1765985 1758012 3494281 4704078 3830004 5695262 10741 1827828 2351936 ~End Plate: Plate3 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 12105 13666 20441 10736 10334 10848 11939 12619 10438447 11167 13284 11682 15825 241417 12715 5371340 7327110 3631381 11172 1850887 510302 38703 10683 20837 11178 12591 8580585 11427 1109992 17199 5452260 13096 327376 11912 10581 11255 13846 16157 4614079 23005 5127407 17809 18868 28338 11876 13324 20622 15738 92650 12224 12220 14000 12952 11491 8137691 12291 11141 11695 10796 11588 11505 25785 13311 11734 11896 12927 10002012 12075 13115 53238 10796 10654 52585 14222 13039 17887 13569 12468 9801977 19924 30875 11268 14854 11434 12680 14240 12796 12440 28641 21671 31730 12296 71306 10379 9907 11292 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 100.000 93.020 A1 17906257.500 17988266.500 115978.240 0.6 93.886 B1 18070275.500 02 66.667 75.696 A2 14622968.500 14203069.000 593827.568 4.2 71.265 B2 13783169.500 03 44.444 50.092 A3 9770207.500 9514556.000 361545.819 3.8 47.394 B3 9258904.500 04 29.630 30.767 A4 6107704.500 6121061.000 18888.943 0.3 30.908 B4 6134417.500 05 19.753 19.982 A5 4063748.500 4060598.000 4455.480 0.1 19.949 B5 4057447.500 06 13.169 12.025 A6 2555639.500 2680849.000 177072.973 6.6 13.346 B6 2806058.500 07 8.779 7.974 A7 1787809.500 1758929.000 40843.195 2.3 7.669 B7 1730048.500 08 5.853 4.566 A8 1141930.500 1151904.000 14104.659 1.2 4.671 B8 1161877.500 09 3.902 2.954 A9 836475.500 849694.500 18694.489 2.2 3.094 B9 862913.500 10 2.601 1.371 A10 536397.500 559997.000 33374.733 6.0 1.620 B10 583596.500 11 1.734 0.418 A11 355748.500 353595.500 3044.802 0.9 0.395 B11 351442.500 Group Summaries MinStd Smallest standard value: 353595.500 Min(MeanValue) MaxStd Largest standard value: 17988266.500 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 2279862.000 10.570 10.570 0.000 0.0 02 B1 2939006.000 14.048 14.048 0.000 0.0 03 C1 2420862.000 11.314 11.314 0.000 0.0 04 D1 2233302.000 10.324 10.324 0.000 0.0 05 E1 353096.000 R 0.404 0.404 0.000 0.0 06 F1 3234619.000 15.608 15.608 0.000 0.0 07 G1 1357768.000 5.705 5.705 0.000 0.0 08 H1 1686387.000 7.439 7.439 0.000 0.0 09 A2 5347819.000 26.758 26.758 0.000 0.0 097 A1 12105.000 R -1.395 -1.395 0.000 0.0 098 B1 15825.000 R -1.376 -1.376 0.000 0.0 099 C1 11178.000 R -1.400 -1.400 0.000 0.0 10 B2 10075.000 R -1.406 -1.406 0.000 0.0 100 D1 13846.000 R -1.386 -1.386 0.000 0.0 101 E1 92650.000 R -0.970 -0.970 0.000 0.0 102 F1 11505.000 R -1.399 -1.399 0.000 0.0 103 G1 52585.000 R -1.182 -1.182 0.000 0.0 104 H1 12680.000 R -1.392 -1.392 0.000 0.0 105 A2 13666.000 R -1.387 -1.387 0.000 0.0 106 B2 241417.000 R -0.185 -0.185 0.000 0.0 107 C2 12591.000 R -1.393 -1.393 0.000 0.0 108 D2 16157.000 R -1.374 -1.374 0.000 0.0 109 E2 12224.000 R -1.395 -1.395 0.000 0.0 11 C2 881140.000 3.190 3.190 0.000 0.0 110 F2 25785.000 R -1.323 -1.323 0.000 0.0 111 G2 14222.000 R -1.384 -1.384 0.000 0.0 112 H2 14240.000 R -1.384 -1.384 0.000 0.0 113 A3 20441.000 R -1.351 -1.351 0.000 0.0 114 B3 12715.000 R -1.392 -1.392 0.000 0.0 115 C3 8580585.000 43.815 43.815 0.000 0.0 116 D3 4614079.000 22.886 22.886 0.000 0.0 117 E3 12220.000 R -1.395 -1.395 0.000 0.0 118 F3 13311.000 R -1.389 -1.389 0.000 0.0 119 G3 13039.000 R -1.390 -1.390 0.000 0.0 12 D2 3254764.000 15.714 15.714 0.000 0.0 120 H3 12796.000 R -1.392 -1.392 0.000 0.0 121 A4 10736.000 R -1.403 -1.403 0.000 0.0 122 B4 5371340.000 26.882 26.882 0.000 0.0 123 C4 11427.000 R -1.399 -1.399 0.000 0.0 124 D4 23005.000 R -1.338 -1.338 0.000 0.0 125 E4 14000.000 R -1.385 -1.385 0.000 0.0 126 F4 11734.000 R -1.397 -1.397 0.000 0.0 127 G4 17887.000 R -1.365 -1.365 0.000 0.0 128 H4 12440.000 R -1.394 -1.394 0.000 0.0 129 A5 10334.000 R -1.405 -1.405 0.000 0.0 13 E2 3561357.000 17.332 17.332 0.000 0.0 130 B5 7327110.000 37.201 37.201 0.000 0.0 131 C5 1109992.000 4.397 4.397 0.000 0.0 132 D5 5127407.000 25.595 25.595 0.000 0.0 133 E5 12952.000 R -1.391 -1.391 0.000 0.0 134 F5 11896.000 R -1.396 -1.396 0.000 0.0 135 G5 13569.000 R -1.388 -1.388 0.000 0.0 136 H5 28641.000 R -1.308 -1.308 0.000 0.0 137 A6 10848.000 R -1.402 -1.402 0.000 0.0 138 B6 3631381.000 17.701 17.701 0.000 0.0 139 C6 17199.000 R -1.368 -1.368 0.000 0.0 14 F2 3497049.000 16.992 16.992 0.000 0.0 140 D6 17809.000 R -1.365 -1.365 0.000 0.0 141 E6 11491.000 R -1.399 -1.399 0.000 0.0 142 F6 12927.000 R -1.391 -1.391 0.000 0.0 143 G6 12468.000 R -1.393 -1.393 0.000 0.0 144 H6 21671.000 R -1.345 -1.345 0.000 0.0 145 A7 11939.000 R -1.396 -1.396 0.000 0.0 146 B7 11172.000 R -1.400 -1.400 0.000 0.0 147 C7 5452260.000 27.309 27.309 0.000 0.0 148 D7 18868.000 R -1.360 -1.360 0.000 0.0 149 E7 8137691.000 41.478 41.478 0.000 0.0 15 G2 2945630.000 14.083 14.083 0.000 0.0 150 F7 10002012.000 51.315 51.315 0.000 0.0 151 G7 9801977.000 50.259 50.259 0.000 0.0 152 H7 31730.000 R -1.292 -1.292 0.000 0.0 153 A8 12619.000 R -1.393 -1.393 0.000 0.0 154 B8 1850887.000 8.307 8.307 0.000 0.0 155 C8 13096.000 R -1.390 -1.390 0.000 0.0 156 D8 28338.000 R -1.310 -1.310 0.000 0.0 157 E8 12291.000 R -1.394 -1.394 0.000 0.0 158 F8 12075.000 R -1.396 -1.396 0.000 0.0 159 G8 19924.000 R -1.354 -1.354 0.000 0.0 16 H2 3553795.000 17.292 17.292 0.000 0.0 160 H8 12296.000 R -1.394 -1.394 0.000 0.0 161 A9 10438447.000 53.617 53.617 0.000 0.0 162 B9 510302.000 1.233 1.233 0.000 0.0 163 C9 327376.000 R 0.268 0.268 0.000 0.0 164 D9 11876.000 R -1.397 -1.397 0.000 0.0 165 E9 11141.000 R -1.400 -1.400 0.000 0.0 166 F9 13115.000 R -1.390 -1.390 0.000 0.0 167 G9 30875.000 R -1.296 -1.296 0.000 0.0 168 H9 71306.000 R -1.083 -1.083 0.000 0.0 169 A10 11167.000 R -1.400 -1.400 0.000 0.0 17 A3 11048.000 R -1.401 -1.401 0.000 0.0 170 B10 38703.000 R -1.255 -1.255 0.000 0.0 171 C10 11912.000 R -1.396 -1.396 0.000 0.0 172 D10 13324.000 R -1.389 -1.389 0.000 0.0 173 E10 11695.000 R -1.398 -1.398 0.000 0.0 174 F10 53238.000 R -1.178 -1.178 0.000 0.0 175 G10 11268.000 R -1.400 -1.400 0.000 0.0 176 H10 10379.000 R -1.404 -1.404 0.000 0.0 177 A11 13284.000 R -1.389 -1.389 0.000 0.0 178 B11 10683.000 R -1.403 -1.403 0.000 0.0 179 C11 10581.000 R -1.403 -1.403 0.000 0.0 18 B3 456359.000 0.949 0.949 0.000 0.0 180 D11 20622.000 R -1.350 -1.350 0.000 0.0 181 E11 10796.000 R -1.402 -1.402 0.000 0.0 182 F11 10796.000 R -1.402 -1.402 0.000 0.0 183 G11 14854.000 R -1.381 -1.381 0.000 0.0 184 H11 9907.000 R -1.407 -1.407 0.000 0.0 185 A12 11682.000 R -1.398 -1.398 0.000 0.0 186 B12 20837.000 R -1.349 -1.349 0.000 0.0 187 C12 11255.000 R -1.400 -1.400 0.000 0.0 188 D12 15738.000 R -1.376 -1.376 0.000 0.0 189 E12 11588.000 R -1.398 -1.398 0.000 0.0 19 C3 5601868.000 28.098 28.098 0.000 0.0 190 F12 10654.000 R -1.403 -1.403 0.000 0.0 191 G12 11434.000 R -1.399 -1.399 0.000 0.0 192 H12 11292.000 R -1.400 -1.400 0.000 0.0 20 D3 2863119.000 13.648 13.648 0.000 0.0 21 E3 919027.000 3.390 3.390 0.000 0.0 22 F3 5166199.000 25.799 25.799 0.000 0.0 23 G3 3253714.000 15.708 15.708 0.000 0.0 24 H3 5222834.000 26.098 26.098 0.000 0.0 25 A4 5278887.000 26.394 26.394 0.000 0.0 26 B4 3481874.000 16.912 16.912 0.000 0.0 27 C4 3405714.000 16.510 16.510 0.000 0.0 28 D4 4509086.000 22.332 22.332 0.000 0.0 29 E4 2412153.000 11.268 11.268 0.000 0.0 30 F4 5258057.000 26.284 26.284 0.000 0.0 31 G4 2581705.000 12.163 12.163 0.000 0.0 32 H4 1765985.000 7.859 7.859 0.000 0.0 33 A5 9666.000 R -1.408 -1.408 0.000 0.0 34 B5 4774943.000 23.735 23.735 0.000 0.0 35 C5 683702.000 2.148 2.148 0.000 0.0 36 D5 2960680.000 14.162 14.162 0.000 0.0 37 E5 156918.000 R -0.631 -0.631 0.000 0.0 38 F5 2971733.000 14.221 14.221 0.000 0.0 39 G5 6498993.000 32.832 32.832 0.000 0.0 40 H5 1758012.000 7.817 7.817 0.000 0.0 41 A6 8773.000 R -1.413 -1.413 0.000 0.0 42 B6 1862141.000 8.366 8.366 0.000 0.0 43 C6 3549548.000 17.269 17.269 0.000 0.0 44 D6 3027272.000 14.514 14.514 0.000 0.0 45 E6 2802690.000 13.329 13.329 0.000 0.0 46 F6 715881.000 2.318 2.318 0.000 0.0 47 G6 695608.000 2.211 2.211 0.000 0.0 48 H6 3494281.000 16.978 16.978 0.000 0.0 49 A7 9183.000 R -1.411 -1.411 0.000 0.0 50 B7 9423.000 R -1.410 -1.410 0.000 0.0 51 C7 2911973.000 13.905 13.905 0.000 0.0 52 D7 3068203.000 14.730 14.730 0.000 0.0 53 E7 3797762.000 18.579 18.579 0.000 0.0 54 F7 4629876.000 22.970 22.970 0.000 0.0 55 G7 4511748.000 22.346 22.346 0.000 0.0 56 H7 4704078.000 23.361 23.361 0.000 0.0 57 A8 8558.000 R -1.414 -1.414 0.000 0.0 58 B8 879524.000 3.181 3.181 0.000 0.0 59 C8 1736903.000 7.705 7.705 0.000 0.0 60 D8 4118723.000 20.273 20.273 0.000 0.0 61 E8 2484832.000 11.652 11.652 0.000 0.0 62 F8 860148.000 3.079 3.079 0.000 0.0 63 G8 2728059.000 12.935 12.935 0.000 0.0 64 H8 3830004.000 18.749 18.749 0.000 0.0 65 A9 3730741.000 18.225 18.225 0.000 0.0 66 B9 210499.000 R -0.349 -0.349 0.000 0.0 67 C9 148093.000 R -0.678 -0.678 0.000 0.0 68 D9 1058322.000 4.125 4.125 0.000 0.0 69 E9 2365101.000 11.020 11.020 0.000 0.0 70 F9 4114287.000 20.249 20.249 0.000 0.0 71 G9 2192568.000 10.109 10.109 0.000 0.0 72 H9 5695262.000 28.591 28.591 0.000 0.0 73 A10 3173923.000 15.287 15.287 0.000 0.0 74 B10 1969628.000 8.933 8.933 0.000 0.0 75 C10 1033113.000 3.992 3.992 0.000 0.0 76 D10 669859.000 2.075 2.075 0.000 0.0 77 E10 11679060.000 60.163 60.163 0.000 0.0 78 F10 633955.000 1.886 1.886 0.000 0.0 79 G10 1979520.000 8.985 8.985 0.000 0.0 80 H10 10741.000 R -1.403 -1.403 0.000 0.0 81 A11 1929821.000 8.723 8.723 0.000 0.0 82 B11 2624347.000 12.388 12.388 0.000 0.0 83 C11 3419428.000 16.583 16.583 0.000 0.0 84 D11 2508673.000 11.777 11.777 0.000 0.0 85 E11 834736.000 2.945 2.945 0.000 0.0 86 F11 3590586.000 17.486 17.486 0.000 0.0 87 G11 971255.000 3.665 3.665 0.000 0.0 88 H11 1827828.000 8.185 8.185 0.000 0.0 89 A12 8125.000 R -1.416 -1.416 0.000 0.0 90 B12 2814869.000 13.393 13.393 0.000 0.0 91 C12 1902794.000 8.581 8.581 0.000 0.0 92 D12 4404363.000 21.780 21.780 0.000 0.0 93 E12 3968154.000 19.478 19.478 0.000 0.0 94 F12 1660911.000 7.304 7.304 0.000 0.0 95 G12 2764704.000 13.128 13.128 0.000 0.0 96 H12 2351936.000 10.950 10.950 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: HiISeq1396_libraries_AliquotedByOpentrons; Date Last Saved: 4/9/2019 3:01:14 PM