##BLOCKS= 4 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 2393695 1986504 1481166 2036950 1885818 4510472 5777186 1960073 4957654 4004068 -15902 19796215 2144658 2929971 1950099 1940618 3920316 2990969 4994592 1657495 5019595 4570217 -15907 9138147 1285693 1707837 1593973 1595532 2653938 2484178 2455346 4452634 3811701 4004525 -15937 4596165 1249597 5078260 2230470 1455908 3781807 2590035 2629251 2795909 4012376 3959882 -15919 2143019 1225707 3561066 1078191 1601390 2338931 5471389 3971177 3791533 4713264 11846018 -15821 767870 544318 1603026 2787690 1871141 2930863 3007 3739806 1776523 3499027 3235 -15845 343126 918157 2069593 2268343 644 2003128 2236269 4916038 4119437 5673519 8700462 -14533 159540 1588577 1473800 2200517 517 3595826 3157475 4459569 6321558 8479424 5070972 -15999 0 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 50.000 50.574 A12 19796215.000 19796215.000 0.000 0.0 02 25.000 23.853 B12 9138147.000 9138147.000 0.000 0.0 03 12.500 12.465 C12 4596165.000 4596165.000 0.000 0.0 04 6.250 6.315 D12 2143019.000 2143019.000 0.000 0.0 05 3.125 2.867 E12 767870.000 767870.000 0.000 0.0 06 1.563 1.802 F12 343126.000 343126.000 0.000 0.0 07 0.781 1.342 G12 159540.000 159540.000 0.000 0.0 Group Summaries MinStd Smallest standard value: 159540.000 Min(MeanValue) MaxStd Largest standard value: 19796215.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 2393695.000 6.943 6.943 0.000 0.0 02 A2 1986504.000 5.923 5.923 0.000 0.0 03 A3 1481166.000 4.656 4.656 0.000 0.0 04 A4 2036950.000 6.049 6.049 0.000 0.0 05 A5 1885818.000 5.670 5.670 0.000 0.0 06 A6 4510472.000 12.250 12.250 0.000 0.0 07 A7 5777186.000 15.426 15.426 0.000 0.0 08 A8 1960073.000 5.856 5.856 0.000 0.0 09 A9 4957654.000 13.372 13.372 0.000 0.0 10 A10 4004068.000 10.981 10.981 0.000 0.0 11 A11 -15902.000 R 0.902 0.902 0.000 0.0 12 B1 2144658.000 6.319 6.319 0.000 0.0 13 B2 2929971.000 8.288 8.288 0.000 0.0 14 B3 1950099.000 5.831 5.831 0.000 0.0 15 B4 1940618.000 5.808 5.808 0.000 0.0 16 B5 3920316.000 10.771 10.771 0.000 0.0 17 B6 2990969.000 8.441 8.441 0.000 0.0 18 B7 4994592.000 13.464 13.464 0.000 0.0 19 B8 1657495.000 5.098 5.098 0.000 0.0 20 B9 5019595.000 13.527 13.527 0.000 0.0 21 B10 4570217.000 12.400 12.400 0.000 0.0 22 B11 -15907.000 R 0.902 0.902 0.000 0.0 23 C1 1285693.000 4.166 4.166 0.000 0.0 24 C2 1707837.000 5.224 5.224 0.000 0.0 25 C3 1593973.000 4.938 4.938 0.000 0.0 26 C4 1595532.000 4.942 4.942 0.000 0.0 27 C5 2653938.000 7.596 7.596 0.000 0.0 28 C6 2484178.000 7.170 7.170 0.000 0.0 29 C7 2455346.000 7.098 7.098 0.000 0.0 30 C8 4452634.000 12.105 12.105 0.000 0.0 31 C9 3811701.000 10.499 10.499 0.000 0.0 32 C10 4004525.000 10.982 10.982 0.000 0.0 33 C11 -15937.000 R 0.902 0.902 0.000 0.0 34 D1 1249597.000 4.075 4.075 0.000 0.0 35 D2 5078260.000 13.674 13.674 0.000 0.0 36 D3 2230470.000 6.534 6.534 0.000 0.0 37 D4 1455908.000 4.592 4.592 0.000 0.0 38 D5 3781807.000 10.424 10.424 0.000 0.0 39 D6 2590035.000 7.436 7.436 0.000 0.0 40 D7 2629251.000 7.534 7.534 0.000 0.0 41 D8 2795909.000 7.952 7.952 0.000 0.0 42 D9 4012376.000 11.002 11.002 0.000 0.0 43 D10 3959882.000 10.870 10.870 0.000 0.0 44 D11 -15919.000 R 0.902 0.902 0.000 0.0 45 E1 1225707.000 4.015 4.015 0.000 0.0 46 E2 3561066.000 9.870 9.870 0.000 0.0 47 E3 1078191.000 3.645 3.645 0.000 0.0 48 E4 1601390.000 4.957 4.957 0.000 0.0 49 E5 2338931.000 6.806 6.806 0.000 0.0 50 E6 5471389.000 14.660 14.660 0.000 0.0 51 E7 3971177.000 10.898 10.898 0.000 0.0 52 E8 3791533.000 10.448 10.448 0.000 0.0 53 E9 4713264.000 12.759 12.759 0.000 0.0 54 E10 11846018.000 30.642 30.642 0.000 0.0 55 E11 -15821.000 R 0.902 0.902 0.000 0.0 56 F1 544318.000 2.307 2.307 0.000 0.0 57 F2 1603026.000 4.961 4.961 0.000 0.0 58 F3 2787690.000 7.931 7.931 0.000 0.0 59 F4 1871141.000 5.633 5.633 0.000 0.0 60 F5 2930863.000 8.290 8.290 0.000 0.0 61 F6 3007.000 R 0.950 0.950 0.000 0.0 62 F7 3739806.000 10.318 10.318 0.000 0.0 63 F8 1776523.000 5.396 5.396 0.000 0.0 64 F9 3499027.000 9.715 9.715 0.000 0.0 65 F10 3235.000 R 0.950 0.950 0.000 0.0 66 F11 -15845.000 R 0.902 0.902 0.000 0.0 67 G1 918157.000 3.244 3.244 0.000 0.0 68 G2 2069593.000 6.131 6.131 0.000 0.0 69 G3 2268343.000 6.629 6.629 0.000 0.0 70 G4 644.000 R 0.944 0.944 0.000 0.0 71 G5 2003128.000 5.964 5.964 0.000 0.0 72 G6 2236269.000 6.549 6.549 0.000 0.0 73 G7 4916038.000 13.267 13.267 0.000 0.0 74 G8 4119437.000 11.270 11.270 0.000 0.0 75 G9 5673519.000 15.166 15.166 0.000 0.0 76 G10 8700462.000 22.755 22.755 0.000 0.0 77 G11 -14533.000 R 0.906 0.906 0.000 0.0 78 H1 1588577.000 4.925 4.925 0.000 0.0 79 H2 1473800.000 4.637 4.637 0.000 0.0 80 H3 2200517.000 6.459 6.459 0.000 0.0 81 H4 517.000 R 0.943 0.943 0.000 0.0 82 H5 3595826.000 9.957 9.957 0.000 0.0 83 H6 3157475.000 8.858 8.858 0.000 0.0 84 H7 4459569.000 12.123 12.123 0.000 0.0 85 H8 6321558.000 16.791 16.791 0.000 0.0 86 H9 8479424.000 22.201 22.201 0.000 0.0 87 H10 5070972.000 13.656 13.656 0.000 0.0 88 H11 -15999.000 R 0.902 0.902 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: HiSeq268_Libraries_and_Oanh_48Samples_01-12-2016; Date Last Saved: 1/12/2016 3:05:03 PM