##BLOCKS= 4 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 44082864 32256434 30282824 30566732 25815406 42379988 30302872 27077324 37290148 15521602 25267172 30770370 35536580 31172258 25549868 27964768 20237852 30082218 28737640 29210030 12720482 24260458 34422292 37104460 36149256 32578298 2140652 28832472 22805952 41205976 30842644 11428032 33411138 22520862 30964606 27777138 34179324 34599288 27806236 31158408 18310194 36134584 14569419 24905092 19220400 4772120 18506292 29579704 32317206 30992972 29068092 25012738 20611076 39792904 20591920 12108422 2749060 2468207 28872684 10463633 25049636 32638718 34573780 18810030 21221212 21246806 31390858 25848416 5334335 7669931 24753822 26211338 29700246 30645710 29593750 25909090 23796088 25557172 34092120 24015768 21931990 11695229 29854350 26166136 25008888 39536692 28923652 25499538 28599858 46751544 27736962 27749826 24240112 6123016 33605792 32895938 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 28275809.5 36737141.5 30444513.5 40183241.5 40479405.5 -18626.5 -18693.5 -18690.5 -18576.5 -18712.5 -18648.5 -18637.5 19084417.5 34443417.5 9814396.5 19692377.5 19863427.5 -18611.5 -18613.5 -18655.5 -18552.5 -18497.5 -18698.5 -18679.5 24262713.5 46736981.5 15382819.5 10009166.5 9974073.5 -18662.5 -18529.5 -18543.5 -18397.5 -18532.5 -18571.5 -18541.5 34124629.5 17129569.5 -18302.5 5017503.5 5102941.5 -18652.5 -18696.5 -18622.5 -18602.5 -18577.5 -18569.5 -18624.5 30680447.5 6167149.5 -18229.5 1779640.5 1739166.5 -18593.5 -18477.5 -18544.5 -18563.5 -18555.5 -18604.5 -18641.5 17644519.5 42320425.5 -18328.5 486757.5 434933.5 -18520.5 -18528.5 -18523.5 -18605.5 -18613.5 -18646.5 -18478.5 28044047.5 3802551.5 -18090.5 232713.5 248928.5 -18599.5 -18481.5 -18627.5 -18619.5 -18616.5 -18580.5 -18616.5 18087609.5 10252851.5 -18223.5 -894.5 894.5 -18527.5 -18526.5 -18631.5 -18518.5 -18490.5 -18545.5 -18548.5 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 100.000 99.592 A4 40183241.500 40331323.500 209419.573 0.5 100.317 A5 40479405.500 02 50.000 49.416 B4 19692377.500 19777902.500 120950.615 0.6 49.835 B5 19863427.500 03 25.000 25.704 C4 10009166.500 9991620.000 24814.498 0.2 25.618 C5 9974073.500 04 12.500 13.481 D4 5017503.500 5060222.500 60413.789 1.2 13.690 D5 5102941.500 05 6.250 5.553 E4 1779640.500 1759403.500 28619.440 1.6 5.453 E5 1739166.500 06 3.125 2.387 F4 486757.500 460845.500 36645.102 8.0 2.260 F5 434933.500 07 1.563 1.765 G4 232713.500 240821.000 11465.736 4.8 1.804 G5 248928.500 Group Summaries MinStd Smallest standard value: 240821.000 Min(MeanValue) MaxStd Largest standard value: 40331323.500 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 44082864.000 R 109.141 109.141 0.000 0.0 02 A2 32256434.000 80.182 80.182 0.000 0.0 03 A3 30282824.000 75.349 75.349 0.000 0.0 04 A4 30566732.000 76.044 76.044 0.000 0.0 05 A5 25815406.000 64.409 64.409 0.000 0.0 06 A6 42379988.000 R 104.971 104.971 0.000 0.0 07 A7 30302872.000 75.398 75.398 0.000 0.0 08 A8 27077324.000 67.499 67.499 0.000 0.0 09 A9 37290148.000 92.508 92.508 0.000 0.0 097 A1 28275809.500 70.434 70.434 0.000 0.0 098 B1 19084417.500 47.927 47.927 0.000 0.0 099 C1 24262713.500 60.607 60.607 0.000 0.0 10 A10 15521602.000 39.203 39.203 0.000 0.0 100 D1 34124629.500 84.756 84.756 0.000 0.0 101 E1 30680447.500 76.322 76.322 0.000 0.0 102 F1 17644519.500 44.401 44.401 0.000 0.0 103 G1 28044047.500 69.867 69.867 0.000 0.0 104 H1 18087609.500 45.486 45.486 0.000 0.0 105 A2 36737141.500 91.154 91.154 0.000 0.0 106 B2 34443417.500 85.537 85.537 0.000 0.0 107 C2 46736981.500 R 115.640 115.640 0.000 0.0 108 D2 17129569.500 43.140 43.140 0.000 0.0 109 E2 6167149.500 16.296 16.296 0.000 0.0 11 A11 25267172.000 63.067 63.067 0.000 0.0 110 F2 42320425.500 R 104.825 104.825 0.000 0.0 111 G2 3802551.500 10.506 10.506 0.000 0.0 112 H2 10252851.500 26.301 26.301 0.000 0.0 113 A3 30444513.500 75.745 75.745 0.000 0.0 114 B3 9814396.500 25.227 25.227 0.000 0.0 115 C3 15382819.500 38.863 38.863 0.000 0.0 12 A12 30770370.000 76.543 76.543 0.000 0.0 13 B1 35536580.000 88.214 88.214 0.000 0.0 14 B2 31172258.000 77.527 77.527 0.000 0.0 15 B3 25549868.000 63.759 63.759 0.000 0.0 16 B4 27964768.000 69.672 69.672 0.000 0.0 17 B5 20237852.000 50.751 50.751 0.000 0.0 18 B6 30082218.000 74.857 74.857 0.000 0.0 19 B7 28737640.000 71.565 71.565 0.000 0.0 20 B8 29210030.000 72.722 72.722 0.000 0.0 21 B9 12720482.000 32.344 32.344 0.000 0.0 22 B10 24260458.000 60.602 60.602 0.000 0.0 23 B11 34422292.000 85.485 85.485 0.000 0.0 24 B12 37104460.000 92.053 92.053 0.000 0.0 25 C1 36149256.000 89.714 89.714 0.000 0.0 26 C2 32578298.000 80.970 80.970 0.000 0.0 27 C3 2140652.000 6.437 6.437 0.000 0.0 28 C4 28832472.000 71.797 71.797 0.000 0.0 29 C5 22805952.000 57.040 57.040 0.000 0.0 30 C6 41205976.000 R 102.096 102.096 0.000 0.0 31 C7 30842644.000 76.720 76.720 0.000 0.0 32 C8 11428032.000 29.179 29.179 0.000 0.0 33 C9 33411138.000 83.009 83.009 0.000 0.0 34 C10 22520862.000 56.342 56.342 0.000 0.0 35 C11 30964606.000 77.018 77.018 0.000 0.0 36 C12 27777138.000 69.213 69.213 0.000 0.0 37 D1 34179324.000 84.890 84.890 0.000 0.0 38 D2 34599288.000 85.919 85.919 0.000 0.0 39 D3 27806236.000 69.284 69.284 0.000 0.0 40 D4 31158408.000 77.493 77.493 0.000 0.0 41 D5 18310194.000 46.031 46.031 0.000 0.0 42 D6 36134584.000 89.678 89.678 0.000 0.0 43 D7 14569419.000 36.871 36.871 0.000 0.0 44 D8 24905092.000 62.180 62.180 0.000 0.0 45 D9 19220400.000 48.260 48.260 0.000 0.0 46 D10 4772120.000 12.880 12.880 0.000 0.0 47 D11 18506292.000 46.511 46.511 0.000 0.0 48 D12 29579704.000 73.627 73.627 0.000 0.0 49 E1 32317206.000 80.330 80.330 0.000 0.0 50 E2 30992972.000 77.088 77.088 0.000 0.0 51 E3 29068092.000 72.374 72.374 0.000 0.0 52 E4 25012738.000 62.444 62.444 0.000 0.0 53 E5 20611076.000 51.665 51.665 0.000 0.0 54 E6 39792904.000 98.636 98.636 0.000 0.0 55 E7 20591920.000 51.618 51.618 0.000 0.0 56 E8 12108422.000 30.845 30.845 0.000 0.0 57 E9 2749060.000 7.926 7.926 0.000 0.0 58 E10 2468207.000 7.239 7.239 0.000 0.0 59 E11 28872684.000 71.896 71.896 0.000 0.0 60 E12 10463633.000 26.817 26.817 0.000 0.0 61 F1 25049636.000 62.534 62.534 0.000 0.0 62 F2 32638718.000 81.118 81.118 0.000 0.0 63 F3 34573780.000 85.856 85.856 0.000 0.0 64 F4 18810030.000 47.255 47.255 0.000 0.0 65 F5 21221212.000 53.159 53.159 0.000 0.0 66 F6 21246806.000 53.222 53.222 0.000 0.0 67 F7 31390858.000 78.062 78.062 0.000 0.0 68 F8 25848416.000 64.490 64.490 0.000 0.0 69 F9 5334335.000 14.257 14.257 0.000 0.0 70 F10 7669931.000 19.976 19.976 0.000 0.0 71 F11 24753822.000 61.810 61.810 0.000 0.0 72 F12 26211338.000 65.379 65.379 0.000 0.0 73 G1 29700246.000 73.922 73.922 0.000 0.0 74 G2 30645710.000 76.237 76.237 0.000 0.0 75 G3 29593750.000 73.661 73.661 0.000 0.0 76 G4 25909090.000 64.639 64.639 0.000 0.0 77 G5 23796088.000 59.465 59.465 0.000 0.0 78 G6 25557172.000 63.777 63.777 0.000 0.0 79 G7 34092120.000 84.677 84.677 0.000 0.0 80 G8 24015768.000 60.002 60.002 0.000 0.0 81 G9 21931990.000 54.900 54.900 0.000 0.0 82 G10 11695229.000 29.833 29.833 0.000 0.0 83 G11 29854350.000 74.300 74.300 0.000 0.0 84 G12 26166136.000 65.268 65.268 0.000 0.0 85 H1 25008888.000 62.434 62.434 0.000 0.0 86 H2 39536692.000 98.009 98.009 0.000 0.0 87 H3 28923652.000 72.021 72.021 0.000 0.0 88 H4 25499538.000 63.636 63.636 0.000 0.0 89 H5 28599858.000 71.228 71.228 0.000 0.0 90 H6 46751544.000 R 115.676 115.676 0.000 0.0 91 H7 27736962.000 69.115 69.115 0.000 0.0 92 H8 27749826.000 69.146 69.146 0.000 0.0 93 H9 24240112.000 60.552 60.552 0.000 0.0 94 H10 6123016.000 16.188 16.188 0.000 0.0 95 H11 33605792.000 83.486 83.486 0.000 0.0 96 H12 32895938.000 81.747 81.747 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: HiSeq466_Libraries_05-02-2016; Date Last Saved: 5/2/2016 2:06:54 PM