##BLOCKS= 4 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 50668142 50680322 -134694 -134719 -134755 -134772 -134860 -134753 -134778 -134849 -134786 -134822 33323748 33989594 -134823 -134878 -134764 -134712 -134756 -134665 -134737 -134689 -134656 -134711 15180729 14296249 -134704 -134754 -134738 -134705 -134743 -134674 -134788 -134868 -134733 -134785 5880957 6697947 -134731 -134695 -134667 -134736 -134766 -134667 -134631 -134816 -134827 -134686 1890936 2118276 -134690 -134825 -134886 -134803 -134674 -134790 -134817 -134713 -134749 -134771 680878 774487 -134742 -134739 -134835 -134780 -134791 -134711 -134829 -134650 -134763 -134724 228407 266483 -134717 -134778 -134753 -134630 -134696 -134765 -134658 -134713 -134826 -134801 0 7412 -134728 -134703 -134706 -134769 -134706 -134651 -134758 -134763 -134840 -134741 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 12814562 29156904 6637623 31525540 33941872 12063335 13750599 30868270 20088456 16035970 16586703 14733480 35309548 23882312 13409260 8145976 29247336 6016573 17703368 19692534 16217575 12367023 3711687 4801309 15560951 20661004 31597050 18248066 10799317 10132179 7546503 17883294 17053766 14803003 3535895 10549110 28656816 22237428 15346543 16155585 13365123 29742706 11347259 23582564 17440940 26892144 4759886 9329132 32044182 33567452 21942298 18471868 15211899 10640007 20788720 20248672 17998288 5806123 7170897 3481822 30650954 26831980 12822788 18642832 14148807 17756518 12506397 24220886 21868776 8678100 2984495 26398446 14934855 21420318 16108749 17497778 26109182 12731149 19438714 20347258 16530366 19228064 2764438 7966640 15428695 20037574 15451481 23591998 24368862 20395820 17400090 24170890 18874742 7120646 17285102 24686224 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 500.000 469.724 A1 50668142.000 50668142.000 0.000 0.0 02 250.000 308.391 B1 33323748.000 33323748.000 0.000 0.0 03 125.000 139.630 C1 15180729.000 15180729.000 0.000 0.0 04 62.500 53.126 D1 5880957.000 5880957.000 0.000 0.0 05 31.250 16.012 E1 1890936.000 1890936.000 0.000 0.0 06 15.625 4.756 F1 680878.000 680878.000 0.000 0.0 07 7.813 0.548 G1 228407.000 228407.000 0.000 0.0 Group Summaries MinStd Smallest standard value: 228407.000 Min(MeanValue) MaxStd Largest standard value: 50668142.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 12814562.000 117.621 117.621 0.000 0.0 02 A2 29156904.000 269.633 269.633 0.000 0.0 03 A3 6637623.000 60.164 60.164 0.000 0.0 04 A4 31525540.000 291.665 291.665 0.000 0.0 05 A5 33941872.000 314.141 314.141 0.000 0.0 06 A6 12063335.000 110.633 110.633 0.000 0.0 07 A7 13750599.000 126.327 126.327 0.000 0.0 08 A8 30868270.000 285.551 285.551 0.000 0.0 09 A9 20088456.000 185.280 185.280 0.000 0.0 097 A2 50680322.000 R 469.837 469.837 0.000 0.0 098 B2 33989594.000 314.585 314.585 0.000 0.0 099 C2 14296249.000 131.403 131.403 0.000 0.0 10 A10 16035970.000 147.585 147.585 0.000 0.0 100 D2 6697947.000 60.725 60.725 0.000 0.0 101 E2 2118276.000 18.127 18.127 0.000 0.0 102 F2 774487.000 5.627 5.627 0.000 0.0 103 G2 266483.000 0.902 0.902 0.000 0.0 104 H2 7412.000 R -1.508 -1.508 0.000 0.0 11 A11 16586703.000 152.708 152.708 0.000 0.0 12 A12 14733480.000 135.470 135.470 0.000 0.0 13 B1 35309548.000 326.863 326.863 0.000 0.0 14 B2 23882312.000 220.570 220.570 0.000 0.0 15 B3 13409260.000 123.152 123.152 0.000 0.0 16 B4 8145976.000 74.195 74.195 0.000 0.0 17 B5 29247336.000 270.474 270.474 0.000 0.0 18 B6 6016573.000 54.388 54.388 0.000 0.0 19 B7 17703368.000 163.095 163.095 0.000 0.0 20 B8 19692534.000 181.598 181.598 0.000 0.0 21 B9 16217575.000 149.274 149.274 0.000 0.0 22 B10 12367023.000 113.458 113.458 0.000 0.0 23 B11 3711687.000 32.948 32.948 0.000 0.0 24 B12 4801309.000 43.083 43.083 0.000 0.0 25 C1 15560951.000 143.167 143.167 0.000 0.0 26 C2 20661004.000 190.606 190.606 0.000 0.0 27 C3 31597050.000 292.330 292.330 0.000 0.0 28 C4 18248066.000 168.162 168.162 0.000 0.0 29 C5 10799317.000 98.875 98.875 0.000 0.0 30 C6 10132179.000 92.670 92.670 0.000 0.0 31 C7 7546503.000 68.619 68.619 0.000 0.0 32 C8 17883294.000 164.769 164.769 0.000 0.0 33 C9 17053766.000 157.052 157.052 0.000 0.0 34 C10 14803003.000 136.116 136.116 0.000 0.0 35 C11 3535895.000 31.313 31.313 0.000 0.0 36 C12 10549110.000 96.548 96.548 0.000 0.0 37 D1 28656816.000 264.981 264.981 0.000 0.0 38 D2 22237428.000 205.269 205.269 0.000 0.0 39 D3 15346543.000 141.172 141.172 0.000 0.0 40 D4 16155585.000 148.698 148.698 0.000 0.0 41 D5 13365123.000 122.742 122.742 0.000 0.0 42 D6 29742706.000 275.081 275.081 0.000 0.0 43 D7 11347259.000 103.972 103.972 0.000 0.0 44 D8 23582564.000 217.782 217.782 0.000 0.0 45 D9 17440940.000 160.654 160.654 0.000 0.0 46 D10 26892144.000 248.566 248.566 0.000 0.0 47 D11 4759886.000 42.698 42.698 0.000 0.0 48 D12 9329132.000 85.200 85.200 0.000 0.0 49 E1 32044182.000 296.489 296.489 0.000 0.0 50 E2 33567452.000 310.658 310.658 0.000 0.0 51 E3 21942298.000 202.524 202.524 0.000 0.0 52 E4 18471868.000 170.243 170.243 0.000 0.0 53 E5 15211899.000 139.920 139.920 0.000 0.0 54 E6 10640007.000 97.393 97.393 0.000 0.0 55 E7 20788720.000 191.794 191.794 0.000 0.0 56 E8 20248672.000 186.771 186.771 0.000 0.0 57 E9 17998288.000 165.838 165.838 0.000 0.0 58 E10 5806123.000 52.430 52.430 0.000 0.0 59 E11 7170897.000 65.125 65.125 0.000 0.0 60 E12 3481822.000 30.810 30.810 0.000 0.0 61 F1 30650954.000 283.530 283.530 0.000 0.0 62 F2 26831980.000 248.007 248.007 0.000 0.0 63 F3 12822788.000 117.697 117.697 0.000 0.0 64 F4 18642832.000 171.834 171.834 0.000 0.0 65 F5 14148807.000 130.031 130.031 0.000 0.0 66 F6 17756518.000 163.589 163.589 0.000 0.0 67 F7 12506397.000 114.754 114.754 0.000 0.0 68 F8 24220886.000 223.719 223.719 0.000 0.0 69 F9 21868776.000 201.840 201.840 0.000 0.0 70 F10 8678100.000 79.144 79.144 0.000 0.0 71 F11 2984495.000 26.184 26.184 0.000 0.0 72 F12 26398446.000 243.974 243.974 0.000 0.0 73 G1 14934855.000 137.343 137.343 0.000 0.0 74 G2 21420318.000 197.669 197.669 0.000 0.0 75 G3 16108749.000 148.262 148.262 0.000 0.0 76 G4 17497778.000 161.183 161.183 0.000 0.0 77 G5 26109182.000 241.283 241.283 0.000 0.0 78 G6 12731149.000 116.845 116.845 0.000 0.0 79 G7 19438714.000 179.237 179.237 0.000 0.0 80 G8 20347258.000 187.688 187.688 0.000 0.0 81 G9 16530366.000 152.184 152.184 0.000 0.0 82 G10 19228064.000 177.277 177.277 0.000 0.0 83 G11 2764438.000 24.137 24.137 0.000 0.0 84 G12 7966640.000 72.527 72.527 0.000 0.0 85 H1 15428695.000 141.936 141.936 0.000 0.0 86 H2 20037574.000 184.807 184.807 0.000 0.0 87 H3 15451481.000 142.148 142.148 0.000 0.0 88 H4 23591998.000 217.869 217.869 0.000 0.0 89 H5 24368862.000 225.095 225.095 0.000 0.0 90 H6 20395820.000 188.139 188.139 0.000 0.0 91 H7 17400090.000 160.274 160.274 0.000 0.0 92 H8 24170890.000 223.254 223.254 0.000 0.0 93 H9 18874742.000 173.991 173.991 0.000 0.0 94 H10 7120646.000 64.657 64.657 0.000 0.0 95 H11 17285102.000 159.204 159.204 0.000 0.0 96 H12 24686224.000 228.047 228.047 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: HiSeq661_TotalRNA_Conc_PlateReader; Date Last Saved: 10/14/2016 4:03:20 PM