##BLOCKS= 3 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 39779934 20816772 21271044 12639859 28259268 24124588 18995792 15657018 21192122 12498387 11138911 20054652 28130744 15062425 16991808 9248885 18411998 16143094 15257480 20939524 12960965 14452145 8230801 16621042 12871499 22105436 15341369 16642388 24898186 16715108 6943027 21832076 17802458 9455882 12598571 8117644 5702864 17756728 12285571 9818445 19525002 20567452 9054764 14421934 18717278 9755525 14305431 14351922 2022863 24554178 16097780 21480560 26759384 22246078 15052578 20060016 -121984 14390515 14994848 13840290 704252 19754020 13538970 26091102 24510234 19317466 11781073 17960376 -121913 13907787 15764911 11843869 256942 25563660 11262955 27368512 23481108 26331768 23591468 22766876 -121986 13474206 13883280 12615346 0 18662162 15791389 20238258 19836534 14219556 7117411 16515964 -122067 13974516 15662693 12359388 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 500.000 461.224 A1 39779934.000 39779934.000 0.000 0.0 02 250.000 323.376 B1 28130744.000 28130744.000 0.000 0.0 03 125.000 142.808 C1 12871499.000 12871499.000 0.000 0.0 04 62.500 57.980 D1 5702864.000 5702864.000 0.000 0.0 05 31.250 14.433 E1 2022863.000 2022863.000 0.000 0.0 06 15.625 -1.170 F1 704252.000 704252.000 0.000 0.0 07 7.813 -6.463 G1 256942.000 256942.000 0.000 0.0 Group Summaries MinStd Smallest standard value: 256942.000 Min(MeanValue) MaxStd Largest standard value: 39779934.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A2 20816772.000 236.827 236.827 0.000 0.0 02 B2 15062425.000 168.734 168.734 0.000 0.0 03 C2 22105436.000 252.076 252.076 0.000 0.0 04 D2 17756728.000 200.617 200.617 0.000 0.0 05 E2 24554178.000 281.053 281.053 0.000 0.0 06 F2 19754020.000 224.251 224.251 0.000 0.0 07 G2 25563660.000 292.999 292.999 0.000 0.0 08 H2 18662162.000 211.331 211.331 0.000 0.0 09 A3 21271044.000 242.203 242.203 0.000 0.0 10 B3 16991808.000 191.565 191.565 0.000 0.0 11 C3 15341369.000 172.035 172.035 0.000 0.0 12 D3 12285571.000 135.875 135.875 0.000 0.0 13 E3 16097780.000 180.986 180.986 0.000 0.0 14 F3 13538970.000 150.707 150.707 0.000 0.0 15 G3 11262955.000 123.774 123.774 0.000 0.0 16 H3 15791389.000 177.360 177.360 0.000 0.0 17 A4 12639859.000 140.067 140.067 0.000 0.0 18 B4 9248885.000 99.941 99.941 0.000 0.0 19 C4 16642388.000 187.430 187.430 0.000 0.0 20 D4 9818445.000 106.681 106.681 0.000 0.0 21 E4 21480560.000 244.682 244.682 0.000 0.0 22 F4 26091102.000 299.240 299.240 0.000 0.0 23 G4 27368512.000 314.356 314.356 0.000 0.0 24 H4 20238258.000 229.982 229.982 0.000 0.0 25 A5 28259268.000 324.897 324.897 0.000 0.0 26 B5 18411998.000 208.371 208.371 0.000 0.0 27 C5 24898186.000 285.124 285.124 0.000 0.0 28 D5 19525002.000 221.541 221.541 0.000 0.0 29 E5 26759384.000 307.148 307.148 0.000 0.0 30 F5 24510234.000 280.533 280.533 0.000 0.0 31 G5 23481108.000 268.355 268.355 0.000 0.0 32 H5 19836534.000 225.228 225.228 0.000 0.0 33 A6 24124588.000 275.970 275.970 0.000 0.0 34 B6 16143094.000 181.522 181.522 0.000 0.0 35 C6 16715108.000 188.291 188.291 0.000 0.0 36 D6 20567452.000 233.877 233.877 0.000 0.0 37 E6 22246078.000 253.741 253.741 0.000 0.0 38 F6 19317466.000 219.085 219.085 0.000 0.0 39 G6 26331768.000 302.088 302.088 0.000 0.0 40 H6 14219556.000 158.760 158.760 0.000 0.0 41 A7 18995792.000 215.279 215.279 0.000 0.0 42 B7 15257480.000 171.042 171.042 0.000 0.0 43 C7 6943027.000 72.655 72.655 0.000 0.0 44 D7 9054764.000 97.644 97.644 0.000 0.0 45 E7 15052578.000 168.618 168.618 0.000 0.0 46 F7 11781073.000 129.905 129.905 0.000 0.0 47 G7 23591468.000 269.661 269.661 0.000 0.0 48 H7 7117411.000 74.719 74.719 0.000 0.0 49 A8 15657018.000 175.770 175.770 0.000 0.0 50 B8 20939524.000 238.280 238.280 0.000 0.0 51 C8 21832076.000 248.842 248.842 0.000 0.0 52 D8 14421934.000 161.155 161.155 0.000 0.0 53 E8 20060016.000 227.872 227.872 0.000 0.0 54 F8 17960376.000 203.027 203.027 0.000 0.0 55 G8 22766876.000 259.903 259.903 0.000 0.0 56 H8 16515964.000 185.934 185.934 0.000 0.0 57 A9 21192122.000 241.269 241.269 0.000 0.0 58 B9 12960965.000 143.867 143.867 0.000 0.0 59 C9 17802458.000 201.158 201.158 0.000 0.0 60 D9 18717278.000 211.983 211.983 0.000 0.0 61 E9 -121984.000 R -10.947 -10.947 0.000 0.0 62 F9 -121913.000 R -10.947 -10.947 0.000 0.0 63 G9 -121986.000 R -10.947 -10.947 0.000 0.0 64 H9 -122067.000 R -10.948 -10.948 0.000 0.0 65 A10 12498387.000 138.393 138.393 0.000 0.0 66 B10 14452145.000 161.513 161.513 0.000 0.0 67 C10 9455882.000 102.390 102.390 0.000 0.0 68 D10 9755525.000 105.936 105.936 0.000 0.0 69 E10 14390515.000 160.783 160.783 0.000 0.0 70 F10 13907787.000 155.071 155.071 0.000 0.0 71 G10 13474206.000 149.940 149.940 0.000 0.0 72 H10 13974516.000 155.861 155.861 0.000 0.0 73 A11 11138911.000 122.306 122.306 0.000 0.0 74 B11 8230801.000 87.894 87.894 0.000 0.0 75 C11 12598571.000 139.579 139.579 0.000 0.0 76 D11 14305431.000 159.777 159.777 0.000 0.0 77 E11 14994848.000 167.935 167.935 0.000 0.0 78 F11 15764911.000 177.047 177.047 0.000 0.0 79 G11 13883280.000 154.781 154.781 0.000 0.0 80 H11 15662693.000 175.837 175.837 0.000 0.0 81 A12 20054652.000 227.809 227.809 0.000 0.0 82 B12 16621042.000 187.178 187.178 0.000 0.0 83 C12 8117644.000 86.555 86.555 0.000 0.0 84 D12 14351922.000 160.327 160.327 0.000 0.0 85 E12 13840290.000 154.272 154.272 0.000 0.0 86 F12 11843869.000 130.648 130.648 0.000 0.0 87 G12 12615346.000 139.777 139.777 0.000 0.0 88 H12 12359388.000 136.748 136.748 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: HiSeq803-HiSeq823_TotalRNAConc_02-02-2017; Date Last Saved: 2/2/2017 3:27:53 PM