##BLOCKS= 7 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 46130 13610731 13578432 -11583 -11677 -11576 -11590 -11630 -11482 -11536 -11463 -11562 585277 5365977 5137674 -11537 -11623 -11206 -11532 -11581 -11429 -11522 -11504 -11454 564043 2667346 3068917 -11530 -11551 -11561 -11530 -11556 -11554 -11477 -11566 -11695 192051 240106 276345 -11456 -11653 -11503 -11613 -11550 -11264 -11566 -11521 -11599 982059 114605 171651 -11572 -11501 -11483 -11525 -11600 -11558 -11558 -11474 -11474 386748 144163 119466 -11412 -11576 -11519 -11566 -11325 -11339 -11535 -11477 -11542 643090 8003 8791 -11558 -11546 -11336 -11502 -11607 -11558 -11642 -11423 -11561 487051 421 -421 -7820 -10475 -11435 -11491 -11517 -11594 -11575 -11549 -11610 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 3358602 6511949 3376071 9418314 7084676 3706103 12057403 8109657 11592478 8939712 12575124 8135056 9740021 4363937 4873922 6791946 12111037 10374607 3529931 11553208 7515385 12288012 9079462 13058730 4449590 6620773 2572842 7833429 11613957 9509747 3992470 10665372 10321001 15232572 11550316 12819253 4115871 5499748 6480225 7633399 10511886 9883080 3587162 10680938 9323026 10712490 9830942 5503334 4324778 5909128 5602242 9197720 7786155 10636595 5957089 8200139 15063489 12093056 12872495 11325584 4221466 5345417 9297533 6565096 7115696 10407958 12200015 8187366 11452494 14441899 4686314 8536997 6146140 8690827 8996943 10558860 2881773 3187651 8955948 8216152 9015823 11704723 12578474 9078022 1108035 1965357 4903407 5296001 4048598 13841995 12890341 10524118 9447582 8775575 3932907 10893656 ~End Plate: Plate3 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 8460170 8247281 9850530 6078564 13805170 7280038 9095788 12316651 10718168 10955401 13521255 11348249 12419947 11460815 5101004 9817660 10940465 5267123 9094210 9073455 10016554 10541322 12448046 12252231 11643101 14139067 5324961 10053801 9570858 13539591 6022104 8553209 11685436 9091628 8666956 10300731 12368091 12544602 11535502 8873311 11120722 9678906 10409245 12784893 9820390 11671693 9749347 5973002 14028435 6875313 12017191 13096671 10886550 9891201 8377174 8956787 13783305 10461370 9968657 5933797 9104238 10640591 9348429 9599362 9079882 11037240 7266376 10804779 2977814 11005877 4830676 10218401 13065386 11279361 12471085 10177994 6139448 10073606 12620650 11796693 10880457 12344366 12864649 12392900 13915449 3245130 12511907 4825500 8684881 9651093 23986402 16075953 12086475 11961669 12728087 9676566 ~End Plate: Plate4 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 11053721 9152690 9451598 6731860 9996489 10354486 7181793 11487857 2957218 4275276 4911423 11710789 8109846 9741788 10191371 3022419 12796167 7287741 9203054 9550680 7117648 3068836 5837523 11922071 10765936 10659441 7257012 8979184 11446432 11499473 5878541 7619075 4106920 5631003 3433400 8340042 10255854 9101376 11898915 6905459 8895098 12465841 7343596 11992307 5958230 10852252 11789835 8368092 12408956 6728690 13557537 10949978 9387045 11240499 8319437 8318628 8619526 8106295 11319741 8695802 8838019 12356897 9754474 11912817 10139274 12561376 6166431 11266404 10680867 11701718 5031949 6678968 10088992 11704127 8350148 10023237 9367393 8167823 10859996 5177574 9081284 8224232 6724838 4068674 13327733 5171731 10514367 17609008 12545989 9871551 6563862 11674757 8370948 4132398 12895226 6532436 ~End Plate: Plate5 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 8723503 11596973 5718435 8728756 10000473 6382172 8745214 11741814 11871736 12452272 14127933 10013771 9324266 6941424 7166147 10534881 12881961 11457224 9403990 9889776 9730921 10725011 10097555 7073593 4147662 14224813 11604787 8844136 10990946 11217124 9884090 9632094 10721937 13857544 11621277 8571817 7807632 5558599 12620940 15170256 8519997 11679493 10870144 8542272 8744369 7765115 12739181 10028754 6165456 7975176 9623676 9797256 11395272 9698079 10624785 4245204 10339502 14309021 11971268 12691642 11584341 10837706 4061708 9663953 9301679 6010306 11848941 9571794 9484485 8625175 11305846 13660770 4309419 7299286 11729119 9459038 9494831 9668056 8242106 9550556 6030003 11875397 6902799 13928359 12406594 1583355 11257045 5936340 5816386 11713694 12282182 15095498 12793885 13483645 17368706 8660101 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 50.000 51.834 A2 13610731.000 13594581.500 22838.842 0.2 51.718 A3 13578432.000 02 25.000 22.068 B2 5365977.000 5251825.500 161434.599 3.1 21.244 B3 5137674.000 03 12.500 12.325 C2 2667346.000 2868131.500 283953.577 9.9 13.775 C3 3068917.000 04 6.250 3.562 D2 240106.000 258225.500 25624.843 9.9 3.693 D3 276345.000 05 3.125 3.109 E2 114605.000 143128.000 40337.613 28.2 3.315 E3 171651.000 06 1.563 3.216 F2 144163.000 131814.500 17463.416 13.2 3.127 F3 119466.000 07 0.781 2.724 G2 8003.000 8397.000 557.200 6.6 2.727 G3 8791.000 Group Summaries MinStd Smallest standard value: 8397.000 Min(MeanValue) MaxStd Largest standard value: 13594581.500 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 3358602.000 14.821 14.821 0.000 0.0 02 B1 9740021.000 37.860 37.860 0.000 0.0 03 C1 4449590.000 18.760 18.760 0.000 0.0 04 D1 4115871.000 17.555 17.555 0.000 0.0 05 E1 4324778.000 18.309 18.309 0.000 0.0 06 F1 4221466.000 17.936 17.936 0.000 0.0 07 G1 6146140.000 24.885 24.885 0.000 0.0 08 H1 1108035.000 6.696 6.696 0.000 0.0 09 A2 6511949.000 26.206 26.206 0.000 0.0 097 A1 8460170.000 33.239 33.239 0.000 0.0 098 B1 12419947.000 47.535 47.535 0.000 0.0 099 C1 11643101.000 44.731 44.731 0.000 0.0 10 B2 4363937.000 18.451 18.451 0.000 0.0 100 D1 12368091.000 47.348 47.348 0.000 0.0 101 E1 14028435.000 R 53.342 53.342 0.000 0.0 102 F1 9104238.000 35.565 35.565 0.000 0.0 103 G1 13065386.000 49.866 49.866 0.000 0.0 104 H1 13915449.000 R 52.935 52.935 0.000 0.0 105 A2 8247281.000 32.471 32.471 0.000 0.0 106 B2 11460815.000 44.073 44.073 0.000 0.0 107 C2 14139067.000 R 53.742 53.742 0.000 0.0 108 D2 12544602.000 47.985 47.985 0.000 0.0 109 E2 6875313.000 27.517 27.517 0.000 0.0 11 C2 6620773.000 26.598 26.598 0.000 0.0 110 F2 10640591.000 41.111 41.111 0.000 0.0 111 G2 11279361.000 43.417 43.417 0.000 0.0 112 H2 3245130.000 14.411 14.411 0.000 0.0 113 A3 9850530.000 38.259 38.259 0.000 0.0 114 B3 5101004.000 21.112 21.112 0.000 0.0 115 C3 5324961.000 21.920 21.920 0.000 0.0 116 D3 11535502.000 44.342 44.342 0.000 0.0 117 E3 12017191.000 46.081 46.081 0.000 0.0 118 F3 9348429.000 36.446 36.446 0.000 0.0 119 G3 12471085.000 47.720 47.720 0.000 0.0 12 D2 5499748.000 22.551 22.551 0.000 0.0 120 H3 12511907.000 47.867 47.867 0.000 0.0 121 A4 6078564.000 24.641 24.641 0.000 0.0 122 B4 9817660.000 38.140 38.140 0.000 0.0 123 C4 10053801.000 38.993 38.993 0.000 0.0 124 D4 8873311.000 34.731 34.731 0.000 0.0 125 E4 13096671.000 49.979 49.979 0.000 0.0 126 F4 9599362.000 37.352 37.352 0.000 0.0 127 G4 10177994.000 39.441 39.441 0.000 0.0 128 H4 4825500.000 20.117 20.117 0.000 0.0 129 A5 13805170.000 R 52.536 52.536 0.000 0.0 13 E2 5909128.000 24.029 24.029 0.000 0.0 130 B5 10940465.000 42.194 42.194 0.000 0.0 131 C5 9570858.000 37.249 37.249 0.000 0.0 132 D5 11120722.000 42.845 42.845 0.000 0.0 133 E5 10886550.000 41.999 41.999 0.000 0.0 134 F5 9079882.000 35.477 35.477 0.000 0.0 135 G5 6139448.000 24.861 24.861 0.000 0.0 136 H5 8684881.000 34.051 34.051 0.000 0.0 137 A6 7280038.000 28.979 28.979 0.000 0.0 138 B6 5267123.000 21.711 21.711 0.000 0.0 139 C6 13539591.000 51.578 51.578 0.000 0.0 14 F2 5345417.000 21.994 21.994 0.000 0.0 140 D6 9678906.000 37.639 37.639 0.000 0.0 141 E6 9891201.000 38.406 38.406 0.000 0.0 142 F6 11037240.000 42.543 42.543 0.000 0.0 143 G6 10073606.000 39.064 39.064 0.000 0.0 144 H6 9651093.000 37.539 37.539 0.000 0.0 145 A7 9095788.000 35.534 35.534 0.000 0.0 146 B7 9094210.000 35.528 35.528 0.000 0.0 147 C7 6022104.000 24.437 24.437 0.000 0.0 148 D7 10409245.000 40.276 40.276 0.000 0.0 149 E7 8377174.000 32.940 32.940 0.000 0.0 15 G2 8690827.000 34.072 34.072 0.000 0.0 150 F7 7266376.000 28.929 28.929 0.000 0.0 151 G7 12620650.000 48.260 48.260 0.000 0.0 152 H7 23986402.000 R 89.294 89.294 0.000 0.0 153 A8 12316651.000 47.162 47.162 0.000 0.0 154 B8 9073455.000 35.453 35.453 0.000 0.0 155 C8 8553209.000 33.575 33.575 0.000 0.0 156 D8 12784893.000 48.853 48.853 0.000 0.0 157 E8 8956787.000 35.032 35.032 0.000 0.0 158 F8 10804779.000 41.704 41.704 0.000 0.0 159 G8 11796693.000 45.285 45.285 0.000 0.0 16 H2 1965357.000 9.791 9.791 0.000 0.0 160 H8 16075953.000 R 60.735 60.735 0.000 0.0 161 A9 10718168.000 41.391 41.391 0.000 0.0 162 B9 10016554.000 38.858 38.858 0.000 0.0 163 C9 11685436.000 44.883 44.883 0.000 0.0 164 D9 9820390.000 38.150 38.150 0.000 0.0 165 E9 13783305.000 R 52.457 52.457 0.000 0.0 166 F9 2977814.000 13.446 13.446 0.000 0.0 167 G9 10880457.000 41.977 41.977 0.000 0.0 168 H9 12086475.000 46.331 46.331 0.000 0.0 169 A10 10955401.000 42.248 42.248 0.000 0.0 17 A3 3376071.000 14.884 14.884 0.000 0.0 170 B10 10541322.000 40.753 40.753 0.000 0.0 171 C10 9091628.000 35.519 35.519 0.000 0.0 172 D10 11671693.000 44.834 44.834 0.000 0.0 173 E10 10461370.000 40.464 40.464 0.000 0.0 174 F10 11005877.000 42.430 42.430 0.000 0.0 175 G10 12344366.000 47.262 47.262 0.000 0.0 176 H10 11961669.000 45.881 45.881 0.000 0.0 177 A11 13521255.000 51.511 51.511 0.000 0.0 178 B11 12448046.000 47.637 47.637 0.000 0.0 179 C11 8666956.000 33.986 33.986 0.000 0.0 18 B3 4873922.000 20.292 20.292 0.000 0.0 180 D11 9749347.000 37.894 37.894 0.000 0.0 181 E11 9968657.000 38.685 38.685 0.000 0.0 182 F11 4830676.000 20.136 20.136 0.000 0.0 183 G11 12864649.000 49.141 49.141 0.000 0.0 184 H11 12728087.000 48.648 48.648 0.000 0.0 185 A12 11348249.000 43.666 43.666 0.000 0.0 186 B12 12252231.000 46.930 46.930 0.000 0.0 187 C12 10300731.000 39.884 39.884 0.000 0.0 188 D12 5973002.000 24.260 24.260 0.000 0.0 189 E12 5933797.000 24.118 24.118 0.000 0.0 19 C3 2572842.000 11.984 11.984 0.000 0.0 190 F12 10218401.000 39.587 39.587 0.000 0.0 191 G12 12392900.000 47.438 47.438 0.000 0.0 192 H12 9676566.000 37.631 37.631 0.000 0.0 193 A1 11053721.000 42.603 42.603 0.000 0.0 194 B1 8109846.000 31.974 31.974 0.000 0.0 195 C1 10765936.000 41.564 41.564 0.000 0.0 196 D1 10255854.000 39.722 39.722 0.000 0.0 197 E1 12408956.000 47.496 47.496 0.000 0.0 198 F1 8838019.000 34.603 34.603 0.000 0.0 199 G1 10088992.000 39.120 39.120 0.000 0.0 20 D3 6480225.000 26.091 26.091 0.000 0.0 200 H1 13327733.000 50.813 50.813 0.000 0.0 201 A2 9152690.000 35.739 35.739 0.000 0.0 202 B2 9741788.000 37.866 37.866 0.000 0.0 203 C2 10659441.000 41.179 41.179 0.000 0.0 204 D2 9101376.000 35.554 35.554 0.000 0.0 205 E2 6728690.000 26.988 26.988 0.000 0.0 206 F2 12356897.000 47.308 47.308 0.000 0.0 207 G2 11704127.000 44.951 44.951 0.000 0.0 208 H2 5171731.000 21.367 21.367 0.000 0.0 209 A3 9451598.000 36.819 36.819 0.000 0.0 21 E3 5602242.000 22.921 22.921 0.000 0.0 210 B3 10191371.000 39.489 39.489 0.000 0.0 211 C3 7257012.000 28.895 28.895 0.000 0.0 212 D3 11898915.000 45.654 45.654 0.000 0.0 213 E3 13557537.000 51.642 51.642 0.000 0.0 214 F3 9754474.000 37.912 37.912 0.000 0.0 215 G3 8350148.000 32.842 32.842 0.000 0.0 216 H3 10514367.000 40.656 40.656 0.000 0.0 217 A4 6731860.000 26.999 26.999 0.000 0.0 218 B4 3022419.000 13.607 13.607 0.000 0.0 219 C4 8979184.000 35.113 35.113 0.000 0.0 22 F3 9297533.000 36.262 36.262 0.000 0.0 220 D4 6905459.000 27.626 27.626 0.000 0.0 221 E4 10949978.000 42.228 42.228 0.000 0.0 222 F4 11912817.000 45.704 45.704 0.000 0.0 223 G4 10023237.000 38.882 38.882 0.000 0.0 224 H4 17609008.000 R 66.270 66.270 0.000 0.0 225 A5 9996489.000 38.786 38.786 0.000 0.0 226 B5 12796167.000 48.894 48.894 0.000 0.0 227 C5 11446432.000 44.021 44.021 0.000 0.0 228 D5 8895098.000 34.809 34.809 0.000 0.0 229 E5 9387045.000 36.586 36.586 0.000 0.0 23 G3 8996943.000 35.177 35.177 0.000 0.0 230 F5 10139274.000 39.301 39.301 0.000 0.0 231 G5 9367393.000 36.515 36.515 0.000 0.0 232 H5 12545989.000 47.990 47.990 0.000 0.0 233 A6 10354486.000 40.078 40.078 0.000 0.0 234 B6 7287741.000 29.006 29.006 0.000 0.0 235 C6 11499473.000 44.212 44.212 0.000 0.0 236 D6 12465841.000 47.701 47.701 0.000 0.0 237 E6 11240499.000 43.277 43.277 0.000 0.0 238 F6 12561376.000 48.046 48.046 0.000 0.0 239 G6 8167823.000 32.184 32.184 0.000 0.0 24 H3 4903407.000 20.398 20.398 0.000 0.0 240 H6 9871551.000 38.335 38.335 0.000 0.0 241 A7 7181793.000 28.624 28.624 0.000 0.0 242 B7 9203054.000 35.921 35.921 0.000 0.0 243 C7 5878541.000 23.919 23.919 0.000 0.0 244 D7 7343596.000 29.208 29.208 0.000 0.0 245 E7 8319437.000 32.731 32.731 0.000 0.0 246 F7 6166431.000 24.958 24.958 0.000 0.0 247 G7 10859996.000 41.903 41.903 0.000 0.0 248 H7 6563862.000 26.393 26.393 0.000 0.0 249 A8 11487857.000 44.170 44.170 0.000 0.0 25 A4 9418314.000 36.698 36.698 0.000 0.0 250 B8 9550680.000 37.176 37.176 0.000 0.0 251 C8 7619075.000 30.203 30.203 0.000 0.0 252 D8 11992307.000 45.991 45.991 0.000 0.0 253 E8 8318628.000 32.728 32.728 0.000 0.0 254 F8 11266404.000 43.371 43.371 0.000 0.0 255 G8 5177574.000 21.388 21.388 0.000 0.0 256 H8 11674757.000 44.845 44.845 0.000 0.0 257 A9 2957218.000 13.372 13.372 0.000 0.0 258 B9 7117648.000 28.392 28.392 0.000 0.0 259 C9 4106920.000 17.523 17.523 0.000 0.0 26 B4 6791946.000 27.216 27.216 0.000 0.0 260 D9 5958230.000 24.206 24.206 0.000 0.0 261 E9 8619526.000 33.815 33.815 0.000 0.0 262 F9 10680867.000 41.257 41.257 0.000 0.0 263 G9 9081284.000 35.482 35.482 0.000 0.0 264 H9 8370948.000 32.917 32.917 0.000 0.0 265 A10 4275276.000 18.130 18.130 0.000 0.0 266 B10 3068836.000 13.775 13.775 0.000 0.0 267 C10 5631003.000 23.025 23.025 0.000 0.0 268 D10 10852252.000 41.875 41.875 0.000 0.0 269 E10 8106295.000 31.962 31.962 0.000 0.0 27 C4 7833429.000 30.977 30.977 0.000 0.0 270 F10 11701718.000 44.942 44.942 0.000 0.0 271 G10 8224232.000 32.387 32.387 0.000 0.0 272 H10 4132398.000 17.615 17.615 0.000 0.0 273 A11 4911423.000 20.427 20.427 0.000 0.0 274 B11 5837523.000 23.771 23.771 0.000 0.0 275 C11 3433400.000 15.091 15.091 0.000 0.0 276 D11 11789835.000 45.260 45.260 0.000 0.0 277 E11 11319741.000 43.563 43.563 0.000 0.0 278 F11 5031949.000 20.862 20.862 0.000 0.0 279 G11 6724838.000 26.974 26.974 0.000 0.0 28 D4 7633399.000 30.254 30.254 0.000 0.0 280 H11 12895226.000 49.251 49.251 0.000 0.0 281 A12 11710789.000 44.975 44.975 0.000 0.0 282 B12 11922071.000 45.738 45.738 0.000 0.0 283 C12 8340042.000 32.806 32.806 0.000 0.0 284 D12 8368092.000 32.907 32.907 0.000 0.0 285 E12 8695802.000 34.090 34.090 0.000 0.0 286 F12 6678968.000 26.809 26.809 0.000 0.0 287 G12 4068674.000 17.385 17.385 0.000 0.0 288 H12 6532436.000 26.279 26.279 0.000 0.0 289 A1 8723503.000 34.190 34.190 0.000 0.0 29 E4 9197720.000 35.902 35.902 0.000 0.0 290 B1 9324266.000 36.359 36.359 0.000 0.0 291 C1 4147662.000 17.670 17.670 0.000 0.0 292 D1 7807632.000 30.883 30.883 0.000 0.0 293 E1 6165456.000 24.955 24.955 0.000 0.0 294 F1 11584341.000 44.519 44.519 0.000 0.0 295 G1 4309419.000 18.254 18.254 0.000 0.0 296 H1 12406594.000 47.487 47.487 0.000 0.0 297 A2 11596973.000 44.564 44.564 0.000 0.0 298 B2 6941424.000 27.756 27.756 0.000 0.0 299 C2 14224813.000 R 54.051 54.051 0.000 0.0 30 F4 6565096.000 26.397 26.397 0.000 0.0 300 D2 5558599.000 22.764 22.764 0.000 0.0 301 E2 7975176.000 31.488 31.488 0.000 0.0 302 F2 10837706.000 41.823 41.823 0.000 0.0 303 G2 7299286.000 29.048 29.048 0.000 0.0 304 H2 1583355.000 8.412 8.412 0.000 0.0 305 A3 5718435.000 23.341 23.341 0.000 0.0 306 B3 7166147.000 28.567 28.567 0.000 0.0 307 C3 11604787.000 44.592 44.592 0.000 0.0 308 D3 12620940.000 48.261 48.261 0.000 0.0 309 E3 9623676.000 37.440 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E12 11325584.000 43.584 43.584 0.000 0.0 94 F12 8536997.000 33.517 33.517 0.000 0.0 95 G12 9078022.000 35.470 35.470 0.000 0.0 96 H12 10893656.000 42.025 42.025 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: HiSeq878_Plates_1-4_Libraries; Date Last Saved: 8/28/2017 2:27:06 PM