##BLOCKS= 5 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 42900 106315 -36842 85245 -22956 -541 -15727 -25156 -9230 61516 103223 39100 53906 17126 152461 24627 38656 65988 145615 34160 154646 28363 47088 625460 10467 13981 31715 -4454 8985 10573 15291 24934 18626 52603 21418 239193 19595579 13736041 8633386 5813119 3746076 2548339 1769408 1089772 559299 333469 226637 0 -44270 -45795 -45715 -44473 -45211 -43528 -45641 -45740 -45386 -45803 -43077 -45806 -45797 -45715 -45730 -45790 -45753 -45758 -45650 -43364 -45045 -45623 -45734 -45170 -45776 -45742 -45793 -45634 -45702 -45824 -45736 -45834 -45334 -45702 -45748 -45743 -45757 -45810 -43955 -45368 -45836 -45742 -45848 -41136 -45758 -45797 -44548 -45424 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 31704 35389 151545 11385309 1037 1013 982 992 965 1493 1058 1120 398372 12287 370722 4598011 993 1770 1093 1081 1041 1129 1051 1170 479326 597003 289894 6723322 1104 1094 1161 1056 1056 1393 1013 982 571848 24935 16749 6268255 1090 943 1003 1079 1773 991 1027 984 1648769 1817326 7002 714198 1105 1096 1124 961 1095 2337 951 1048 644720 2644177 64437 28031032 1157 1169 1077 1146 1678 1128 1059 1045 1121990 5984 6596 11924580 1114 1187 1054 6035 1028 953 1073 1127 699060 184233 35721 26188402 1154 1116 3112 959 1051 18983 1072 1046 ~End Plate: Plate3 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 1021 11514544 237693 26209910 14828 18262 52942 15397 10606617 22402404 4230052 20513 1086 17715 453591 9859231 24468684 3114448 16828 1549290 561258 9514452 14481960 11258148 1320 1027786 11688242 9821367 1050810 4193075 4173276 8882201 484512 5467841 5554911 2425352 982 3747085 3569583 7468432 1976001 11782929 13064429 29475956 1913915 2230704 21365208 28000542 947 6185366 22342076 9883895 292373 17666286 11221497 8117515 7026067 33094226 25021270 6166649 1024 15733913 8054755 16706833 3197904 1353227 12358335 7811830 30661580 1424798 4485468 2013782 1015 5066931 30735588 13204530 24390820 883736 40234748 25271980 9154886 9964515 7066559 3769387 4513 6862341 23726022 8780604 3942400 14983463 14848148 14447987 27044756 22272 9295945 8413559 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 100.000 98.603 D1 19595579.000 19595579.000 0.000 0.0 02 66.667 69.283 D2 13736041.000 13736041.000 0.000 0.0 03 44.444 43.750 D3 8633386.000 8633386.000 0.000 0.0 04 29.630 29.638 D4 5813119.000 5813119.000 0.000 0.0 05 19.753 19.295 D5 3746076.000 3746076.000 0.000 0.0 06 13.169 13.302 D6 2548339.000 2548339.000 0.000 0.0 07 8.779 9.404 D7 1769408.000 1769408.000 0.000 0.0 08 5.853 6.003 D8 1089772.000 1089772.000 0.000 0.0 09 3.902 3.349 D9 559299.000 559299.000 0.000 0.0 10 2.601 2.219 D10 333469.000 333469.000 0.000 0.0 11 1.734 1.684 D11 226637.000 226637.000 0.000 0.0 Group Summaries MinStd Smallest standard value: 226637.000 Min(MeanValue) MaxStd Largest standard value: 19595579.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A4 11385309.000 57.520 57.520 0.000 0.0 02 B4 4598011.000 23.558 23.558 0.000 0.0 03 C4 6723322.000 34.193 34.193 0.000 0.0 04 D4 6268255.000 31.916 31.916 0.000 0.0 05 E4 714198.000 4.124 4.124 0.000 0.0 06 F4 28031032.000 R 140.813 140.813 0.000 0.0 07 G4 11924580.000 60.219 60.219 0.000 0.0 08 H4 26188402.000 R 131.593 131.593 0.000 0.0 09 A2 11514544.000 58.167 58.167 0.000 0.0 10 B2 17715.000 R 0.639 0.639 0.000 0.0 11 C2 1027786.000 5.693 5.693 0.000 0.0 12 D2 3747085.000 19.300 19.300 0.000 0.0 13 E2 6185366.000 31.501 31.501 0.000 0.0 14 F2 15733913.000 79.280 79.280 0.000 0.0 15 G2 5066931.000 25.904 25.904 0.000 0.0 16 H2 6862341.000 34.888 34.888 0.000 0.0 17 A3 237693.000 1.740 1.740 0.000 0.0 18 B3 453591.000 2.820 2.820 0.000 0.0 19 C3 11688242.000 59.036 59.036 0.000 0.0 20 D3 3569583.000 18.412 18.412 0.000 0.0 21 E3 22342076.000 R 112.346 112.346 0.000 0.0 22 F3 8054755.000 40.855 40.855 0.000 0.0 23 G3 30735588.000 R 154.346 154.346 0.000 0.0 24 H3 23726022.000 R 119.271 119.271 0.000 0.0 25 A4 26209910.000 R 131.700 131.700 0.000 0.0 26 B4 9859231.000 49.884 49.884 0.000 0.0 27 C4 9821367.000 49.695 49.695 0.000 0.0 28 D4 7468432.000 37.921 37.921 0.000 0.0 29 E4 9883895.000 50.008 50.008 0.000 0.0 30 F4 16706833.000 84.148 84.148 0.000 0.0 31 G4 13204530.000 66.624 66.624 0.000 0.0 32 H4 8780604.000 44.487 44.487 0.000 0.0 33 A5 14828.000 R 0.625 0.625 0.000 0.0 34 B5 24468684.000 R 122.987 122.987 0.000 0.0 35 C5 1050810.000 5.808 5.808 0.000 0.0 36 D5 1976001.000 10.438 10.438 0.000 0.0 37 E5 292373.000 2.013 2.013 0.000 0.0 38 F5 3197904.000 16.552 16.552 0.000 0.0 39 G5 24390820.000 R 122.598 122.598 0.000 0.0 40 H5 3942400.000 20.277 20.277 0.000 0.0 41 A6 18262.000 R 0.642 0.642 0.000 0.0 42 B6 3114448.000 16.135 16.135 0.000 0.0 43 C6 4193075.000 21.532 21.532 0.000 0.0 44 D6 11782929.000 59.510 59.510 0.000 0.0 45 E6 17666286.000 88.949 88.949 0.000 0.0 46 F6 1353227.000 7.322 7.322 0.000 0.0 47 G6 883736.000 4.972 4.972 0.000 0.0 48 H6 14983463.000 75.525 75.525 0.000 0.0 49 A7 52942.000 R 0.815 0.815 0.000 0.0 50 B7 16828.000 R 0.635 0.635 0.000 0.0 51 C7 4173276.000 21.433 21.433 0.000 0.0 52 D7 13064429.000 65.923 65.923 0.000 0.0 53 E7 11221497.000 56.701 56.701 0.000 0.0 54 F7 12358335.000 62.389 62.389 0.000 0.0 55 G7 40234748.000 R 201.878 201.878 0.000 0.0 56 H7 14848148.000 74.848 74.848 0.000 0.0 57 A8 15397.000 R 0.627 0.627 0.000 0.0 58 B8 1549290.000 8.303 8.303 0.000 0.0 59 C8 8882201.000 44.995 44.995 0.000 0.0 60 D8 29475956.000 R 148.043 148.043 0.000 0.0 61 E8 8117515.000 41.169 41.169 0.000 0.0 62 F8 7811830.000 39.639 39.639 0.000 0.0 63 G8 25271980.000 R 127.007 127.007 0.000 0.0 64 H8 14447987.000 72.846 72.846 0.000 0.0 65 A9 10606617.000 53.624 53.624 0.000 0.0 66 B9 561258.000 3.359 3.359 0.000 0.0 67 C9 484512.000 2.975 2.975 0.000 0.0 68 D9 1913915.000 10.127 10.127 0.000 0.0 69 E9 7026067.000 35.708 35.708 0.000 0.0 70 F9 30661580.000 R 153.976 153.976 0.000 0.0 71 G9 9154886.000 46.360 46.360 0.000 0.0 72 H9 27044756.000 R 135.878 135.878 0.000 0.0 73 A10 22402404.000 R 112.648 112.648 0.000 0.0 74 B10 9514452.000 48.159 48.159 0.000 0.0 75 C10 5467841.000 27.910 27.910 0.000 0.0 76 D10 2230704.000 11.712 11.712 0.000 0.0 77 E10 33094226.000 R 166.148 166.148 0.000 0.0 78 F10 1424798.000 7.680 7.680 0.000 0.0 79 G10 9964515.000 50.411 50.411 0.000 0.0 80 H10 22272.000 R 0.662 0.662 0.000 0.0 81 A11 4230052.000 21.717 21.717 0.000 0.0 82 B11 14481960.000 73.016 73.016 0.000 0.0 83 C11 5554911.000 28.346 28.346 0.000 0.0 84 D11 21365208.000 R 107.458 107.458 0.000 0.0 85 E11 25021270.000 R 125.753 125.753 0.000 0.0 86 F11 4485468.000 22.995 22.995 0.000 0.0 87 G11 7066559.000 35.910 35.910 0.000 0.0 88 H11 9295945.000 47.066 47.066 0.000 0.0 89 A12 20513.000 R 0.653 0.653 0.000 0.0 90 B12 11258148.000 56.884 56.884 0.000 0.0 91 C12 2425352.000 12.686 12.686 0.000 0.0 92 D12 28000542.000 R 140.660 140.660 0.000 0.0 93 E12 6166649.000 31.407 31.407 0.000 0.0 94 F12 2013782.000 10.627 10.627 0.000 0.0 95 G12 3769387.000 19.412 19.412 0.000 0.0 96 H12 8413559.000 42.650 42.650 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: HiSeq_1316_DNA_Conc_04-26-2018; Date Last Saved: 4/26/2018 11:18:46 AM