##BLOCKS= 5 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 375914 262273 10767 19725003 -19723 -19813 -19849 -19797 -19795 -19814 -19781 5511 384031 7430 4104 9545069 -19836 -19771 -19714 -19806 -19798 -19738 -19675 -8968 174223 -14427 -9089 4925832 -19731 -19660 -19801 -19807 -19742 -19862 -19745 -6511 430810 -15202 -8598 2076820 -19767 -19824 -19786 -19821 -19726 -19699 -19694 -4669 436943 41644 -4224 801310 -19728 -19750 -19828 -19758 -19820 -19717 -19796 59918 343532 55289 -11882 358002 -19713 -19651 -19740 -19826 -19761 -19745 -19767 67789 21801 353613 -2467 162627 -19811 -19749 -19736 -19793 -9471 -19742 -19736 23229 -14563 245465 -12055 0 -19892 -19781 -19805 -19826 -19813 -19737 -19918 76530 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 1551951 2158176 2561763 2697144 3676365 4966391 1062516 2073709 2401142 3056521 2246573 2482193 1540721 1547113 4904388 1837121 1669934 2171953 1228279 2719207 1647226 2342815 2528237 2355542 1837332 1046809 2469415 2209713 7674062 1682566 1666329 3130523 1619434 9206457 357020 2251980 1430379 1527889 3123768 5774399 3709865 2242280 2877483 7218791 5164358 1537921 1712656 6264001 740427 1843261 8489010 3659930 3648964 4782629 1660379 10607101 2228772 1695028 5687698 1321 2074248 3293514 2334241 8224105 3378719 1097237 2561387 4701423 1668952 3082476 10776274 1401 3819637 3571870 6661179 2728792 4818703 1276543 2015814 6244621 4011231 2223427 1346840 1390 2273985 4103254 7649281 1630400 3853221 1029939 793267 3735288 2179769 1066528 1904853 1422 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 50.000 50.105 A4 19725003.000 19725003.000 0.000 0.0 02 25.000 24.623 B4 9545069.000 9545069.000 0.000 0.0 03 12.500 13.061 C4 4925832.000 4925832.000 0.000 0.0 04 6.250 5.929 D4 2076820.000 2076820.000 0.000 0.0 05 3.125 2.736 E4 801310.000 801310.000 0.000 0.0 06 1.563 1.627 F4 358002.000 358002.000 0.000 0.0 07 0.781 1.138 G4 162627.000 162627.000 0.000 0.0 Group Summaries MinStd Smallest standard value: 162627.000 Min(MeanValue) MaxStd Largest standard value: 19725003.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 1551951.000 4.615 4.615 0.000 0.0 02 A2 2158176.000 6.133 6.133 0.000 0.0 03 A3 2561763.000 7.143 7.143 0.000 0.0 04 A4 2697144.000 7.482 7.482 0.000 0.0 05 A5 3676365.000 9.933 9.933 0.000 0.0 06 A6 4966391.000 13.162 13.162 0.000 0.0 07 A7 1062516.000 3.390 3.390 0.000 0.0 08 A8 2073709.000 5.921 5.921 0.000 0.0 09 A9 2401142.000 6.741 6.741 0.000 0.0 10 A10 3056521.000 8.381 8.381 0.000 0.0 11 A11 2246573.000 6.354 6.354 0.000 0.0 12 A12 2482193.000 6.944 6.944 0.000 0.0 13 B1 1540721.000 4.587 4.587 0.000 0.0 14 B2 1547113.000 4.603 4.603 0.000 0.0 15 B3 4904388.000 13.007 13.007 0.000 0.0 16 B4 1837121.000 5.329 5.329 0.000 0.0 17 B5 1669934.000 4.911 4.911 0.000 0.0 18 B6 2171953.000 6.167 6.167 0.000 0.0 19 B7 1228279.000 3.805 3.805 0.000 0.0 20 B8 2719207.000 7.537 7.537 0.000 0.0 21 B9 1647226.000 4.854 4.854 0.000 0.0 22 B10 2342815.000 6.595 6.595 0.000 0.0 23 B11 2528237.000 7.059 7.059 0.000 0.0 24 B12 2355542.000 6.627 6.627 0.000 0.0 25 C1 1837332.000 5.330 5.330 0.000 0.0 26 C2 1046809.000 3.351 3.351 0.000 0.0 27 C3 2469415.000 6.912 6.912 0.000 0.0 28 C4 2209713.000 6.262 6.262 0.000 0.0 29 C5 7674062.000 19.940 19.940 0.000 0.0 30 C6 1682566.000 4.942 4.942 0.000 0.0 31 C7 1666329.000 4.902 4.902 0.000 0.0 32 C8 3130523.000 8.567 8.567 0.000 0.0 33 C9 1619434.000 4.784 4.784 0.000 0.0 34 C10 9206457.000 23.776 23.776 0.000 0.0 35 C11 357020.000 1.624 1.624 0.000 0.0 36 C12 2251980.000 6.368 6.368 0.000 0.0 37 D1 1430379.000 4.311 4.311 0.000 0.0 38 D2 1527889.000 4.555 4.555 0.000 0.0 39 D3 3123768.000 8.550 8.550 0.000 0.0 40 D4 5774399.000 15.185 15.185 0.000 0.0 41 D5 3709865.000 10.017 10.017 0.000 0.0 42 D6 2242280.000 6.343 6.343 0.000 0.0 43 D7 2877483.000 7.933 7.933 0.000 0.0 44 D8 7218791.000 18.800 18.800 0.000 0.0 45 D9 5164358.000 13.658 13.658 0.000 0.0 46 D10 1537921.000 4.580 4.580 0.000 0.0 47 D11 1712656.000 5.018 5.018 0.000 0.0 48 D12 6264001.000 16.410 16.410 0.000 0.0 49 E1 740427.000 2.584 2.584 0.000 0.0 50 E2 1843261.000 5.345 5.345 0.000 0.0 51 E3 8489010.000 21.980 21.980 0.000 0.0 52 E4 3659930.000 9.892 9.892 0.000 0.0 53 E5 3648964.000 9.864 9.864 0.000 0.0 54 E6 4782629.000 12.702 12.702 0.000 0.0 55 E7 1660379.000 4.887 4.887 0.000 0.0 56 E8 10607101.000 27.282 27.282 0.000 0.0 57 E9 2228772.000 6.309 6.309 0.000 0.0 58 E10 1695028.000 4.973 4.973 0.000 0.0 59 E11 5687698.000 14.968 14.968 0.000 0.0 60 E12 1321.000 R 0.734 0.734 0.000 0.0 61 F1 2074248.000 5.923 5.923 0.000 0.0 62 F2 3293514.000 8.975 8.975 0.000 0.0 63 F3 2334241.000 6.574 6.574 0.000 0.0 64 F4 8224105.000 21.317 21.317 0.000 0.0 65 F5 3378719.000 9.188 9.188 0.000 0.0 66 F6 1097237.000 3.477 3.477 0.000 0.0 67 F7 2561387.000 7.142 7.142 0.000 0.0 68 F8 4701423.000 12.499 12.499 0.000 0.0 69 F9 1668952.000 4.908 4.908 0.000 0.0 70 F10 3082476.000 8.446 8.446 0.000 0.0 71 F11 10776274.000 27.705 27.705 0.000 0.0 72 F12 1401.000 R 0.734 0.734 0.000 0.0 73 G1 3819637.000 10.292 10.292 0.000 0.0 74 G2 3571870.000 9.671 9.671 0.000 0.0 75 G3 6661179.000 17.404 17.404 0.000 0.0 76 G4 2728792.000 7.561 7.561 0.000 0.0 77 G5 4818703.000 12.792 12.792 0.000 0.0 78 G6 1276543.000 3.926 3.926 0.000 0.0 79 G7 2015814.000 5.776 5.776 0.000 0.0 80 G8 6244621.000 16.362 16.362 0.000 0.0 81 G9 4011231.000 10.771 10.771 0.000 0.0 82 G10 2223427.000 6.296 6.296 0.000 0.0 83 G11 1346840.000 4.102 4.102 0.000 0.0 84 G12 1390.000 R 0.734 0.734 0.000 0.0 85 H1 2273985.000 6.423 6.423 0.000 0.0 86 H2 4103254.000 11.002 11.002 0.000 0.0 87 H3 7649281.000 19.878 19.878 0.000 0.0 88 H4 1630400.000 4.812 4.812 0.000 0.0 89 H5 3853221.000 10.376 10.376 0.000 0.0 90 H6 1029939.000 3.309 3.309 0.000 0.0 91 H7 793267.000 2.716 2.716 0.000 0.0 92 H8 3735288.000 10.081 10.081 0.000 0.0 93 H9 2179769.000 6.187 6.187 0.000 0.0 94 H10 1066528.000 3.400 3.400 0.000 0.0 95 H11 1904853.000 5.499 5.499 0.000 0.0 96 H12 1422.000 R 0.734 0.734 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: HiSeq_322_345_322_LibraryConc_01-12-2016; Date Last Saved: 1/12/2016 11:33:05 AM