##BLOCKS= 3 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 49017626 49354694 44426778 15527216 48425078 13215821 12650286 16930190 13767970 9151978 44633314 48772614 45517934 49085734 33909390 6368756 41871006 9950576 11088438 10624633 11155759 11744220 45180466 28886758 46459154 43605690 35814882 13434258 43158798 10353471 9000202 10401246 10825309 9779746 41439638 14120508 43302950 46596246 33488586 19617322 35130894 12084646 9718432 12674622 11270841 8400827 37765994 5482467 37400226 44771166 41555558 30369802 -151926 9396872 8666986 10635470 9970110 -151937 36583522 1793254 46931930 39006774 37305546 14377976 -151965 11406592 11243604 12279256 10345432 -151998 30210696 695541 49798254 40695638 42205870 47841330 -152021 11064154 11062999 3123755 10852199 -151983 -151945 264322 57400070 45862754 43095998 56751590 -152025 11780056 9246336 11755700 12515549 -151992 -151885 0 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 500.000 480.600 A12 48772614.000 48772614.000 0.000 0.0 02 250.000 285.195 B12 28886758.000 28886758.000 0.000 0.0 03 125.000 140.096 C12 14120508.000 14120508.000 0.000 0.0 04 62.500 55.215 D12 5482467.000 5482467.000 0.000 0.0 05 31.250 18.964 E12 1793254.000 1793254.000 0.000 0.0 06 15.625 8.177 F12 695541.000 695541.000 0.000 0.0 07 7.813 3.940 G12 264322.000 264322.000 0.000 0.0 Group Summaries MinStd Smallest standard value: 264322.000 Min(MeanValue) MaxStd Largest standard value: 48772614.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 49017626.000 R 483.008 483.008 0.000 0.0 02 A2 49354694.000 R 486.320 486.320 0.000 0.0 03 A3 44426778.000 437.897 437.897 0.000 0.0 04 A4 15527216.000 153.919 153.919 0.000 0.0 05 A5 48425078.000 477.185 477.185 0.000 0.0 06 A6 13215821.000 131.206 131.206 0.000 0.0 07 A7 12650286.000 125.649 125.649 0.000 0.0 08 A8 16930190.000 167.705 167.705 0.000 0.0 09 A9 13767970.000 136.632 136.632 0.000 0.0 10 A10 9151978.000 91.273 91.273 0.000 0.0 11 A11 44633314.000 439.926 439.926 0.000 0.0 12 B1 45517934.000 448.619 448.619 0.000 0.0 13 B2 49085734.000 R 483.677 483.677 0.000 0.0 14 B3 33909390.000 334.549 334.549 0.000 0.0 15 B4 6368756.000 63.924 63.924 0.000 0.0 16 B5 41871006.000 412.783 412.783 0.000 0.0 17 B6 9950576.000 99.121 99.121 0.000 0.0 18 B7 11088438.000 110.302 110.302 0.000 0.0 19 B8 10624633.000 105.744 105.744 0.000 0.0 20 B9 11155759.000 110.963 110.963 0.000 0.0 21 B10 11744220.000 116.746 116.746 0.000 0.0 22 B11 45180466.000 445.303 445.303 0.000 0.0 23 C1 46459154.000 457.867 457.867 0.000 0.0 24 C2 43605690.000 429.828 429.828 0.000 0.0 25 C3 35814882.000 353.273 353.273 0.000 0.0 26 C4 13434258.000 133.353 133.353 0.000 0.0 27 C5 43158798.000 425.437 425.437 0.000 0.0 28 C6 10353471.000 103.080 103.080 0.000 0.0 29 C7 9000202.000 89.782 89.782 0.000 0.0 30 C8 10401246.000 103.549 103.549 0.000 0.0 31 C9 10825309.000 107.716 107.716 0.000 0.0 32 C10 9779746.000 97.442 97.442 0.000 0.0 33 C11 41439638.000 408.544 408.544 0.000 0.0 34 D1 43302950.000 426.853 426.853 0.000 0.0 35 D2 46596246.000 459.215 459.215 0.000 0.0 36 D3 33488586.000 330.414 330.414 0.000 0.0 37 D4 19617322.000 194.110 194.110 0.000 0.0 38 D5 35130894.000 346.552 346.552 0.000 0.0 39 D6 12084646.000 120.091 120.091 0.000 0.0 40 D7 9718432.000 96.840 96.840 0.000 0.0 41 D8 12674622.000 125.888 125.888 0.000 0.0 42 D9 11270841.000 112.094 112.094 0.000 0.0 43 D10 8400827.000 83.892 83.892 0.000 0.0 44 D11 37765994.000 372.445 372.445 0.000 0.0 45 E1 37400226.000 368.851 368.851 0.000 0.0 46 E2 44771166.000 441.281 441.281 0.000 0.0 47 E3 41555558.000 409.683 409.683 0.000 0.0 48 E4 30369802.000 299.768 299.768 0.000 0.0 49 E5 -151926.000 R -0.150 -0.150 0.000 0.0 50 E6 9396872.000 93.680 93.680 0.000 0.0 51 E7 8666986.000 86.508 86.508 0.000 0.0 52 E8 10635470.000 105.851 105.851 0.000 0.0 53 E9 9970110.000 99.313 99.313 0.000 0.0 54 E10 -151937.000 R -0.150 -0.150 0.000 0.0 55 E11 36583522.000 360.826 360.826 0.000 0.0 56 F1 46931930.000 462.513 462.513 0.000 0.0 57 F2 39006774.000 384.638 384.638 0.000 0.0 58 F3 37305546.000 367.921 367.921 0.000 0.0 59 F4 14377976.000 142.626 142.626 0.000 0.0 60 F5 -151965.000 R -0.151 -0.151 0.000 0.0 61 F6 11406592.000 113.428 113.428 0.000 0.0 62 F7 11243604.000 111.826 111.826 0.000 0.0 63 F8 12279256.000 122.003 122.003 0.000 0.0 64 F9 10345432.000 103.001 103.001 0.000 0.0 65 F10 -151998.000 R -0.151 -0.151 0.000 0.0 66 F11 30210696.000 298.204 298.204 0.000 0.0 67 G1 49798254.000 R 490.679 490.679 0.000 0.0 68 G2 40695638.000 401.233 401.233 0.000 0.0 69 G3 42205870.000 416.073 416.073 0.000 0.0 70 G4 47841330.000 471.449 471.449 0.000 0.0 71 G5 -152021.000 R -0.151 -0.151 0.000 0.0 72 G6 11064154.000 110.063 110.063 0.000 0.0 73 G7 11062999.000 110.052 110.052 0.000 0.0 74 G8 3123755.000 32.038 32.038 0.000 0.0 75 G9 10852199.000 107.980 107.980 0.000 0.0 76 G10 -151983.000 R -0.151 -0.151 0.000 0.0 77 G11 -151945.000 R -0.150 -0.150 0.000 0.0 78 H1 57400070.000 R 565.377 565.377 0.000 0.0 79 H2 45862754.000 452.007 452.007 0.000 0.0 80 H3 43095998.000 424.820 424.820 0.000 0.0 81 H4 56751590.000 R 559.005 559.005 0.000 0.0 82 H5 -152025.000 R -0.151 -0.151 0.000 0.0 83 H6 11780056.000 117.098 117.098 0.000 0.0 84 H7 9246336.000 92.201 92.201 0.000 0.0 85 H8 11755700.000 116.859 116.859 0.000 0.0 86 H9 12515549.000 124.325 124.325 0.000 0.0 87 H10 -151992.000 R -0.151 -0.151 0.000 0.0 88 H11 -151885.000 R -0.150 -0.150 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: HiSeq_424_410_397_TotalRNAConc_02-29-2016; Date Last Saved: 2/29/2016 2:09:45 PM