##BLOCKS= 5 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 -23512 -23484 -23204 -23386 -23147 -23419 -22752 -23177 -22599 21498191 -23652 -23669 -23320 -23295 -23295 -23423 -23264 -23072 -22718 -23108 -23264 10402532 -23598 -23680 -23333 -23451 -23298 -23216 -23304 -22893 -23183 -23189 -22937 5090639 -23641 -23592 -23392 -23315 -23080 -23498 -23016 -23194 -23041 -23087 -23256 2164231 -23549 -23696 -23383 -23233 -23269 -23343 -23283 -23158 -23199 -23207 -23245 1059303 -23590 -23590 -23363 -23229 -23174 -23240 -23111 -23253 -23108 -23242 -23348 515795 -23609 -23590 -23281 -23120 -23276 -23342 -23274 -23323 -23209 -23176 -23261 252000 -23566 -23683 -23147 -23196 -23288 -23396 -23396 -23230 -23222 -23208 -23673 0 -23469 -23534 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 2937661 1505209 4051026 30075 24526 32387 8162293 5372630 8251123 21360942 1114 1144 3969998 1648976 2038505 28550 31011 35837 2606802 2256753 3091706 10655935 1054 986 2632048 886045 1981645 26739 35975 32646 14733238 9023055 12406664 5259597 1254 1240 3097037 2646539 3288330 25772 30573 32917 6515164 241544 9189756 2221933 1180 1158 2831089 3876055 2202524 26983 34857 30727 2317498 3240001 10630264 1088446 1156 1215 1998899 3075982 3117793 25605 32160 33708 6252309 4046137 14017434 548169 1177 1118 2525497 2976372 815463 24610 33171 32146 6814341 2736245 6221729 278425 1155 1155 1311779 3063373 1643792 30226 36414 32258 13021085 5989464 29803 20057 1255 1252 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 50.000 50.248 A10 21498191.000 21498191.000 0.000 0.0 02 25.000 24.662 B10 10402532.000 10402532.000 0.000 0.0 03 12.500 12.412 C10 5090639.000 5090639.000 0.000 0.0 04 6.250 5.664 D10 2164231.000 2164231.000 0.000 0.0 05 3.125 3.116 E10 1059303.000 1059303.000 0.000 0.0 06 1.563 1.863 F10 515795.000 515795.000 0.000 0.0 07 0.781 1.254 G10 252000.000 252000.000 0.000 0.0 Group Summaries MinStd Smallest standard value: 252000.000 Min(MeanValue) MaxStd Largest standard value: 21498191.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 2937661.000 7.447 7.447 0.000 0.0 02 A2 1505209.000 4.144 4.144 0.000 0.0 03 A3 4051026.000 10.015 10.015 0.000 0.0 04 A4 30075.000 R 0.743 0.743 0.000 0.0 05 A5 24526.000 R 0.730 0.730 0.000 0.0 06 A6 32387.000 R 0.748 0.748 0.000 0.0 07 A7 8162293.000 19.496 19.496 0.000 0.0 08 A8 5372630.000 13.063 13.063 0.000 0.0 09 A9 8251123.000 19.700 19.700 0.000 0.0 10 B1 3969998.000 9.828 9.828 0.000 0.0 11 B2 1648976.000 4.476 4.476 0.000 0.0 12 B3 2038505.000 5.374 5.374 0.000 0.0 13 B4 28550.000 R 0.739 0.739 0.000 0.0 14 B5 31011.000 R 0.745 0.745 0.000 0.0 15 B6 35837.000 R 0.756 0.756 0.000 0.0 16 B7 2606802.000 6.684 6.684 0.000 0.0 17 B8 2256753.000 5.877 5.877 0.000 0.0 18 B9 3091706.000 7.803 7.803 0.000 0.0 19 C1 2632048.000 6.743 6.743 0.000 0.0 20 C2 886045.000 2.716 2.716 0.000 0.0 21 C3 1981645.000 5.243 5.243 0.000 0.0 22 C4 26739.000 R 0.735 0.735 0.000 0.0 23 C5 35975.000 R 0.756 0.756 0.000 0.0 24 C6 32646.000 R 0.748 0.748 0.000 0.0 25 C7 14733238.000 34.648 34.648 0.000 0.0 26 C8 9023055.000 21.480 21.480 0.000 0.0 27 C9 12406664.000 29.283 29.283 0.000 0.0 28 D1 3097037.000 7.815 7.815 0.000 0.0 29 D2 2646539.000 6.776 6.776 0.000 0.0 30 D3 3288330.000 8.256 8.256 0.000 0.0 31 D4 25772.000 R 0.733 0.733 0.000 0.0 32 D5 30573.000 R 0.744 0.744 0.000 0.0 33 D6 32917.000 R 0.749 0.749 0.000 0.0 34 D7 6515164.000 15.697 15.697 0.000 0.0 35 D8 241544.000 R 1.230 1.230 0.000 0.0 36 D9 9189756.000 21.865 21.865 0.000 0.0 37 E1 2831089.000 7.202 7.202 0.000 0.0 38 E2 3876055.000 9.611 9.611 0.000 0.0 39 E3 2202524.000 5.752 5.752 0.000 0.0 40 E4 26983.000 R 0.735 0.735 0.000 0.0 41 E5 34857.000 R 0.754 0.754 0.000 0.0 42 E6 30727.000 R 0.744 0.744 0.000 0.0 43 E7 2317498.000 6.017 6.017 0.000 0.0 44 E8 3240001.000 8.145 8.145 0.000 0.0 45 E9 10630264.000 25.187 25.187 0.000 0.0 46 F1 1998899.000 5.283 5.283 0.000 0.0 47 F2 3075982.000 7.766 7.766 0.000 0.0 48 F3 3117793.000 7.863 7.863 0.000 0.0 49 F4 25605.000 R 0.732 0.732 0.000 0.0 50 F5 32160.000 R 0.747 0.747 0.000 0.0 51 F6 33708.000 R 0.751 0.751 0.000 0.0 52 F7 6252309.000 15.091 15.091 0.000 0.0 53 F8 4046137.000 10.004 10.004 0.000 0.0 54 F9 14017434.000 32.998 32.998 0.000 0.0 55 G1 2525497.000 6.497 6.497 0.000 0.0 56 G2 2976372.000 7.537 7.537 0.000 0.0 57 G3 815463.000 2.554 2.554 0.000 0.0 58 G4 24610.000 R 0.730 0.730 0.000 0.0 59 G5 33171.000 R 0.750 0.750 0.000 0.0 60 G6 32146.000 R 0.747 0.747 0.000 0.0 61 G7 6814341.000 16.387 16.387 0.000 0.0 62 G8 2736245.000 6.983 6.983 0.000 0.0 63 G9 6221729.000 15.021 15.021 0.000 0.0 64 H1 1311779.000 3.698 3.698 0.000 0.0 65 H2 3063373.000 7.737 7.737 0.000 0.0 66 H3 1643792.000 4.464 4.464 0.000 0.0 67 H4 30226.000 R 0.743 0.743 0.000 0.0 68 H5 36414.000 R 0.757 0.757 0.000 0.0 69 H6 32258.000 R 0.748 0.748 0.000 0.0 70 H7 13021085.000 30.700 30.700 0.000 0.0 71 H8 5989464.000 14.485 14.485 0.000 0.0 72 H9 29803.000 R 0.742 0.742 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: LibraryConcForSiranoosh_12-16-2015; Date Last Saved: 12/16/2015 12:09:56 PM