##BLOCKS= 4 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 2766523 1414380 3845520 9868 4153 11328 7697097 5086739 7748699 20158994 -16021 -16026 3647470 1520528 1897125 9073 10369 15594 2442017 2110835 2911201 10051237 -16059 -16102 2414066 809150 1853481 7865 12989 11570 13899230 8428239 11682373 5025230 -15917 -15939 2897371 2461626 3062906 6778 10689 12042 6150967 211250 8718344 2129529 -15963 -16044 2629758 3632115 2086098 8220 12930 9388 2200777 3054219 10044674 1035488 -15798 -16060 1856754 2860822 2955378 7076 10297 11828 5949980 3831432 13224364 507118 -15869 -15931 2373225 2745267 762450 3753 11529 10400 6531267 2599794 5886219 252451 -15880 -16022 1215723 2839263 1550163 11514 15112 10787 12431309 5665652 8123 0 -15934 -15953 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 50.000 49.945 A10 20158994.000 20158994.000 0.000 0.0 02 25.000 25.120 B10 10051237.000 10051237.000 0.000 0.0 03 12.500 12.776 C10 5025230.000 5025230.000 0.000 0.0 04 6.250 5.665 D10 2129529.000 2129529.000 0.000 0.0 05 3.125 2.978 E10 1035488.000 1035488.000 0.000 0.0 06 1.563 1.680 F10 507118.000 507118.000 0.000 0.0 07 0.781 1.055 G10 252451.000 252451.000 0.000 0.0 Group Summaries MinStd Smallest standard value: 252451.000 Min(MeanValue) MaxStd Largest standard value: 20158994.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 2766523.000 7.229 7.229 0.000 0.0 02 A2 1414380.000 3.908 3.908 0.000 0.0 03 A3 3845520.000 9.879 9.879 0.000 0.0 04 A4 9868.000 R 0.459 0.459 0.000 0.0 05 A5 4153.000 R 0.445 0.445 0.000 0.0 06 A6 11328.000 R 0.462 0.462 0.000 0.0 07 A7 7697097.000 19.339 19.339 0.000 0.0 08 A8 5086739.000 12.928 12.928 0.000 0.0 09 A9 7748699.000 19.465 19.465 0.000 0.0 10 B1 3647470.000 9.393 9.393 0.000 0.0 11 B2 1520528.000 4.169 4.169 0.000 0.0 12 B3 1897125.000 5.094 5.094 0.000 0.0 13 B4 9073.000 R 0.457 0.457 0.000 0.0 14 B5 10369.000 R 0.460 0.460 0.000 0.0 15 B6 15594.000 R 0.473 0.473 0.000 0.0 16 B7 2442017.000 6.432 6.432 0.000 0.0 17 B8 2110835.000 5.619 5.619 0.000 0.0 18 B9 2911201.000 7.584 7.584 0.000 0.0 19 C1 2414066.000 6.363 6.363 0.000 0.0 20 C2 809150.000 2.422 2.422 0.000 0.0 21 C3 1853481.000 4.987 4.987 0.000 0.0 22 C4 7865.000 R 0.454 0.454 0.000 0.0 23 C5 12989.000 R 0.466 0.466 0.000 0.0 24 C6 11570.000 R 0.463 0.463 0.000 0.0 25 C7 13899230.000 34.571 34.571 0.000 0.0 26 C8 8428239.000 21.134 21.134 0.000 0.0 27 C9 11682373.000 29.126 29.126 0.000 0.0 28 D1 2897371.000 7.550 7.550 0.000 0.0 29 D2 2461626.000 6.480 6.480 0.000 0.0 30 D3 3062906.000 7.957 7.957 0.000 0.0 31 D4 6778.000 R 0.451 0.451 0.000 0.0 32 D5 10689.000 R 0.461 0.461 0.000 0.0 33 D6 12042.000 R 0.464 0.464 0.000 0.0 34 D7 6150967.000 15.541 15.541 0.000 0.0 35 D8 211250.000 R 0.953 0.953 0.000 0.0 36 D9 8718344.000 21.847 21.847 0.000 0.0 37 E1 2629758.000 6.893 6.893 0.000 0.0 38 E2 3632115.000 9.355 9.355 0.000 0.0 39 E3 2086098.000 5.558 5.558 0.000 0.0 40 E4 8220.000 R 0.455 0.455 0.000 0.0 41 E5 12930.000 R 0.466 0.466 0.000 0.0 42 E6 9388.000 R 0.458 0.458 0.000 0.0 43 E7 2200777.000 5.840 5.840 0.000 0.0 44 E8 3054219.000 7.936 7.936 0.000 0.0 45 E9 10044674.000 25.104 25.104 0.000 0.0 46 F1 1856754.000 4.995 4.995 0.000 0.0 47 F2 2860822.000 7.461 7.461 0.000 0.0 48 F3 2955378.000 7.693 7.693 0.000 0.0 49 F4 7076.000 R 0.452 0.452 0.000 0.0 50 F5 10297.000 R 0.460 0.460 0.000 0.0 51 F6 11828.000 R 0.464 0.464 0.000 0.0 52 F7 5949980.000 15.048 15.048 0.000 0.0 53 F8 3831432.000 9.845 9.845 0.000 0.0 54 F9 13224364.000 32.914 32.914 0.000 0.0 55 G1 2373225.000 6.263 6.263 0.000 0.0 56 G2 2745267.000 7.177 7.177 0.000 0.0 57 G3 762450.000 2.307 2.307 0.000 0.0 58 G4 3753.000 R 0.444 0.444 0.000 0.0 59 G5 11529.000 R 0.463 0.463 0.000 0.0 60 G6 10400.000 R 0.460 0.460 0.000 0.0 61 G7 6531267.000 16.475 16.475 0.000 0.0 62 G8 2599794.000 6.820 6.820 0.000 0.0 63 G9 5886219.000 14.891 14.891 0.000 0.0 64 H1 1215723.000 3.420 3.420 0.000 0.0 65 H2 2839263.000 7.408 7.408 0.000 0.0 66 H3 1550163.000 4.242 4.242 0.000 0.0 67 H4 11514.000 R 0.463 0.463 0.000 0.0 68 H5 15112.000 R 0.472 0.472 0.000 0.0 69 H6 10787.000 R 0.461 0.461 0.000 0.0 70 H7 12431309.000 30.966 30.966 0.000 0.0 71 H8 5665652.000 14.349 14.349 0.000 0.0 72 H9 8123.000 R 0.454 0.454 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: LibraryConcForSiranoosh_2ndRead_12-16-2015; Date Last Saved: 12/16/2015 12:15:03 PM