##BLOCKS= 4 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 10022276 2858483 8179056 -20020 -20048 -20017 -19978 -19981 -19919 -20015 -20004 22565836 14143910 4186170 1993938 -19954 -19948 -19900 -19849 -19900 -19919 -19936 -20029 10634471 12201964 5905341 2657713 -20025 -19969 -19910 -19896 -19955 -19947 -19880 -19998 5132836 16310540 2894191 9806859 -19943 -19861 -19926 -19889 -19965 -20027 -19985 -19948 2262130 21351918 9560135 2947411 -19936 -19945 -19920 -19934 -19852 -19988 -19909 -19954 1097333 14903683 8620224 1088088 -19928 -19911 -19942 -19919 -19944 -19910 -19983 -19972 543782 22967570 10788439 28345546 -19997 -20016 -19916 -20008 -19994 -16603 -19902 -19972 248188 21785958 13754921 7187000 -19999 -20002 -19935 -19960 -19920 -19957 -19976 -19979 0 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 50.000 50.511 A12 22565836.000 22565836.000 0.000 0.0 02 25.000 24.230 B12 10634471.000 10634471.000 0.000 0.0 03 12.500 12.112 C12 5132836.000 5132836.000 0.000 0.0 04 6.250 5.788 D12 2262130.000 2262130.000 0.000 0.0 05 3.125 3.223 E12 1097333.000 1097333.000 0.000 0.0 06 1.563 2.003 F12 543782.000 543782.000 0.000 0.0 07 0.781 1.352 G12 248188.000 248188.000 0.000 0.0 Group Summaries MinStd Smallest standard value: 248188.000 Min(MeanValue) MaxStd Largest standard value: 22565836.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 10022276.000 22.881 22.881 0.000 0.0 02 B1 14143910.000 31.960 31.960 0.000 0.0 03 C1 12201964.000 27.683 27.683 0.000 0.0 04 D1 16310540.000 36.733 36.733 0.000 0.0 05 E1 21351918.000 47.837 47.837 0.000 0.0 06 F1 14903683.000 33.634 33.634 0.000 0.0 07 G1 22967570.000 R 51.396 51.396 0.000 0.0 08 H1 21785958.000 48.793 48.793 0.000 0.0 09 A2 2858483.000 7.102 7.102 0.000 0.0 10 B2 4186170.000 10.026 10.026 0.000 0.0 11 C2 5905341.000 13.813 13.813 0.000 0.0 12 D2 2894191.000 7.180 7.180 0.000 0.0 13 E2 9560135.000 21.864 21.864 0.000 0.0 14 F2 8620224.000 19.793 19.793 0.000 0.0 15 G2 10788439.000 24.569 24.569 0.000 0.0 16 H2 13754921.000 31.103 31.103 0.000 0.0 17 A3 8179056.000 18.821 18.821 0.000 0.0 18 B3 1993938.000 5.198 5.198 0.000 0.0 19 C3 2657713.000 6.660 6.660 0.000 0.0 20 D3 9806859.000 22.407 22.407 0.000 0.0 21 E3 2947411.000 7.298 7.298 0.000 0.0 22 F3 1088088.000 3.202 3.202 0.000 0.0 23 G3 28345546.000 R 63.242 63.242 0.000 0.0 24 H3 7187000.000 16.636 16.636 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: UCD001_LibConc_12-16-2015; Date Last Saved: 12/16/2015 11:15:55 AM