##BLOCKS= 4 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 23.5 7666 1422420 1946862 1792786 772.5 641889.5 562805.5 -5211.5 -5225.5 -5171.5 -5030.5 -5168.5 7839 1320685 1775156 1005388 98.5 683852.5 814552.5 -5277.5 -5215.5 -5057.5 -5084.5 -5122.5 7305 1273943 1610062 815959 -156.5 1104429.5 836904.5 -5331.5 -5315.5 -5245.5 -5128.5 -5134.5 7776 635918 1120778 1043975 -277.5 597578.5 627720.5 -5387.5 -5384.5 -5148.5 -5216.5 -5083.5 7835 501127 618392 704778 -132.5 351806.5 696505.5 -5188.5 -5212.5 -5321.5 -5177.5 -4983.5 8652 548043 432283 328555 -393.5 434532.5 770975.5 -5327.5 -5427.5 -3963.5 -5132.5 -5108.5 7580 338921 437394 305361 -138.5 530398.5 799994.5 -5252.5 -5333.5 -5239.5 -5167.5 -5110.5 11467 147390 164894 201518 227.5 461526.5 328636.5 -5354.5 -5211.5 -5273.5 -5149.5 -5121.5 1 2 3 4 5 6 7 8 9 10 11 12 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV Group Summaries MinStd Smallest standard value: Min(MeanValue) MaxStd Largest standard value: Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Error Group Summaries InRange R - Outside standard range ~End Original Filename: 2016-05-24_Plate2; Date Last Saved: 5/24/2016 2:51:40 PM