##BLOCKS= 5 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 41234 3328655 3512809 4318025 9092 160411 75207 151429 131983 82351 338247 230784 9231 4041258 2797941 3320859 10367 158703 81645 442112 119123 145117 336319 155016 2130 3063454 2301388 2478633 9096 199751 97325 65298 134007 161437 410034 140642 2208 2237888 1829981 1819488 8746 547874 88549 74336 140646 118377 202470 213418 2014 1309394 1282656 1044303 8670 173806 102834 72000 125259 76322 36084 141451 2270 802209 809775 863806 43050 178543 113814 55581 74564 135559 271339 198431 2357 507356 538013 553156 8850 182360 130146 68622 108966 110861 285287 103902 2370 333275 280949 338355 8488 130525 198026 69420 128380 100833 212780 128302 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 85829 289958 230431 163747 439886 357368 435976 336130 225572 159570 308224 152969 70329 210846 327809 117351 551069 479541 32289 259250 207003 196861 323878 12198 57110 230629 249149 57966 541060 327759 25184 368284 208894 128094 419307 35247 94055 229207 271668 84066 493426 372842 403257 300383 288035 239470 319887 48949 175117 283593 243584 102566 542238 444613 397551 324885 367194 272516 309229 163910 68321 206804 236049 81103 519280 351722 305305 289161 408352 280514 281497 251695 161145 297560 10184 78983 441403 362769 36215 295301 414509 360547 369583 219500 143347 292564 9548 88680 406836 215575 41339 280662 383024 257056 290777 12417 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 100.000 72.733 A2 3328655.000 3719829.667 526171.471 14.1 77.865 A3 3512809.000 100.307 A4 4318025.000 02 60.000 92.594 B2 4041258.000 3386686.000 624266.919 18.4 57.942 B3 2797941.000 72.516 B4 3320859.000 03 36.000 65.342 C2 3063454.000 2614491.667 398784.817 15.3 44.103 C3 2301388.000 49.042 C4 2478633.000 04 21.600 42.333 D2 2237888.000 1962452.333 238591.975 12.2 30.964 D3 1829981.000 30.672 D4 1819488.000 05 12.960 16.455 E2 1309394.000 1212117.667 145945.373 12.0 15.710 E3 1282656.000 9.067 E4 1044303.000 06 7.776 2.320 F2 802209.000 825263.333 33592.618 4.1 2.531 F3 809775.000 4.037 F4 863806.000 07 4.666 -5.898 G2 507356.000 532841.667 23333.817 4.4 -5.044 G3 538013.000 -4.621 G4 553156.000 08 2.799 -10.750 H2 333275.000 317526.333 31778.571 10.0 -12.208 H3 280949.000 -10.608 H4 338355.000 Group Summaries MinStd Smallest standard value: 317526.333 Min(MeanValue) MaxStd Largest standard value: 3719829.667 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A5 9092.000 R -19.785 -19.785 0.000 0.0 02 B5 10367.000 R -19.749 -19.749 0.000 0.0 03 C5 9096.000 R -19.785 -19.785 0.000 0.0 04 D5 8746.000 R -19.794 -19.794 0.000 0.0 05 E5 8670.000 R -19.797 -19.797 0.000 0.0 06 F5 43050.000 R -18.838 -18.838 0.000 0.0 065 A1 85829.000 R -17.646 -17.646 0.000 0.0 066 B1 70329.000 R -18.078 -18.078 0.000 0.0 067 C1 57110.000 R -18.446 -18.446 0.000 0.0 068 D1 94055.000 R -17.417 -17.417 0.000 0.0 069 E1 175117.000 R -15.158 -15.158 0.000 0.0 07 G5 8850.000 R -19.792 -19.792 0.000 0.0 070 F1 68321.000 R -18.134 -18.134 0.000 0.0 071 G1 161145.000 R -15.547 -15.547 0.000 0.0 072 H1 143347.000 R -16.043 -16.043 0.000 0.0 073 A2 289958.000 R -11.957 -11.957 0.000 0.0 074 B2 210846.000 R -14.162 -14.162 0.000 0.0 075 C2 230629.000 R -13.610 -13.610 0.000 0.0 076 D2 229207.000 R -13.650 -13.650 0.000 0.0 077 E2 283593.000 R -12.134 -12.134 0.000 0.0 078 F2 206804.000 R -14.274 -14.274 0.000 0.0 079 G2 297560.000 R -11.745 -11.745 0.000 0.0 08 H5 8488.000 R -19.802 -19.802 0.000 0.0 080 H2 292564.000 R -11.884 -11.884 0.000 0.0 081 A3 230431.000 R -13.616 -13.616 0.000 0.0 082 B3 327809.000 -10.902 -10.902 0.000 0.0 083 C3 249149.000 R -13.094 -13.094 0.000 0.0 084 D3 271668.000 R -12.467 -12.467 0.000 0.0 085 E3 243584.000 R -13.249 -13.249 0.000 0.0 086 F3 236049.000 R -13.459 -13.459 0.000 0.0 087 G3 10184.000 R -19.754 -19.754 0.000 0.0 088 H3 9548.000 R -19.772 -19.772 0.000 0.0 089 A4 163747.000 R -15.474 -15.474 0.000 0.0 09 A6 160411.000 R -15.567 -15.567 0.000 0.0 090 B4 117351.000 R -16.768 -16.768 0.000 0.0 091 C4 57966.000 R -18.423 -18.423 0.000 0.0 092 D4 84066.000 R -17.695 -17.695 0.000 0.0 093 E4 102566.000 R -17.180 -17.180 0.000 0.0 094 F4 81103.000 R -17.778 -17.778 0.000 0.0 095 G4 78983.000 R -17.837 -17.837 0.000 0.0 096 H4 88680.000 R -17.567 -17.567 0.000 0.0 097 A5 439886.000 -7.778 -7.778 0.000 0.0 098 B5 551069.000 -4.680 -4.680 0.000 0.0 099 C5 541060.000 -4.959 -4.959 0.000 0.0 10 B6 158703.000 R -15.615 -15.615 0.000 0.0 100 D5 493426.000 -6.286 -6.286 0.000 0.0 101 E5 542238.000 -4.926 -4.926 0.000 0.0 102 F5 519280.000 -5.566 -5.566 0.000 0.0 103 G5 441403.000 -7.736 -7.736 0.000 0.0 104 H5 406836.000 -8.699 -8.699 0.000 0.0 105 A6 357368.000 -10.078 -10.078 0.000 0.0 106 B6 479541.000 -6.673 -6.673 0.000 0.0 107 C6 327759.000 -10.903 -10.903 0.000 0.0 108 D6 372842.000 -9.647 -9.647 0.000 0.0 109 E6 444613.000 -7.647 -7.647 0.000 0.0 11 C6 199751.000 R -14.471 -14.471 0.000 0.0 110 F6 351722.000 -10.236 -10.236 0.000 0.0 111 G6 362769.000 -9.928 -9.928 0.000 0.0 112 H6 215575.000 R -14.030 -14.030 0.000 0.0 113 A7 435976.000 -7.887 -7.887 0.000 0.0 114 B7 32289.000 R -19.138 -19.138 0.000 0.0 115 C7 25184.000 R -19.336 -19.336 0.000 0.0 116 D7 403257.000 -8.799 -8.799 0.000 0.0 117 E7 397551.000 -8.958 -8.958 0.000 0.0 118 F7 305305.000 R -11.529 -11.529 0.000 0.0 119 G7 36215.000 R -19.029 -19.029 0.000 0.0 12 D6 547874.000 -4.769 -4.769 0.000 0.0 120 H7 41339.000 R -18.886 -18.886 0.000 0.0 121 A8 336130.000 -10.670 -10.670 0.000 0.0 122 B8 259250.000 R -12.813 -12.813 0.000 0.0 123 C8 368284.000 -9.774 -9.774 0.000 0.0 124 D8 300383.000 R -11.666 -11.666 0.000 0.0 125 E8 324885.000 -10.983 -10.983 0.000 0.0 126 F8 289161.000 R -11.979 -11.979 0.000 0.0 127 G8 295301.000 R -11.808 -11.808 0.000 0.0 128 H8 280662.000 R -12.216 -12.216 0.000 0.0 129 A9 225572.000 R -13.751 -13.751 0.000 0.0 13 E6 173806.000 R -15.194 -15.194 0.000 0.0 130 B9 207003.000 R -14.269 -14.269 0.000 0.0 131 C9 208894.000 R -14.216 -14.216 0.000 0.0 132 D9 288035.000 R -12.011 -12.011 0.000 0.0 133 E9 367194.000 -9.804 -9.804 0.000 0.0 134 F9 408352.000 -8.657 -8.657 0.000 0.0 135 G9 414509.000 -8.486 -8.486 0.000 0.0 136 H9 383024.000 -9.363 -9.363 0.000 0.0 137 A10 159570.000 R -15.591 -15.591 0.000 0.0 138 B10 196861.000 R -14.552 -14.552 0.000 0.0 139 C10 128094.000 R -16.468 -16.468 0.000 0.0 14 F6 178543.000 R -15.062 -15.062 0.000 0.0 140 D10 239470.000 R -13.364 -13.364 0.000 0.0 141 E10 272516.000 R -12.443 -12.443 0.000 0.0 142 F10 280514.000 R -12.220 -12.220 0.000 0.0 143 G10 360547.000 -9.990 -9.990 0.000 0.0 144 H10 257056.000 R -12.874 -12.874 0.000 0.0 145 A11 308224.000 R -11.448 -11.448 0.000 0.0 146 B11 323878.000 -11.012 -11.012 0.000 0.0 147 C11 419307.000 -8.352 -8.352 0.000 0.0 148 D11 319887.000 -11.123 -11.123 0.000 0.0 149 E11 309229.000 R -11.420 -11.420 0.000 0.0 15 G6 182360.000 R -14.956 -14.956 0.000 0.0 150 F11 281497.000 R -12.193 -12.193 0.000 0.0 151 G11 369583.000 -9.738 -9.738 0.000 0.0 152 H11 290777.000 R -11.934 -11.934 0.000 0.0 153 A12 152969.000 R -15.775 -15.775 0.000 0.0 154 B12 12198.000 R -19.698 -19.698 0.000 0.0 155 C12 35247.000 R -19.056 -19.056 0.000 0.0 156 D12 48949.000 R -18.674 -18.674 0.000 0.0 157 E12 163910.000 R -15.470 -15.470 0.000 0.0 158 F12 251695.000 R -13.023 -13.023 0.000 0.0 159 G12 219500.000 R -13.921 -13.921 0.000 0.0 16 H6 130525.000 R -16.400 -16.400 0.000 0.0 160 H12 12417.000 R -19.692 -19.692 0.000 0.0 17 A7 75207.000 R -17.942 -17.942 0.000 0.0 18 B7 81645.000 R -17.763 -17.763 0.000 0.0 19 C7 97325.000 R -17.326 -17.326 0.000 0.0 20 D7 88549.000 R -17.570 -17.570 0.000 0.0 21 E7 102834.000 R -17.172 -17.172 0.000 0.0 22 F7 113814.000 R -16.866 -16.866 0.000 0.0 23 G7 130146.000 R -16.411 -16.411 0.000 0.0 24 H7 198026.000 R -14.519 -14.519 0.000 0.0 25 A8 151429.000 R -15.818 -15.818 0.000 0.0 26 B8 442112.000 -7.716 -7.716 0.000 0.0 27 C8 65298.000 R -18.218 -18.218 0.000 0.0 28 D8 74336.000 R -17.966 -17.966 0.000 0.0 29 E8 72000.000 R -18.032 -18.032 0.000 0.0 30 F8 55581.000 R -18.489 -18.489 0.000 0.0 31 G8 68622.000 R -18.126 -18.126 0.000 0.0 32 H8 69420.000 R -18.103 -18.103 0.000 0.0 33 A9 131983.000 R -16.360 -16.360 0.000 0.0 34 B9 119123.000 R -16.718 -16.718 0.000 0.0 35 C9 134007.000 R -16.303 -16.303 0.000 0.0 36 D9 140646.000 R -16.118 -16.118 0.000 0.0 37 E9 125259.000 R -16.547 -16.547 0.000 0.0 38 F9 74564.000 R -17.960 -17.960 0.000 0.0 39 G9 108966.000 R -17.001 -17.001 0.000 0.0 40 H9 128380.000 R -16.460 -16.460 0.000 0.0 41 A10 82351.000 R -17.743 -17.743 0.000 0.0 42 B10 145117.000 R -15.994 -15.994 0.000 0.0 43 C10 161437.000 R -15.539 -15.539 0.000 0.0 44 D10 118377.000 R -16.739 -16.739 0.000 0.0 45 E10 76322.000 R -17.911 -17.911 0.000 0.0 46 F10 135559.000 R -16.260 -16.260 0.000 0.0 47 G10 110861.000 R -16.948 -16.948 0.000 0.0 48 H10 100833.000 R -17.228 -17.228 0.000 0.0 49 A11 338247.000 -10.611 -10.611 0.000 0.0 50 B11 336319.000 -10.665 -10.665 0.000 0.0 51 C11 410034.000 -8.610 -8.610 0.000 0.0 52 D11 202470.000 R -14.395 -14.395 0.000 0.0 53 E11 36084.000 R -19.032 -19.032 0.000 0.0 54 F11 271339.000 R -12.476 -12.476 0.000 0.0 55 G11 285287.000 R -12.087 -12.087 0.000 0.0 56 H11 212780.000 R -14.108 -14.108 0.000 0.0 57 A12 230784.000 R -13.606 -13.606 0.000 0.0 58 B12 155016.000 R -15.718 -15.718 0.000 0.0 59 C12 140642.000 R -16.118 -16.118 0.000 0.0 60 D12 213418.000 R -14.090 -14.090 0.000 0.0 61 E12 141451.000 R -16.096 -16.096 0.000 0.0 62 F12 198431.000 R -14.508 -14.508 0.000 0.0 63 G12 103902.000 R -17.142 -17.142 0.000 0.0 64 H12 128302.000 R -16.462 -16.462 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: 2015-12-18_BothPlates; Date Last Saved: 12/18/2015 10:29:42 AM