##BLOCKS= 5 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 35115 148158 100137 72884 226668 124793 239123 192024 124743 114306 177539 79816 29150 105152 171601 51281 207420 222859 15010 155730 103515 127240 186209 9682 25332 105903 108080 27330 208904 106331 13445 198889 97582 58788 223309 21971 40607 117425 134217 38759 270142 180182 182479 168811 160252 126725 140914 26356 74821 154497 126953 48302 271515 211539 157336 163305 205605 159498 174670 88281 28800 106028 121310 37993 268164 159399 128257 135714 240909 136951 156658 150002 77213 175092 9633 39287 248799 118416 15269 156495 233480 233555 227490 168213 65674 127627 7426 45981 219669 86040 16263 157409 220821 127079 180410 10727 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 1114 22647778 24512086 25676426 10932 49940 27602 54158 39733 21552 98638 87945 1150 20568052 24266164 23984500 10488 71521 30476 29640 45334 62917 100175 55231 1209 21992480 24841326 25580228 10659 106301 35488 22418 57562 56205 101168 65901 1188 21749368 15721731 21981776 10140 200724 33521 26135 33710 37935 96188 53447 1095 10416031 9878424 18796266 10381 82577 32354 25336 51021 19811 13114 56143 1120 3230513 3148396 3655964 10699 86623 28980 22848 25266 46991 73336 71956 1096 1415793 1285590 1515742 10673 92464 45206 28620 31741 35481 102429 38745 1176 676322 627264 834408 10487 65462 75799 33353 41273 43083 95125 56999 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 100.000 67.996 A2 22647778.000 24278763.333 1527745.652 6.3 75.769 A3 24512086.000 80.623 A4 25676426.000 02 60.000 59.325 B2 20568052.000 22939572.000 2058619.437 9.0 74.744 B3 24266164.000 73.569 B4 23984500.000 03 36.000 65.264 C2 21992480.000 24138011.333 1894458.384 7.8 77.142 C3 24841326.000 80.222 C4 25580228.000 04 21.600 64.250 D2 21749368.000 19817625.000 3549051.157 17.9 39.119 D3 15721731.000 65.219 D4 21981776.000 05 12.960 16.998 E2 10416031.000 13030240.333 5000754.374 38.4 14.757 E3 9878424.000 51.938 E4 18796266.000 06 7.776 -12.961 F2 3230513.000 3344957.667 272450.922 8.1 -13.303 F3 3148396.000 -11.187 F4 3655964.000 07 4.666 -20.527 G2 1415793.000 1405708.333 115406.937 8.2 -21.070 G3 1285590.000 -20.110 G4 1515742.000 08 2.799 -23.610 H2 676322.000 712664.667 108248.562 15.2 -23.814 H3 627264.000 -22.951 H4 834408.000 Group Summaries MinStd Smallest standard value: 712664.667 Min(MeanValue) MaxStd Largest standard value: 24278763.333 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A6 49940.000 R -26.222 -26.222 0.000 0.0 02 B6 71521.000 R -26.132 -26.132 0.000 0.0 03 C6 106301.000 R -25.987 -25.987 0.000 0.0 04 D6 200724.000 R -25.593 -25.593 0.000 0.0 05 E6 82577.000 R -26.085 -26.085 0.000 0.0 057 A1 35115.000 R -26.283 -26.283 0.000 0.0 058 B1 29150.000 R -26.308 -26.308 0.000 0.0 059 C1 25332.000 R -26.324 -26.324 0.000 0.0 06 F6 86623.000 R -26.069 -26.069 0.000 0.0 060 D1 40607.000 R -26.260 -26.260 0.000 0.0 061 E1 74821.000 R -26.118 -26.118 0.000 0.0 062 F1 28800.000 R -26.310 -26.310 0.000 0.0 063 G1 77213.000 R -26.108 -26.108 0.000 0.0 064 H1 65674.000 R -26.156 -26.156 0.000 0.0 065 A2 148158.000 R -25.812 -25.812 0.000 0.0 066 B2 105152.000 R -25.991 -25.991 0.000 0.0 067 C2 105903.000 R -25.988 -25.988 0.000 0.0 068 D2 117425.000 R -25.940 -25.940 0.000 0.0 069 E2 154497.000 R -25.786 -25.786 0.000 0.0 07 G6 92464.000 R -26.044 -26.044 0.000 0.0 070 F2 106028.000 R -25.988 -25.988 0.000 0.0 071 G2 175092.000 R -25.700 -25.700 0.000 0.0 072 H2 127627.000 R -25.898 -25.898 0.000 0.0 073 A3 100137.000 R -26.012 -26.012 0.000 0.0 074 B3 171601.000 R -25.714 -25.714 0.000 0.0 075 C3 108080.000 R -25.979 -25.979 0.000 0.0 076 D3 134217.000 R -25.870 -25.870 0.000 0.0 077 E3 126953.000 R -25.900 -25.900 0.000 0.0 078 F3 121310.000 R -25.924 -25.924 0.000 0.0 079 G3 9633.000 R -26.390 -26.390 0.000 0.0 08 H6 65462.000 R -26.157 -26.157 0.000 0.0 080 H3 7426.000 R -26.399 -26.399 0.000 0.0 081 A4 72884.000 R -26.126 -26.126 0.000 0.0 082 B4 51281.000 R -26.216 -26.216 0.000 0.0 083 C4 27330.000 R -26.316 -26.316 0.000 0.0 084 D4 38759.000 R -26.268 -26.268 0.000 0.0 085 E4 48302.000 R -26.228 -26.228 0.000 0.0 086 F4 37993.000 R -26.271 -26.271 0.000 0.0 087 G4 39287.000 R -26.266 -26.266 0.000 0.0 088 H4 45981.000 R -26.238 -26.238 0.000 0.0 089 A5 226668.000 R -25.485 -25.485 0.000 0.0 09 A7 27602.000 R -26.315 -26.315 0.000 0.0 090 B5 207420.000 R -25.565 -25.565 0.000 0.0 091 C5 208904.000 R -25.559 -25.559 0.000 0.0 092 D5 270142.000 R -25.303 -25.303 0.000 0.0 093 E5 271515.000 R -25.298 -25.298 0.000 0.0 094 F5 268164.000 R -25.312 -25.312 0.000 0.0 095 G5 248799.000 R -25.392 -25.392 0.000 0.0 096 H5 219669.000 R -25.514 -25.514 0.000 0.0 097 A6 124793.000 R -25.909 -25.909 0.000 0.0 098 B6 222859.000 R -25.501 -25.501 0.000 0.0 099 C6 106331.000 R -25.986 -25.986 0.000 0.0 10 B7 30476.000 R -26.303 -26.303 0.000 0.0 100 D6 180182.000 R -25.679 -25.679 0.000 0.0 101 E6 211539.000 R -25.548 -25.548 0.000 0.0 102 F6 159399.000 R -25.765 -25.765 0.000 0.0 103 G6 118416.000 R -25.936 -25.936 0.000 0.0 104 H6 86040.000 R -26.071 -26.071 0.000 0.0 105 A7 239123.000 R -25.433 -25.433 0.000 0.0 106 B7 15010.000 R -26.367 -26.367 0.000 0.0 107 C7 13445.000 R -26.374 -26.374 0.000 0.0 108 D7 182479.000 R -25.669 -25.669 0.000 0.0 109 E7 157336.000 R -25.774 -25.774 0.000 0.0 11 C7 35488.000 R -26.282 -26.282 0.000 0.0 110 F7 128257.000 R -25.895 -25.895 0.000 0.0 111 G7 15269.000 R -26.366 -26.366 0.000 0.0 112 H7 16263.000 R -26.362 -26.362 0.000 0.0 113 A8 192024.000 R -25.629 -25.629 0.000 0.0 114 B8 155730.000 R -25.780 -25.780 0.000 0.0 115 C8 198889.000 R -25.601 -25.601 0.000 0.0 116 D8 168811.000 R -25.726 -25.726 0.000 0.0 117 E8 163305.000 R -25.749 -25.749 0.000 0.0 118 F8 135714.000 R -25.864 -25.864 0.000 0.0 119 G8 156495.000 R -25.777 -25.777 0.000 0.0 12 D7 33521.000 R -26.290 -26.290 0.000 0.0 120 H8 157409.000 R -25.773 -25.773 0.000 0.0 121 A9 124743.000 R -25.910 -25.910 0.000 0.0 122 B9 103515.000 R -25.998 -25.998 0.000 0.0 123 C9 97582.000 R -26.023 -26.023 0.000 0.0 124 D9 160252.000 R -25.762 -25.762 0.000 0.0 125 E9 205605.000 R -25.573 -25.573 0.000 0.0 126 F9 240909.000 R -25.425 -25.425 0.000 0.0 127 G9 233480.000 R -25.456 -25.456 0.000 0.0 128 H9 220821.000 R -25.509 -25.509 0.000 0.0 129 A10 114306.000 R -25.953 -25.953 0.000 0.0 13 E7 32354.000 R -26.295 -26.295 0.000 0.0 130 B10 127240.000 R -25.899 -25.899 0.000 0.0 131 C10 58788.000 R -26.185 -26.185 0.000 0.0 132 D10 126725.000 R -25.901 -25.901 0.000 0.0 133 E10 159498.000 R -25.765 -25.765 0.000 0.0 134 F10 136951.000 R -25.859 -25.859 0.000 0.0 135 G10 233555.000 R -25.456 -25.456 0.000 0.0 136 H10 127079.000 R -25.900 -25.900 0.000 0.0 137 A11 177539.000 R -25.690 -25.690 0.000 0.0 138 B11 186209.000 R -25.653 -25.653 0.000 0.0 139 C11 223309.000 R -25.499 -25.499 0.000 0.0 14 F7 28980.000 R -26.309 -26.309 0.000 0.0 140 D11 140914.000 R -25.842 -25.842 0.000 0.0 141 E11 174670.000 R -25.701 -25.701 0.000 0.0 142 F11 156658.000 R -25.777 -25.777 0.000 0.0 143 G11 227490.000 R -25.481 -25.481 0.000 0.0 144 H11 180410.000 R -25.678 -25.678 0.000 0.0 145 A12 79816.000 R -26.097 -26.097 0.000 0.0 146 B12 9682.000 R -26.389 -26.389 0.000 0.0 147 C12 21971.000 R -26.338 -26.338 0.000 0.0 148 D12 26356.000 R -26.320 -26.320 0.000 0.0 149 E12 88281.000 R -26.062 -26.062 0.000 0.0 15 G7 45206.000 R -26.241 -26.241 0.000 0.0 150 F12 150002.000 R -25.804 -25.804 0.000 0.0 151 G12 168213.000 R -25.728 -25.728 0.000 0.0 152 H12 10727.000 R -26.385 -26.385 0.000 0.0 153 A5 10932.000 R -26.384 -26.386 0.001 0.0 B5 10488.000 R -26.386 C5 10659.000 R -26.385 D5 10140.000 R -26.387 E5 10381.000 R -26.386 F5 10699.000 R -26.385 G5 10673.000 R -26.385 H5 10487.000 R -26.386 16 H7 75799.000 R -26.114 -26.114 0.000 0.0 17 A8 54158.000 R -26.204 -26.204 0.000 0.0 18 B8 29640.000 R -26.306 -26.306 0.000 0.0 19 C8 22418.000 R -26.336 -26.336 0.000 0.0 20 D8 26135.000 R -26.321 -26.321 0.000 0.0 21 E8 25336.000 R -26.324 -26.324 0.000 0.0 22 F8 22848.000 R -26.334 -26.334 0.000 0.0 23 G8 28620.000 R -26.310 -26.310 0.000 0.0 24 H8 33353.000 R -26.291 -26.291 0.000 0.0 25 A9 39733.000 R -26.264 -26.264 0.000 0.0 26 B9 45334.000 R -26.241 -26.241 0.000 0.0 27 C9 57562.000 R -26.190 -26.190 0.000 0.0 28 D9 33710.000 R -26.289 -26.289 0.000 0.0 29 E9 51021.000 R -26.217 -26.217 0.000 0.0 30 F9 25266.000 R -26.324 -26.324 0.000 0.0 31 G9 31741.000 R -26.297 -26.297 0.000 0.0 32 H9 41273.000 R -26.258 -26.258 0.000 0.0 33 A10 21552.000 R -26.340 -26.340 0.000 0.0 34 B10 62917.000 R -26.167 -26.167 0.000 0.0 35 C10 56205.000 R -26.195 -26.195 0.000 0.0 36 D10 37935.000 R -26.272 -26.272 0.000 0.0 37 E10 19811.000 R -26.347 -26.347 0.000 0.0 38 F10 46991.000 R -26.234 -26.234 0.000 0.0 39 G10 35481.000 R -26.282 -26.282 0.000 0.0 40 H10 43083.000 R -26.250 -26.250 0.000 0.0 41 A11 98638.000 R -26.018 -26.018 0.000 0.0 42 B11 100175.000 R -26.012 -26.012 0.000 0.0 43 C11 101168.000 R -26.008 -26.008 0.000 0.0 44 D11 96188.000 R -26.029 -26.029 0.000 0.0 45 E11 13114.000 R -26.375 -26.375 0.000 0.0 46 F11 73336.000 R -26.124 -26.124 0.000 0.0 47 G11 102429.000 R -26.003 -26.003 0.000 0.0 48 H11 95125.000 R -26.033 -26.033 0.000 0.0 49 A12 87945.000 R -26.063 -26.063 0.000 0.0 50 B12 55231.000 R -26.199 -26.199 0.000 0.0 51 C12 65901.000 R -26.155 -26.155 0.000 0.0 52 D12 53447.000 R -26.207 -26.207 0.000 0.0 53 E12 56143.000 R -26.196 -26.196 0.000 0.0 54 F12 71956.000 R -26.130 -26.130 0.000 0.0 55 G12 38745.000 R -26.268 -26.268 0.000 0.0 56 H12 56999.000 R -26.192 -26.192 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: BothPlatesNoBlank_12-17-15; Date Last Saved: 12/17/2015 12:58:25 PM