##BLOCKS= 5 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 20726.5 128677.5 85139.5 69040.5 224225.5 144753.5 254788.5 201012.5 118432.5 141494.5 175921.5 65413.5 19701.5 94635.5 217263.5 35580.5 175657.5 222377.5 3597.5 124057.5 90189.5 64880.5 196183.5 -421.5 14756.5 99489.5 97228.5 13650.5 193550.5 123353.5 2729.5 188052.5 90882.5 51150.5 200832.5 13142.5 29809.5 132277.5 132012.5 25285.5 263430.5 171314.5 150369.5 132608.5 151218.5 110171.5 109788.5 19011.5 66007.5 191337.5 117970.5 36918.5 233172.5 214327.5 154725.5 173255.5 211586.5 175424.5 201076.5 81291.5 18488.5 103187.5 109332.5 25094.5 260976.5 157440.5 123550.5 131109.5 220082.5 143745.5 182140.5 137905.5 70108.5 189752.5 -713.5 28409.5 285279.5 139578.5 5253.5 154701.5 230817.5 246577.5 262404.5 167659.5 58430.5 130993.5 -3126.5 35462.5 214061.5 82543.5 6360.5 156711.5 227741.5 115836.5 194539.5 -422.5 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 1171 18502630 18331430 22039570 -38.5 40996.5 18294.5 46305.5 31295.5 12640.5 102217.5 119462.5 1021 27738478 27596058 31726292 109.5 67429.5 21411.5 23311.5 37508.5 40019.5 100216.5 46720.5 1218 25344500 23072144 33023282 -344.5 104808.5 27245.5 12287.5 49981.5 56969.5 93745.5 50443.5 1130 28737572 22814284 25144496 41.5 203120.5 24872.5 16623.5 27825.5 29631.5 90049.5 45254.5 1051 9399864 12322404 24891856 257.5 79512.5 23111.5 16683.5 43946.5 10874.5 3222.5 49452.5 1081 2681993 4030976 7968642 283.5 82013.5 19662.5 12676.5 12341.5 39104.5 64061.5 68413.5 1143 1043495 1977147 1836489 -63.5 88380.5 37530.5 20065.5 22389.5 27197.5 101380.5 30968.5 1115 784405 620641 679257 -245.5 59065.5 70579.5 24216.5 32875.5 39078.5 89588.5 53660.5 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 100.000 45.178 A2 18502630.000 19624543.333 2093225.432 10.7 44.351 A3 18331430.000 62.276 A4 22039570.000 02 60.000 89.826 B2 27738478.000 29020276.000 2344560.259 8.1 89.137 B3 27596058.000 109.104 B4 31726292.000 03 36.000 78.253 C2 25344500.000 27146642.000 5214601.971 19.2 67.268 C3 23072144.000 115.374 C4 33023282.000 04 21.600 94.656 D2 28737572.000 25565450.667 2983996.868 11.7 66.022 D3 22814284.000 77.286 D4 25144496.000 05 12.960 1.174 E2 9399864.000 15538041.333 8231384.855 53.0 15.302 E3 12322404.000 76.065 E4 24891856.000 06 7.776 -31.301 F2 2681993.000 4893870.333 2746926.352 56.1 -24.780 F3 4030976.000 -5.745 F4 7968642.000 07 4.666 -39.222 G2 1043495.000 1619043.667 503376.965 31.1 -34.709 G3 1977147.000 -35.389 G4 1836489.000 08 2.799 -40.475 H2 784405.000 694767.667 82976.488 11.9 -41.266 H3 620641.000 -40.983 H4 679257.000 Group Summaries MinStd Smallest standard value: 694767.667 Min(MeanValue) MaxStd Largest standard value: 29020276.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A6 40996.500 R -44.068 -44.068 0.000 0.0 02 B6 67429.500 R -43.941 -43.941 0.000 0.0 03 C6 104808.500 R -43.760 -43.760 0.000 0.0 04 D6 203120.500 R -43.285 -43.285 0.000 0.0 05 E6 79512.500 R -43.882 -43.882 0.000 0.0 057 A1 20726.500 R -44.166 -44.166 0.000 0.0 058 B1 19701.500 R -44.171 -44.171 0.000 0.0 059 C1 14756.500 R -44.195 -44.195 0.000 0.0 06 F6 82013.500 R -43.870 -43.870 0.000 0.0 060 D1 29809.500 R -44.122 -44.122 0.000 0.0 061 E1 66007.500 R -43.947 -43.947 0.000 0.0 062 F1 18488.500 R -44.177 -44.177 0.000 0.0 063 G1 70108.500 R -43.928 -43.928 0.000 0.0 064 H1 58430.500 R -43.984 -43.984 0.000 0.0 065 A2 128677.500 R -43.644 -43.644 0.000 0.0 066 B2 94635.500 R -43.809 -43.809 0.000 0.0 067 C2 99489.500 R -43.786 -43.786 0.000 0.0 068 D2 132277.500 R -43.627 -43.627 0.000 0.0 069 E2 191337.500 R -43.342 -43.342 0.000 0.0 07 G6 88380.500 R -43.839 -43.839 0.000 0.0 070 F2 103187.500 R -43.768 -43.768 0.000 0.0 071 G2 189752.500 R -43.349 -43.349 0.000 0.0 072 H2 130993.500 R -43.633 -43.633 0.000 0.0 073 A3 85139.500 R -43.855 -43.855 0.000 0.0 074 B3 217263.500 R -43.216 -43.216 0.000 0.0 075 C3 97228.500 R -43.796 -43.796 0.000 0.0 076 D3 132012.500 R -43.628 -43.628 0.000 0.0 077 E3 117970.500 R -43.696 -43.696 0.000 0.0 078 F3 109332.500 R -43.738 -43.738 0.000 0.0 079 G3 -713.500 R -44.270 -44.270 0.000 0.0 08 H6 59065.500 R -43.981 -43.981 0.000 0.0 080 H3 -3126.500 R -44.282 -44.282 0.000 0.0 081 A4 69040.500 R -43.933 -43.933 0.000 0.0 082 B4 35580.500 R -44.094 -44.094 0.000 0.0 083 C4 13650.500 R -44.200 -44.200 0.000 0.0 084 D4 25285.500 R -44.144 -44.144 0.000 0.0 085 E4 36918.500 R -44.088 -44.088 0.000 0.0 086 F4 25094.500 R -44.145 -44.145 0.000 0.0 087 G4 28409.500 R -44.129 -44.129 0.000 0.0 088 H4 35462.500 R -44.095 -44.095 0.000 0.0 089 A5 224225.500 R -43.183 -43.183 0.000 0.0 09 A7 18294.500 R -44.178 -44.178 0.000 0.0 090 B5 175657.500 R -43.417 -43.417 0.000 0.0 091 C5 193550.500 R -43.331 -43.331 0.000 0.0 092 D5 263430.500 R -42.993 -42.993 0.000 0.0 093 E5 233172.500 R -43.139 -43.139 0.000 0.0 094 F5 260976.500 R -43.005 -43.005 0.000 0.0 095 G5 285279.500 R -42.887 -42.887 0.000 0.0 096 H5 214061.500 R -43.232 -43.232 0.000 0.0 097 A6 144753.500 R -43.567 -43.567 0.000 0.0 098 B6 222377.500 R -43.191 -43.191 0.000 0.0 099 C6 123353.500 R -43.670 -43.670 0.000 0.0 10 B7 21411.500 R -44.163 -44.163 0.000 0.0 100 D6 171314.500 R -43.438 -43.438 0.000 0.0 101 E6 214327.500 R -43.230 -43.230 0.000 0.0 102 F6 157440.500 R -43.505 -43.505 0.000 0.0 103 G6 139578.500 R -43.592 -43.592 0.000 0.0 104 H6 82543.500 R -43.867 -43.867 0.000 0.0 105 A7 254788.500 R -43.035 -43.035 0.000 0.0 106 B7 3597.500 R -44.249 -44.249 0.000 0.0 107 C7 2729.500 R -44.253 -44.253 0.000 0.0 108 D7 150369.500 R -43.540 -43.540 0.000 0.0 109 E7 154725.500 R -43.518 -43.518 0.000 0.0 11 C7 27245.500 R -44.135 -44.135 0.000 0.0 110 F7 123550.500 R -43.669 -43.669 0.000 0.0 111 G7 5253.500 R -44.241 -44.241 0.000 0.0 112 H7 6360.500 R -44.236 -44.236 0.000 0.0 113 A8 201012.500 R -43.295 -43.295 0.000 0.0 114 B8 124057.500 R -43.667 -43.667 0.000 0.0 115 C8 188052.500 R -43.357 -43.357 0.000 0.0 116 D8 132608.500 R -43.625 -43.625 0.000 0.0 117 E8 173255.500 R -43.429 -43.429 0.000 0.0 118 F8 131109.500 R -43.633 -43.633 0.000 0.0 119 G8 154701.500 R -43.519 -43.519 0.000 0.0 12 D7 24872.500 R -44.146 -44.146 0.000 0.0 120 H8 156711.500 R -43.509 -43.509 0.000 0.0 121 A9 118432.500 R -43.694 -43.694 0.000 0.0 122 B9 90189.500 R -43.830 -43.830 0.000 0.0 123 C9 90882.500 R -43.827 -43.827 0.000 0.0 124 D9 151218.500 R -43.535 -43.535 0.000 0.0 125 E9 211586.500 R -43.244 -43.244 0.000 0.0 126 F9 220082.500 R -43.203 -43.203 0.000 0.0 127 G9 230817.500 R -43.151 -43.151 0.000 0.0 128 H9 227741.500 R -43.166 -43.166 0.000 0.0 129 A10 141494.500 R -43.582 -43.582 0.000 0.0 13 E7 23111.500 R -44.155 -44.155 0.000 0.0 130 B10 64880.500 R -43.953 -43.953 0.000 0.0 131 C10 51150.500 R -44.019 -44.019 0.000 0.0 132 D10 110171.500 R -43.734 -43.734 0.000 0.0 133 E10 175424.500 R -43.418 -43.418 0.000 0.0 134 F10 143745.500 R -43.572 -43.572 0.000 0.0 135 G10 246577.500 R -43.074 -43.074 0.000 0.0 136 H10 115836.500 R -43.706 -43.706 0.000 0.0 137 A11 175921.500 R -43.416 -43.416 0.000 0.0 138 B11 196183.500 R -43.318 -43.318 0.000 0.0 139 C11 200832.500 R -43.296 -43.296 0.000 0.0 14 F7 19662.500 R -44.171 -44.171 0.000 0.0 140 D11 109788.500 R -43.736 -43.736 0.000 0.0 141 E11 201076.500 R -43.294 -43.294 0.000 0.0 142 F11 182140.500 R -43.386 -43.386 0.000 0.0 143 G11 262404.500 R -42.998 -42.998 0.000 0.0 144 H11 194539.500 R -43.326 -43.326 0.000 0.0 145 A12 65413.500 R -43.950 -43.950 0.000 0.0 146 B12 -421.500 R -44.269 -44.269 0.000 0.0 147 C12 13142.500 R -44.203 -44.203 0.000 0.0 148 D12 19011.500 R -44.175 -44.175 0.000 0.0 149 E12 81291.500 R -43.873 -43.873 0.000 0.0 15 G7 37530.500 R -44.085 -44.085 0.000 0.0 150 F12 137905.500 R -43.600 -43.600 0.000 0.0 151 G12 167659.500 R -43.456 -43.456 0.000 0.0 152 H12 -422.500 R -44.269 -44.269 0.000 0.0 16 H7 70579.500 R -43.925 -43.925 0.000 0.0 17 A8 46305.500 R -44.043 -44.043 0.000 0.0 18 B8 23311.500 R -44.154 -44.154 0.000 0.0 19 C8 12287.500 R -44.207 -44.207 0.000 0.0 20 D8 16623.500 R -44.186 -44.186 0.000 0.0 21 E8 16683.500 R -44.186 -44.186 0.000 0.0 22 F8 12676.500 R -44.205 -44.205 0.000 0.0 23 G8 20065.500 R -44.169 -44.169 0.000 0.0 24 H8 24216.500 R -44.149 -44.149 0.000 0.0 25 A9 31295.500 R -44.115 -44.115 0.000 0.0 26 B9 37508.500 R -44.085 -44.085 0.000 0.0 27 C9 49981.500 R -44.025 -44.025 0.000 0.0 28 D9 27825.500 R -44.132 -44.132 0.000 0.0 29 E9 43946.500 R -44.054 -44.054 0.000 0.0 30 F9 12341.500 R -44.207 -44.207 0.000 0.0 31 G9 22389.500 R -44.158 -44.158 0.000 0.0 32 H9 32875.500 R -44.108 -44.108 0.000 0.0 33 A10 12640.500 R -44.205 -44.205 0.000 0.0 34 B10 40019.500 R -44.073 -44.073 0.000 0.0 35 C10 56969.500 R -43.991 -43.991 0.000 0.0 36 D10 29631.500 R -44.123 -44.123 0.000 0.0 37 E10 10874.500 R -44.214 -44.214 0.000 0.0 38 F10 39104.500 R -44.077 -44.077 0.000 0.0 39 G10 27197.500 R -44.135 -44.135 0.000 0.0 40 H10 39078.500 R -44.078 -44.078 0.000 0.0 41 A11 102217.500 R -43.772 -43.772 0.000 0.0 42 B11 100216.500 R -43.782 -43.782 0.000 0.0 43 C11 93745.500 R -43.813 -43.813 0.000 0.0 44 D11 90049.500 R -43.831 -43.831 0.000 0.0 45 E11 3222.500 R -44.251 -44.251 0.000 0.0 46 F11 64061.500 R -43.957 -43.957 0.000 0.0 47 G11 101380.500 R -43.776 -43.776 0.000 0.0 48 H11 89588.500 R -43.833 -43.833 0.000 0.0 49 A12 119462.500 R -43.689 -43.689 0.000 0.0 50 B12 46720.500 R -44.041 -44.041 0.000 0.0 51 C12 50443.500 R -44.023 -44.023 0.000 0.0 52 D12 45254.500 R -44.048 -44.048 0.000 0.0 53 E12 49452.500 R -44.027 -44.027 0.000 0.0 54 F12 68413.500 R -43.936 -43.936 0.000 0.0 55 G12 30968.500 R -44.117 -44.117 0.000 0.0 56 H12 53660.500 R -44.007 -44.007 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: BothPlates_12-17-15; Date Last Saved: 12/17/2015 12:52:40 PM