##BLOCKS= 5 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 31594 139545 96007 79908 235093 155621 265656 211880 129300 152362 186789 76281 30569 105503 228131 46448 186525 233245 14465 134925 101057 75748 207051 10446 25624 110357 108096 24518 204418 134221 13597 198920 101750 62018 211700 24010 40677 143145 142880 36153 274298 182182 161237 143476 162086 121039 120656 29879 76875 202205 128838 47786 244040 225195 165593 184123 222454 186292 211944 92159 29356 114055 120200 35962 271844 168308 134418 141977 230950 154613 193008 148773 80976 200620 10154 39277 296147 150446 16121 165569 241685 257445 273272 178527 69298 141861 7741 46330 224929 93411 17228 167579 238609 126704 205407 10445 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 1114 22647778 24512086 25676426 10932 49940 27602 54158 39733 21552 98638 87945 1150 20568052 24266164 23984500 10488 71521 30476 29640 45334 62917 100175 55231 1209 21992480 24841326 25580228 10659 106301 35488 22418 57562 56205 101168 65901 1188 21749368 15721731 21981776 10140 200724 33521 26135 33710 37935 96188 53447 1095 10416031 9878424 18796266 10381 82577 32354 25336 51021 19811 13114 56143 1120 3230513 3148396 3655964 10699 86623 28980 22848 25266 46991 73336 71956 1096 1415793 1285590 1515742 10673 92464 45206 28620 31741 35481 102429 38745 1176 676322 627264 834408 10487 65462 75799 33353 41273 43083 95125 56999 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 100.000 67.996 A2 22647778.000 24278763.333 1527745.652 6.3 75.769 A3 24512086.000 80.623 A4 25676426.000 02 60.000 59.325 B2 20568052.000 22939572.000 2058619.437 9.0 74.744 B3 24266164.000 73.569 B4 23984500.000 03 36.000 65.264 C2 21992480.000 24138011.333 1894458.384 7.8 77.142 C3 24841326.000 80.222 C4 25580228.000 04 21.600 64.250 D2 21749368.000 19817625.000 3549051.157 17.9 39.119 D3 15721731.000 65.219 D4 21981776.000 05 12.960 16.998 E2 10416031.000 13030240.333 5000754.374 38.4 14.757 E3 9878424.000 51.938 E4 18796266.000 06 7.776 -12.961 F2 3230513.000 3344957.667 272450.922 8.1 -13.303 F3 3148396.000 -11.187 F4 3655964.000 07 4.666 -20.527 G2 1415793.000 1405708.333 115406.937 8.2 -21.070 G3 1285590.000 -20.110 G4 1515742.000 08 2.799 -23.610 H2 676322.000 712664.667 108248.562 15.2 -23.814 H3 627264.000 -22.951 H4 834408.000 Group Summaries MinStd Smallest standard value: 712664.667 Min(MeanValue) MaxStd Largest standard value: 24278763.333 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A6 49940.000 R -26.222 -26.222 0.000 0.0 02 B6 71521.000 R -26.132 -26.132 0.000 0.0 03 C6 106301.000 R -25.987 -25.987 0.000 0.0 04 D6 200724.000 R -25.593 -25.593 0.000 0.0 05 E6 82577.000 R -26.085 -26.085 0.000 0.0 057 A1 31594.000 R -26.298 -26.298 0.000 0.0 058 B1 30569.000 R -26.302 -26.302 0.000 0.0 059 C1 25624.000 R -26.323 -26.323 0.000 0.0 06 F6 86623.000 R -26.069 -26.069 0.000 0.0 060 D1 40677.000 R -26.260 -26.260 0.000 0.0 061 E1 76875.000 R -26.109 -26.109 0.000 0.0 062 F1 29356.000 R -26.307 -26.307 0.000 0.0 063 G1 80976.000 R -26.092 -26.092 0.000 0.0 064 H1 69298.000 R -26.141 -26.141 0.000 0.0 065 A2 139545.000 R -25.848 -25.848 0.000 0.0 066 B2 105503.000 R -25.990 -25.990 0.000 0.0 067 C2 110357.000 R -25.970 -25.970 0.000 0.0 068 D2 143145.000 R -25.833 -25.833 0.000 0.0 069 E2 202205.000 R -25.587 -25.587 0.000 0.0 07 G6 92464.000 R -26.044 -26.044 0.000 0.0 070 F2 114055.000 R -25.954 -25.954 0.000 0.0 071 G2 200620.000 R -25.593 -25.593 0.000 0.0 072 H2 141861.000 R -25.838 -25.838 0.000 0.0 073 A3 96007.000 R -26.029 -26.029 0.000 0.0 074 B3 228131.000 R -25.479 -25.479 0.000 0.0 075 C3 108096.000 R -25.979 -25.979 0.000 0.0 076 D3 142880.000 R -25.834 -25.834 0.000 0.0 077 E3 128838.000 R -25.893 -25.893 0.000 0.0 078 F3 120200.000 R -25.929 -25.929 0.000 0.0 079 G3 10154.000 R -26.387 -26.387 0.000 0.0 08 H6 65462.000 R -26.157 -26.157 0.000 0.0 080 H3 7741.000 R -26.397 -26.397 0.000 0.0 081 A4 79908.000 R -26.097 -26.097 0.000 0.0 082 B4 46448.000 R -26.236 -26.236 0.000 0.0 083 C4 24518.000 R -26.328 -26.328 0.000 0.0 084 D4 36153.000 R -26.279 -26.279 0.000 0.0 085 E4 47786.000 R -26.231 -26.231 0.000 0.0 086 F4 35962.000 R -26.280 -26.280 0.000 0.0 087 G4 39277.000 R -26.266 -26.266 0.000 0.0 088 H4 46330.000 R -26.237 -26.237 0.000 0.0 089 A5 235093.000 R -25.450 -25.450 0.000 0.0 09 A7 27602.000 R -26.315 -26.315 0.000 0.0 090 B5 186525.000 R -25.652 -25.652 0.000 0.0 091 C5 204418.000 R -25.577 -25.577 0.000 0.0 092 D5 274298.000 R -25.286 -25.286 0.000 0.0 093 E5 244040.000 R -25.412 -25.412 0.000 0.0 094 F5 271844.000 R -25.296 -25.296 0.000 0.0 095 G5 296147.000 R -25.195 -25.195 0.000 0.0 096 H5 224929.000 R -25.492 -25.492 0.000 0.0 097 A6 155621.000 R -25.781 -25.781 0.000 0.0 098 B6 233245.000 R -25.457 -25.457 0.000 0.0 099 C6 134221.000 R -25.870 -25.870 0.000 0.0 10 B7 30476.000 R -26.303 -26.303 0.000 0.0 100 D6 182182.000 R -25.670 -25.670 0.000 0.0 101 E6 225195.000 R -25.491 -25.491 0.000 0.0 102 F6 168308.000 R -25.728 -25.728 0.000 0.0 103 G6 150446.000 R -25.802 -25.802 0.000 0.0 104 H6 93411.000 R -26.040 -26.040 0.000 0.0 105 A7 265656.000 R -25.322 -25.322 0.000 0.0 106 B7 14465.000 R -26.369 -26.369 0.000 0.0 107 C7 13597.000 R -26.373 -26.373 0.000 0.0 108 D7 161237.000 R -25.757 -25.757 0.000 0.0 109 E7 165593.000 R -25.739 -25.739 0.000 0.0 11 C7 35488.000 R -26.282 -26.282 0.000 0.0 110 F7 134418.000 R -25.869 -25.869 0.000 0.0 111 G7 16121.000 R -26.363 -26.363 0.000 0.0 112 H7 17228.000 R -26.358 -26.358 0.000 0.0 113 A8 211880.000 R -25.546 -25.546 0.000 0.0 114 B8 134925.000 R -25.867 -25.867 0.000 0.0 115 C8 198920.000 R -25.600 -25.600 0.000 0.0 116 D8 143476.000 R -25.832 -25.832 0.000 0.0 117 E8 184123.000 R -25.662 -25.662 0.000 0.0 118 F8 141977.000 R -25.838 -25.838 0.000 0.0 119 G8 165569.000 R -25.739 -25.739 0.000 0.0 12 D7 33521.000 R -26.290 -26.290 0.000 0.0 120 H8 167579.000 R -25.731 -25.731 0.000 0.0 121 A9 129300.000 R -25.891 -25.891 0.000 0.0 122 B9 101057.000 R -26.008 -26.008 0.000 0.0 123 C9 101750.000 R -26.006 -26.006 0.000 0.0 124 D9 162086.000 R -25.754 -25.754 0.000 0.0 125 E9 222454.000 R -25.502 -25.502 0.000 0.0 126 F9 230950.000 R -25.467 -25.467 0.000 0.0 127 G9 241685.000 R -25.422 -25.422 0.000 0.0 128 H9 238609.000 R -25.435 -25.435 0.000 0.0 129 A10 152362.000 R -25.794 -25.794 0.000 0.0 13 E7 32354.000 R -26.295 -26.295 0.000 0.0 130 B10 75748.000 R -26.114 -26.114 0.000 0.0 131 C10 62018.000 R -26.171 -26.171 0.000 0.0 132 D10 121039.000 R -25.925 -25.925 0.000 0.0 133 E10 186292.000 R -25.653 -25.653 0.000 0.0 134 F10 154613.000 R -25.785 -25.785 0.000 0.0 135 G10 257445.000 R -25.356 -25.356 0.000 0.0 136 H10 126704.000 R -25.901 -25.901 0.000 0.0 137 A11 186789.000 R -25.651 -25.651 0.000 0.0 138 B11 207051.000 R -25.566 -25.566 0.000 0.0 139 C11 211700.000 R -25.547 -25.547 0.000 0.0 14 F7 28980.000 R -26.309 -26.309 0.000 0.0 140 D11 120656.000 R -25.927 -25.927 0.000 0.0 141 E11 211944.000 R -25.546 -25.546 0.000 0.0 142 F11 193008.000 R -25.625 -25.625 0.000 0.0 143 G11 273272.000 R -25.290 -25.290 0.000 0.0 144 H11 205407.000 R -25.573 -25.573 0.000 0.0 145 A12 76281.000 R -26.112 -26.112 0.000 0.0 146 B12 10446.000 R -26.386 -26.386 0.000 0.0 147 C12 24010.000 R -26.330 -26.330 0.000 0.0 148 D12 29879.000 R -26.305 -26.305 0.000 0.0 149 E12 92159.000 R -26.046 -26.046 0.000 0.0 15 G7 45206.000 R -26.241 -26.241 0.000 0.0 150 F12 148773.000 R -25.809 -25.809 0.000 0.0 151 G12 178527.000 R -25.685 -25.685 0.000 0.0 152 H12 10445.000 R -26.386 -26.386 0.000 0.0 153 A5 10932.000 R -26.384 -26.386 0.001 0.0 B5 10488.000 R -26.386 C5 10659.000 R -26.385 D5 10140.000 R -26.387 E5 10381.000 R -26.386 F5 10699.000 R -26.385 G5 10673.000 R -26.385 H5 10487.000 R -26.386 16 H7 75799.000 R -26.114 -26.114 0.000 0.0 17 A8 54158.000 R -26.204 -26.204 0.000 0.0 18 B8 29640.000 R -26.306 -26.306 0.000 0.0 19 C8 22418.000 R -26.336 -26.336 0.000 0.0 20 D8 26135.000 R -26.321 -26.321 0.000 0.0 21 E8 25336.000 R -26.324 -26.324 0.000 0.0 22 F8 22848.000 R -26.334 -26.334 0.000 0.0 23 G8 28620.000 R -26.310 -26.310 0.000 0.0 24 H8 33353.000 R -26.291 -26.291 0.000 0.0 25 A9 39733.000 R -26.264 -26.264 0.000 0.0 26 B9 45334.000 R -26.241 -26.241 0.000 0.0 27 C9 57562.000 R -26.190 -26.190 0.000 0.0 28 D9 33710.000 R -26.289 -26.289 0.000 0.0 29 E9 51021.000 R -26.217 -26.217 0.000 0.0 30 F9 25266.000 R -26.324 -26.324 0.000 0.0 31 G9 31741.000 R -26.297 -26.297 0.000 0.0 32 H9 41273.000 R -26.258 -26.258 0.000 0.0 33 A10 21552.000 R -26.340 -26.340 0.000 0.0 34 B10 62917.000 R -26.167 -26.167 0.000 0.0 35 C10 56205.000 R -26.195 -26.195 0.000 0.0 36 D10 37935.000 R -26.272 -26.272 0.000 0.0 37 E10 19811.000 R -26.347 -26.347 0.000 0.0 38 F10 46991.000 R -26.234 -26.234 0.000 0.0 39 G10 35481.000 R -26.282 -26.282 0.000 0.0 40 H10 43083.000 R -26.250 -26.250 0.000 0.0 41 A11 98638.000 R -26.018 -26.018 0.000 0.0 42 B11 100175.000 R -26.012 -26.012 0.000 0.0 43 C11 101168.000 R -26.008 -26.008 0.000 0.0 44 D11 96188.000 R -26.029 -26.029 0.000 0.0 45 E11 13114.000 R -26.375 -26.375 0.000 0.0 46 F11 73336.000 R -26.124 -26.124 0.000 0.0 47 G11 102429.000 R -26.003 -26.003 0.000 0.0 48 H11 95125.000 R -26.033 -26.033 0.000 0.0 49 A12 87945.000 R -26.063 -26.063 0.000 0.0 50 B12 55231.000 R -26.199 -26.199 0.000 0.0 51 C12 65901.000 R -26.155 -26.155 0.000 0.0 52 D12 53447.000 R -26.207 -26.207 0.000 0.0 53 E12 56143.000 R -26.196 -26.196 0.000 0.0 54 F12 71956.000 R -26.130 -26.130 0.000 0.0 55 G12 38745.000 R -26.268 -26.268 0.000 0.0 56 H12 56999.000 R -26.192 -26.192 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: Plate1NoBlank_12-17-15; Date Last Saved: 12/17/2015 12:55:40 PM