##BLOCKS= 4 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 1171 18502630 18331430 22039570 -38.5 40996.5 18294.5 46305.5 31295.5 12640.5 102217.5 119462.5 1021 27738478 27596058 31726292 109.5 67429.5 21411.5 23311.5 37508.5 40019.5 100216.5 46720.5 1218 25344500 23072144 33023282 -344.5 104808.5 27245.5 12287.5 49981.5 56969.5 93745.5 50443.5 1130 28737572 22814284 25144496 41.5 203120.5 24872.5 16623.5 27825.5 29631.5 90049.5 45254.5 1051 9399864 12322404 24891856 257.5 79512.5 23111.5 16683.5 43946.5 10874.5 3222.5 49452.5 1081 2681993 4030976 7968642 283.5 82013.5 19662.5 12676.5 12341.5 39104.5 64061.5 68413.5 1143 1043495 1977147 1836489 -63.5 88380.5 37530.5 20065.5 22389.5 27197.5 101380.5 30968.5 1115 784405 620641 679257 -245.5 59065.5 70579.5 24216.5 32875.5 39078.5 89588.5 53660.5 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 100.000 45.178 A2 18502630.000 19624543.333 2093225.432 10.7 44.351 A3 18331430.000 62.276 A4 22039570.000 02 60.000 89.826 B2 27738478.000 29020276.000 2344560.259 8.1 89.137 B3 27596058.000 109.104 B4 31726292.000 03 36.000 78.253 C2 25344500.000 27146642.000 5214601.971 19.2 67.268 C3 23072144.000 115.374 C4 33023282.000 04 21.600 94.656 D2 28737572.000 25565450.667 2983996.868 11.7 66.022 D3 22814284.000 77.286 D4 25144496.000 05 12.960 1.174 E2 9399864.000 15538041.333 8231384.855 53.0 15.302 E3 12322404.000 76.065 E4 24891856.000 06 7.776 -31.301 F2 2681993.000 4893870.333 2746926.352 56.1 -24.780 F3 4030976.000 -5.745 F4 7968642.000 07 4.666 -39.222 G2 1043495.000 1619043.667 503376.965 31.1 -34.709 G3 1977147.000 -35.389 G4 1836489.000 08 2.799 -40.475 H2 784405.000 694767.667 82976.488 11.9 -41.266 H3 620641.000 -40.983 H4 679257.000 Group Summaries MinStd Smallest standard value: 694767.667 Min(MeanValue) MaxStd Largest standard value: 29020276.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A6 40996.500 R -44.068 -44.068 0.000 0.0 02 B6 67429.500 R -43.941 -43.941 0.000 0.0 03 C6 104808.500 R -43.760 -43.760 0.000 0.0 04 D6 203120.500 R -43.285 -43.285 0.000 0.0 05 E6 79512.500 R -43.882 -43.882 0.000 0.0 057 A1 R 058 B1 R 059 C1 R 06 F6 82013.500 R -43.870 -43.870 0.000 0.0 060 D1 R 061 E1 R 062 F1 R 063 G1 R 064 H1 R 065 A2 R 066 B2 R 067 C2 R 068 D2 R 069 E2 R 07 G6 88380.500 R -43.839 -43.839 0.000 0.0 070 F2 R 071 G2 R 072 H2 R 073 A3 R 074 B3 R 075 C3 R 076 D3 R 077 E3 R 078 F3 R 079 G3 R 08 H6 59065.500 R -43.981 -43.981 0.000 0.0 080 H3 R 081 A4 R 082 B4 R 083 C4 R 084 D4 R 085 E4 R 086 F4 R 087 G4 R 088 H4 R 089 A5 R 09 A7 18294.500 R -44.178 -44.178 0.000 0.0 090 B5 R 091 C5 R 092 D5 R 093 E5 R 094 F5 R 095 G5 R 096 H5 R 097 A6 R 098 B6 R 099 C6 R 10 B7 21411.500 R -44.163 -44.163 0.000 0.0 100 D6 R 101 E6 R 102 F6 R 103 G6 R 104 H6 R 105 A7 R 106 B7 R 107 C7 R 108 D7 R 109 E7 R 11 C7 27245.500 R -44.135 -44.135 0.000 0.0 110 F7 R 111 G7 R 112 H7 R 113 A8 R 114 B8 R 115 C8 R 116 D8 R 117 E8 R 118 F8 R 119 G8 R 12 D7 24872.500 R -44.146 -44.146 0.000 0.0 120 H8 R 121 A9 R 122 B9 R 123 C9 R 124 D9 R 125 E9 R 126 F9 R 127 G9 R 128 H9 R 129 A10 R 13 E7 23111.500 R -44.155 -44.155 0.000 0.0 130 B10 R 131 C10 R 132 D10 R 133 E10 R 134 F10 R 135 G10 R 136 H10 R 137 A11 R 138 B11 R 139 C11 R 14 F7 19662.500 R -44.171 -44.171 0.000 0.0 140 D11 R 141 E11 R 142 F11 R 143 G11 R 144 H11 R 145 A12 R 146 B12 R 147 C12 R 148 D12 R 149 E12 R 15 G7 37530.500 R -44.085 -44.085 0.000 0.0 150 F12 R 151 G12 R 152 H12 R 16 H7 70579.500 R -43.925 -43.925 0.000 0.0 17 A8 46305.500 R -44.043 -44.043 0.000 0.0 18 B8 23311.500 R -44.154 -44.154 0.000 0.0 19 C8 12287.500 R -44.207 -44.207 0.000 0.0 20 D8 16623.500 R -44.186 -44.186 0.000 0.0 21 E8 16683.500 R -44.186 -44.186 0.000 0.0 22 F8 12676.500 R -44.205 -44.205 0.000 0.0 23 G8 20065.500 R -44.169 -44.169 0.000 0.0 24 H8 24216.500 R -44.149 -44.149 0.000 0.0 25 A9 31295.500 R -44.115 -44.115 0.000 0.0 26 B9 37508.500 R -44.085 -44.085 0.000 0.0 27 C9 49981.500 R -44.025 -44.025 0.000 0.0 28 D9 27825.500 R -44.132 -44.132 0.000 0.0 29 E9 43946.500 R -44.054 -44.054 0.000 0.0 30 F9 12341.500 R -44.207 -44.207 0.000 0.0 31 G9 22389.500 R -44.158 -44.158 0.000 0.0 32 H9 32875.500 R -44.108 -44.108 0.000 0.0 33 A10 12640.500 R -44.205 -44.205 0.000 0.0 34 B10 40019.500 R -44.073 -44.073 0.000 0.0 35 C10 56969.500 R -43.991 -43.991 0.000 0.0 36 D10 29631.500 R -44.123 -44.123 0.000 0.0 37 E10 10874.500 R -44.214 -44.214 0.000 0.0 38 F10 39104.500 R -44.077 -44.077 0.000 0.0 39 G10 27197.500 R -44.135 -44.135 0.000 0.0 40 H10 39078.500 R -44.078 -44.078 0.000 0.0 41 A11 102217.500 R -43.772 -43.772 0.000 0.0 42 B11 100216.500 R -43.782 -43.782 0.000 0.0 43 C11 93745.500 R -43.813 -43.813 0.000 0.0 44 D11 90049.500 R -43.831 -43.831 0.000 0.0 45 E11 3222.500 R -44.251 -44.251 0.000 0.0 46 F11 64061.500 R -43.957 -43.957 0.000 0.0 47 G11 101380.500 R -43.776 -43.776 0.000 0.0 48 H11 89588.500 R -43.833 -43.833 0.000 0.0 49 A12 119462.500 R -43.689 -43.689 0.000 0.0 50 B12 46720.500 R -44.041 -44.041 0.000 0.0 51 C12 50443.500 R -44.023 -44.023 0.000 0.0 52 D12 45254.500 R -44.048 -44.048 0.000 0.0 53 E12 49452.500 R -44.027 -44.027 0.000 0.0 54 F12 68413.500 R -43.936 -43.936 0.000 0.0 55 G12 30968.500 R -44.117 -44.117 0.000 0.0 56 H12 53660.500 R -44.007 -44.007 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: Plate1_12-17-15; Date Last Saved: 12/17/2015 12:49:26 PM