Load time: 11/30/2007 11:17:25 AM Read time: 11/30/2007 11:17:31 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: BCA_Farnham_490 Plate ID: #1368 Comment: new comment Microplate format: corning_3596_96well Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: a3 Light sensor: PhotoDiode Excitation side: Top Emission side: Top Lamp: Continuous Flash lamp voltage: Readings per well: 3 Time between readings: 100 ms Integration time: 4156 us Delay after flash: Motion settling time: 0 ms Z Height: 5.335 mm Middle Excitation filter: 1 Fluorescein 485 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Photodiode capacitor: 1 Photodiode resistor: 5 Photodiode gain: x1 Photodiode remote gain: x100 Max cps: 15651 cps Min counts: 5032 counts Dark Count reading: 22 counts Well list: r0(b1:g3,a4:g6,a1:a3) = cps(a3(b1:g3,a4:g6,a1:a3)) Data: RAW DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 14205 15651 15647 7913 7065 7795 B 15268 14728 14908 8581 8446 8491 C 13894 14203 14533 7122 5032 5570 D 12331 12543 13018 7722 6952 7894 E 11355 11427 11500 12422 10480 7880 F 11600 11566 12140 9306 10005 10933 G 10361 10899 10891 11300 10841 11353 H Well list: r1(b1:g3,a4:g6) = Counts(a3(b1:g3,a4:g6)-22) Data: DARKCOUNT SUBTRACTED DATA Units: Counts Display format: %.0f 1 2 3 4 5 6 7 8 9 10 11 12 A 7891 7043 7773 B 15246 14706 14886 8559 8424 8469 C 13872 14181 14511 7100 5010 5548 D 12309 12521 12996 7700 6930 7872 E 11333 11405 11478 12400 10458 7858 F 11578 11544 12118 9284 9983 10911 G 10339 10877 10869 11278 10819 11331 H Well list: r2(b1:g3,a4:g6) = log10((avg(a3(a1:a3))-22)/(a3(b1:g3,a4:g6) - 22)),r2(a1:a3) = log10((avg(a3(a1:a3))-22)/(a3(a1:a3) - 22)) Data: Log10( (Buffer 1 - Dark counts) / (Sample 1 - Dark counts)) Units: O.D. Display format: %0.3f 1 2 3 4 5 6 7 8 9 10 11 12 A 0.029 -0.014 -0.014 0.283 0.333 0.290 B -0.003 0.013 0.008 0.248 0.255 0.252 C 0.038 0.029 0.019 0.329 0.480 0.436 D 0.090 0.083 0.066 0.294 0.340 0.284 E 0.126 0.123 0.120 0.087 0.161 0.285 F 0.117 0.118 0.097 0.213 0.181 0.142 G 0.166 0.144 0.144 0.128 0.146 0.126 H